Anti-CXCL1/GRO alpha antibody [EPR28745-123] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL1/GRO alpha antibody [EPR28745-123] - BSA and Azide free (AB324777)

This data was developed using ab322200, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a mouse CXCL1 expression vector containing a Myc-His tag. (B) HEK-293T transfected with a mouse CXCL2 expression vector containing a Myc-His tag. (C) HEK-293T transfected with empty vector containing a Myc-His tag. tissue labeling CXCL1/GRO alpha with ab322200 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on HEK-293T transfected with a Myc-His-tagged mouse CXCL1 construct (image A). No staining on HEK-293T transfected with a Myc-His-tagged mouse CXCL2 construct (image B). No staining on HEK-293T transfected with empty plasmid (image C). The section was incubated with ab322200 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL1/GRO alpha antibody [EPR28745-123] - BSA and Azide free (AB324777)

This data was developed using ab322200, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling CXCL1/GRO alpha with ab322200 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : no staining on mouse liver. The section was incubated with ab322200 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCL1/GRO alpha antibody [EPR28745-123] - BSA and Azide free (AB324777)

This data was developed using ab322200, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Mouse lung treated with lipopolysaccharide (LPS 1 ug/mL) and Brefeldin A (1 ug/mL) for 16 hours. (B) Untreated mouse lung. tissue labeling CXCL1/GRO alpha with ab322200 at 1/2000 (0.261 ug/ml) dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining in mouse lung treated lipopolysaccharide (LPS 1 ug/mL) and Brefeldin A (1 ug/mL) for 16 hours. No staining in (B) untreated mouse lung. The section was incubated with ab322200 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-CXCL1/GRO alpha antibody [EPR28745-123] - BSA and Azide free (AB324777)

This data was developed using ab322200, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-CXCL1/GRO alpha antibody [EPR28745-123] (<a href='/ko/products/primary-antibodies/cxcl1-gro-alpha-antibody-epr28745-123-ab322200'>ab322200</a>) at 1/1000 dilution

Lane 1:

Untreated mouse lung tissue lysate at 20 µg

Lane 2:

Mouse lung tissue treated with 1 ug/ml LPS(Lipopolysaccharide) and 1 ug/ml BFA(Brefeldin A) lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 10 kDa,36 kDa

false

Exposure time: 81s

• WB

Supplier Data

Western blot - Anti-CXCL1/GRO alpha antibody [EPR28745-123] - BSA and Azide free (AB324777)

This data was developed using ab322200, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed mouse CXCL2 or CXCL3 by western blot.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.

All lanes:

Western blot - Anti-CXCL1/GRO alpha antibody [EPR28745-123] (<a href='/ko/products/primary-antibodies/cxcl1-gro-alpha-antibody-epr28745-123-ab322200'>ab322200</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 2:

293T cells transfected with a mouse CXCL1 containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 3:

293T cells transfected with a mouse CXCL2 containing a myc-His-tag®, whole cell lysate at 10 µg

Lane 4:

293T cells transfected with a mouse CXCL3 containing a myc-His-tag®, whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 10 kDa,36 kDa

false

Exposure time: 81s