AB317157

Anti-CXCR7 antibody [HL2189]

Recombinant
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Rabbit Recombinant Monoclonal GPCR RDC1/CXCR-7 antibody. Suitable for ICC/IF, IHC-P, WB and reacts with Human, Mouse, Rat samples. Immunogen corresponding to Synthetic Peptide within Human ACKR3.

대체 명칭 보기

CMKOR1, CXCR7, GPR159, RDC1, ACKR3, Atypical chemokine receptor 3, C-X-C chemokine receptor type 7, Chemokine orphan receptor 1, G-protein coupled receptor 159, G-protein coupled receptor RDC1 homolog, CXC-R7, CXCR-7, RDC-1

9 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR7 antibody [HL2189] (AB317157)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR7 antibody [HL2189] (AB317157)

ab317157 detects CXCR7 protein at cell membrane by immunohistochemical analysis. Sample : Paraffin-embedded human colon cancer. CXCR7 stained by ab317157 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

Immunocytochemistry/ Immunofluorescence - Anti-CXCR7 antibody [HL2189] (AB317157)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CXCR7 antibody [HL2189] (AB317157)

Immunofluorescence analysis of THP-1 cells fixed in 4% paraformaldehyde at RT for 15 min labelling CXCR7 protein using ab317157 at a 1/100 dilution (Green).
DAPI was used for nuclear staining (Blue).

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR7 antibody [HL2189] (AB317157)

ab317157 detects CXCR7 protein at cell membrane by immunohistochemical analysis. Sample : Paraffin-embedded Raji xenograft. CXCR7 stained by ab317157 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CXCR7 antibody [HL2189] (AB317157)

Immunofluorescence analysis of Mock and transfected 293T cells fixed in 4% paraformaldehyde at RT for 15 min labelling CXCR7 protein using ab317157 at a 1/500 dilution (Green).
DAPI was used for nuclear staining (Blue). Scale bar= 10µm.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR7 antibody [HL2189] (AB317157)

ab317157 detects CXCR7 protein at cell membrane by immunohistochemical analysis. Sample : Paraffin-embedded rat colon. CXCR7 stained by ab317157 diluted at 1 : 100. Antigen Retrieval : Citrate buffer pH 6.0 15 min.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR7 antibody [HL2189] (AB317157)

Immunohistochemical analysis of paraffin-embedded mouse tissues labelling CXCR7 protein with ab317157 at a 1/100 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR7 antibody [HL2189] (AB317157)

Immunohistochemical analysis of paraffin-embedded rat tissues labelling CXCR7 protein with ab317157 at a 1/1000 dilution.
Antigen retrieval : Citrate buffer, pH 6.0, 15 min

Supplier Data

Western blot - Anti-CXCR7 antibody [HL2189] (AB317157)

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317157 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CXCR7 antibody [HL2189] (ab317157) at 1/1000 dilution

All lanes:

Rat colon tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

Supplier Data

Western blot - Anti-CXCR7 antibody [HL2189] (AB317157)

Mouse tissue extract (50 ug) was separated by 10% SDS-PAGE and the membrane was blotted with ab317157 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CXCR7 antibody [HL2189] (ab317157) at 1/1000 dilution

All lanes:

Mouse spleen tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2189

Isotype

IgG

Carrier free

Reacts with

Human, Mouse, Rat

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ACKR3.

P25106

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

보관 버퍼

pH: 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GPCR RDC1 also known as CXCR-7 is a G protein-coupled receptor that binds to chemokines such as CXCL12 and CXCL11. The receptor has a predicted molecular mass of approximately 40 kDa. It expresses widely in various tissues with high levels found in the heart liver kidney and brain. This receptor plays a role in the trafficking and localization of different cells often working alongside or complementing other chemokine receptors.

Biological function summary

GPCR RDC1/CXCR-7 takes part in complex cell signaling related to cell growth survival and migration. It is not usually part of a classic GPCR signaling pathway which sets it apart from other receptors in its family. Instead it can modulate pathways by forming heterodimers with other receptors such as CXCR4 affecting both ligand availability and receptor sensitivity. CXCR-7 can also function as a decoy receptor binding ligands without initiating a typical signaling cascade.

Pathways

The participation of GPCR RDC1/CXCR-7 is noted in chemokine-mediated pathways. Of particular importance is the SDF-1/CXCL12 signaling pathway which it shares with CXCR4. This pathway plays an important role in the regulation of stem cell migration and homing. CXCR-7 modulates the bioavailability of CXCL12 and influences how CXCR4 triggers downstream effects affecting cellular response to the external environment.

GPCR RDC1/CXCR-7 holds significance in cancer and cardiovascular diseases. In cancer overexpression of CXCR-7 associates with tumor growth and metastasis often in conjunction with the action of CXCR4. In cardiovascular conditions altered expression and functional shifts in CXCR-7 can lead to abnormal vascularization contributing to disease progression. Its role in these diseases makes CXCR-7 a valuable target for therapeutic interventions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Acts as a receptor for chemokines CXCL11 and CXCL12/SDF1 (PubMed : 16107333, PubMed : 19255243, PubMed : 19380869, PubMed : 20161793, PubMed : 22300987). Chemokine binding does not activate G-protein-mediated signal transduction but instead induces beta-arrestin recruitment, leading to ligand internalization and activation of MAPK signaling pathway (PubMed : 16940167, PubMed : 18653785, PubMed : 20018651). Required for regulation of CXCR4 protein levels in migrating interneurons, thereby adapting their chemokine responsiveness (PubMed : 16940167, PubMed : 18653785). In glioma cells, transduces signals via MEK/ERK pathway, mediating resistance to apoptosis. Promotes cell growth and survival (PubMed : 16940167, PubMed : 20388803). Not involved in cell migration, adhesion or proliferation of normal hematopoietic progenitors but activated by CXCL11 in malignant hemapoietic cells, leading to phosphorylation of ERK1/2 (MAPK3/MAPK1) and enhanced cell adhesion and migration (PubMed : 17804806, PubMed : 18653785, PubMed : 19641136, PubMed : 20887389). Plays a regulatory role in CXCR4-mediated activation of cell surface integrins by CXCL12 (PubMed : 18653785). Required for heart valve development (PubMed : 17804806). Regulates axon guidance in the oculomotor system through the regulation of CXCL12 levels (PubMed : 31211835).. (Microbial infection) Acts as a coreceptor with CXCR4 for a restricted number of HIV isolates.

See full target information ACKR3

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CXCR7 antibody [HL2189]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR7 antibody [HL2189] (AB317157)

Immunocytochemistry/ Immunofluorescence - Anti-CXCR7 antibody [HL2189] (AB317157)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR7 antibody [HL2189] (AB317157)

Immunocytochemistry/ Immunofluorescence - Anti-CXCR7 antibody [HL2189] (AB317157)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR7 antibody [HL2189] (AB317157)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR7 antibody [HL2189] (AB317157)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CXCR7 antibody [HL2189] (AB317157)

Western blot - Anti-CXCR7 antibody [HL2189] (AB317157)

Western blot - Anti-CXCR7 antibody [HL2189] (AB317157)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise