AB270940

Anti-Cyclin A1 antibody [EPR24373-146]

20ul selling size
RabMAb
Recombinant
각 태그의 뜻

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(7 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Cyclin A1 antibody. Suitable for WB, IHC-P and reacts with Recombinant fragment - Human, Human samples. Cited in 7 publications.

대체 명칭 보기

Cyclin-A1, CCNA1

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 antibody [EPR24373-146] (AB270940)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 antibody [EPR24373-146] (AB270940)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labelling Cyclin A1 with ab270940 at 1/2000 (0.231 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control : no staining on human pancreas (PMID : 9041194). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 antibody [EPR24373-146] (AB270940)

Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling Cyclin A1 with ab270940 at 1/2000 (0.231 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Nuclear staining on the pachytene spermatocytes of human testis (PMID : 14696091). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 antibody [EPR24373-146] (AB270940)

Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labelling Cyclin A1 with ab270940 at 1/2000 (0.231 ug/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Negative control : no staining on human cardiac muscle (PMID : 9041194). Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0)

Lab

Western blot - Anti-Cyclin A1 antibody [EPR24373-146] (AB270940)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes

All lanes:

Western blot - Anti-Cyclin A1 antibody [EPR24373-146] (ab270940) at 1/1000 dilution

All lanes:

Human testis tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 52 kDa

false

Lab

Western blot - Anti-Cyclin A1 antibody [EPR24373-146] (AB270940)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 10 seconds

All lanes:

Western blot - Anti-Cyclin A1 antibody [EPR24373-146] (ab270940) at 1/5000 dilution

Lane 1:

His-tagged human cyclinA1 recombinant protein (aa51-223)

Lane 2:

His-tagged human cyclinA2 recombinant protein (aa1-431)

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 52 kDa

Observed band size: 28 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24373-146

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

Not suitable for testing cell lines in WB.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/2000", "IHCP-species-notes": "" }, "Recombinant fragment - Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

May be involved in the control of the cell cycle at the G1/S (start) and G2/M (mitosis) transitions. May primarily function in the control of the germline meiotic cell cycle and additionally in the control of mitotic cell cycle in some somatic cells.

See full target information CCNA1

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in pharmacology 16:1588722 PubMed40771929

2025

Dual PI3K/mTOR inhibitor NVP-BEZ235 induces cell cycle arrest via autophagy mediated protein degradation of RPL19 in nephroblastoma cell.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Gao,Xinran Xing,Ruizhi Cai,Dong Liu,Qili Feng,Jiaqi Luo,Yongzhao Zhu,Zeli Su

Cancer medicine 14:e70958 PubMed40567110

2025

STOX1 Isoform A Promotes Proliferation and Progression of Hepatocellular Carcinoma by Dual Mechanisms of Transcriptionally Upregulation of Cyclin B1 and Activation of ROS-Dependent PTEN/AKT1 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chunlin Jiang,Chong Wang,Jian Ao,Yangping Liu,Fengjie Sun,Wangpan Shi,Zeyi Guo,Yanping Wu,Luxiang Gan,Meimei Wu,Yaofeng Zhi,Zijie Meng,Wanting Wu,Juanhua Wu,Yong Ye,Xin Zhang,Dong Ren,Mingxin Pan

Scientific reports 14:21701 PubMed39289424

2024

Y-box binding protein 1/cyclin A1 axis specifically promotes cell cycle progression at G/M phase in ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Yuichi Murakami,Daisuke Katsuchi,Taichi Matsumoto,Kuon Kanazawa,Tomohiro Shibata,Akihiko Kawahara,Jun Akiba,Nozomu Yanaihara,Aikou Okamoto,Hiroaki Itamochi,Toru Sugiyama,Atsumu Terada,Shin Nishio,Naotake Tsuda,Kiyoko Kato,Mayumi Ono,Michihiko Kuwano

Cell cycle (Georgetown, Tex.) 22:1496-1513 PubMed37245083

2023

TRIM13 inhibits cell proliferation and induces autophagy in lung adenocarcinoma by regulating KEAP1/NRF2 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Bo Yu,Yu Zhou,Jinxi He

Open medicine (Warsaw, Poland) 18:20230631 PubMed37009050

2023

A novel long noncoding RNA AC125257.1 facilitates colorectal cancer progression by targeting miR-133a-3p/CASC5 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanwen Liao,Zihan Zheng,Junye Liu,Jian Li,Rui Li,Shuqin Hu

Open medicine (Warsaw, Poland) 17:1623-1631 PubMed36329787

2022

SNRPB promotes cell cycle progression in thyroid carcinoma via inhibiting p53.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Deng,Xin Li,Wenlei Jiang,Jindan Tang

Bioengineered 13:2114-2129 PubMed35034547

2022

Activating transcription factor 3-activated long noncoding RNA forkhead box P4-antisense RNA 1 aggravates colorectal cancer progression by regulating microRNA-423-5p/nucleus accumbens associated 1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhouyang Cheng,Song Jiang,Ran Tao,Haipeng Ge,Jun Qin

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cyclin A1 antibody [EPR24373-146]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 antibody [EPR24373-146] (AB270940)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 antibody [EPR24373-146] (AB270940)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclin A1 antibody [EPR24373-146] (AB270940)

Western blot - Anti-Cyclin A1 antibody [EPR24373-146] (AB270940)

Western blot - Anti-Cyclin A1 antibody [EPR24373-146] (AB270940)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (7)

Product promise