Anti-Cyclin A2 antibody [E399]

• Flow Cyt

Lab

Flow Cytometry - Anti-Cyclin A2 antibody [E399] (AB32498)

Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling Cyclin A2 with Purified ab32498 at 1 : 30 dilution (10 μg/ml) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor™ 488, ab150077) secondary antibody was used at 1 : 2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabelled control - Cell without incubation with primary antibody and secondary antibody (Blue).

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Cyclin A2 antibody [E399] (AB32498)

Immunocytochemistry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Cyclin A2 with Purified ab32498 at 1/50 dilution (5.66 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

• WB

Supplier Data

Western blot - Anti-Cyclin A2 antibody [E399] (AB32498)

The smaller band is due to a tissue-specific splice variant(PMID 22745723). The band of 35kDa maybe the cleaved form(PMID : 12176996).

All lanes:

Western blot - Anti-Cyclin A2 antibody [E399] (ab32498) at 1/2000 dilution

Lane 1:

K-562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysate at 15 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

• WB

Supplier Data

Western blot - Anti-Cyclin A2 antibody [E399] (AB32498)

Cyclin A2 is down regulated at the G2/M phase.

All lanes:

Western blot - Anti-Cyclin A2 antibody [E399] (ab32498) at 1/1000 dilution

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate; Untreated, asynchronous cells (<a href='/ko/products/unavailable/hela-cell-cycle-lysates-thymidine-treated-nocodazole-treated-untreated-asynchronous-control-ab136811'>ab136811</a>) at 20 µg

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate; G1/S arrested cells (thymidine treatment) (<a href='/ko/products/unavailable/hela-cell-cycle-lysates-thymidine-treated-nocodazole-treated-untreated-asynchronous-control-ab136811'>ab136811</a>) at 20 µg

Lane 3:

HeLa (Human epithelial cell line from cervix adenocarcinoma) lysate; G2/M arrested cells (sequential thymidine and nocodazole treatments) (<a href='/ko/products/unavailable/hela-cell-cycle-lysates-thymidine-treated-nocodazole-treated-untreated-asynchronous-control-ab136811'>ab136811</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 49 kDa

Observed band size: 50 kDa

false

• WB

CiteAb

Western blot - Anti-Cyclin A2 antibody [E399] (AB32498)

Cyclin A2 western blot using anti-Cyclin A2 antibody [E399] ab32498. Publication image and figure legend from Wu, Z., Huang, X., et al., 2013, J Exp Clin Cancer Res, PubMed 24289849.

ab32498 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab32498 please see the product overview.

B-Myb acts as the downstream effector of mir-29a to regulate cell cycle. A, the scheme of the plasmid construction for the luciferase assay. B, relative luciferase activities of the cells (with or without mir-29a over-expression) transfected with either wild or mutant 3′-UTR of B-Myb; n = 5, Mean ± SD. C, protein levels of cyclin A2, cyclin D1 and B-Myb in MDA-MB-453 cells with or without mir-29a over-expression. D, protein levels of cyclin A2, cyclin D1 and B-Myb in MCF-10A cells with or without mir-29a knockdown.

false