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AB244991

Anti-Cyclin E1 antibody [EPR20777-118] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Cyclin E1 antibody. Carrier free. Suitable for WB, sELISA and reacts with Mouse, Human samples.
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Sandwich ELISA - Anti-Cyclin E1 antibody [EPR20777-118] - BSA and Azide free (Detector) (AB244991)
  • sELISA

Lab

Sandwich ELISA - Anti-Cyclin E1 antibody [EPR20777-118] - BSA and Azide free (Detector) (AB244991)

HCT 116 + Thymidine : HCT 116 treated first with 2 mM Thymidine for 18 hours, then recovered for 8 hours, and then treated again with 2 mM Thymidine for 18 hours, whole cell lysate. Serial dilutions of lysates were analyzed.
CCNE1 KO HCT 116 + Thymidine : CCNE1 knockout HCT 116 treated first with 2 mM Thymidine for 18 hours, then recovered for 8 hours, and then treated again with 2 mM Thymidine for 18 hours, whole cell lysate. Serial dilutions of lysates were analyzed.

Sandwich ELISA - Anti-Cyclin E1 antibody [EPR20777-118] - BSA and Azide free (Detector) (AB244991)
  • sELISA

Lab

Sandwich ELISA - Anti-Cyclin E1 antibody [EPR20777-118] - BSA and Azide free (Detector) (AB244991)

HCT 116 + Thymidine : HCT 116 treated first with 2 mM Thymidine for 18 hours, then recovered for 8 hours, and then treated again with 2 mM Thymidine for 18 hours, whole cell lysate. Serial dilutions of lysates were analyzed.
HCT 116 + Control : untreated HCT 116, whole cell lysate. Serial dilutions of lysates were analyzed.

Western blot - Anti-Cyclin E1 antibody [EPR20777-118] - BSA and Azide free (Detector) (AB244991)
  • WB

Supplier Data

Western blot - Anti-Cyclin E1 antibody [EPR20777-118] - BSA and Azide free (Detector) (AB244991)

Blocking Buffer and concentration : 5% NFDM/TBST

All lanes:

Western blot - Anti-Cyclin E1 antibody [EPR20777-118] - BSA and Azide free (Detector) (ab244991) at 1/1000 dilution

Lane 1:

MCF7 (Human breast adenocarcinoma epithelial cell) cell lysate at 10 µg

Lane 2:

HepG2 (Human hepatocellular carcinoma epithelial cell) cell lysate at 10 µg

Lane 3:

HT-29 (Human colorectal adenocarcinoma epithelial cell) cell lysate at 10 µg

Lane 4:

HAP1 (Human chronic myelogenous leukemia) cell lysate at 10 µg

Lane 5:

NIH/3T3 (Mouse embryonic fibroblast) cell lysate at 10 µg

Lane 6:

RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Exposure time: 1min

Sandwich ELISA - Anti-Cyclin E1 antibody [EPR20777-118] - BSA and Azide free (Detector) (AB244991)
  • sELISA

Lab

Sandwich ELISA - Anti-Cyclin E1 antibody [EPR20777-118] - BSA and Azide free (Detector) (AB244991)

Indirect ELISA analysis of ab326342 at 2000 ng/mL. The Secondary antibody used was Peroxidase AffiniPure Goat Anti-Rabbit IgG (Fc fragment specific) at 1 : 20000 dilution.

Antigen : Human Cyclin E1 (phospho T77) peptide; Human Cyclin E1 non-phospho peptide; Human Cyclin E2 (phospho T74) peptide.

Antigen concentration : 0.98-1000 ng/mL

Sandwich ELISA - Anti-Cyclin E1 antibody [EPR20777-118] - BSA and Azide free (Detector) (AB244991)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Cyclin E1 antibody [EPR20777-118] - BSA and Azide free (Detector) (AB244991)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab231929).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20777-118

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB231929

Human Cyclin E1 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Cyclin E1 ELISA Kit (AB231929)

  • 0
  • 0
Sample Prep & Detection Kits

AB269823

Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link®

Immunocytochemistry - Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (AB269823)

  • 5
  • 2
Sample Prep & Detection Kits

AB269902

Gold-Maleimide Conjugation Kit (20nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (20nm, 20 OD) (AB269902)

  • 0
  • 0
Proteins & Peptides

AB119719

Recombinant Human Cyclin E1 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Cyclin E1 protein (GST tag N-Terminus) (AB119719)

  • 0
  • 0
Primary Antibodies

AB279994

Anti-Cdk6 antibody [EPR24105-8] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Cdk6 antibody [EPR24105-8] - BSA and Azide free (Detector) (AB279994)

  • 0
  • 0
Assay Kits

AB289900

Human CDK6 ELISA Kit

SimpleStep

Sandwich ELISA - Human CDK6 ELISA Kit (AB289900)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab244991 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Cyclin E1. The recommended pair for sandwich ELISA is:
Capture: ab244724, Human Cyclin E1 Capture Antibody (unconjugated)
Detector: ab244991, Human Cyclin E1 Detector Antibody (unconjugated)
The reference range value is 3.91 - 250 ng/ml.

ab244991 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Cyclin E1 (phospho T77). The recommended pair for sandwich ELISA is:

Capture: ab326342, Human Cyclin E1 (phospho T77) Capture Antibody (unconjugated)

Detector: ab244991, Human Cyclin E1 (phospho T77) Detector Antibody (unconjugated)

The reference range value is 0.98-1000 ng/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Essential for the control of the cell cycle at the G1/S (start) transition.
See full target information CCNE1

대체 명칭 보기

CCNE, CCNE1, G1/S-specific cyclin-E1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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