Anti-CYP2S1 antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2S1 antibody (AB69650)

ab69650 at 1/50 dilution staining CYP2S1 in human lung carcinoma by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CYP2S1 antibody (AB69650)

ab69650 at 1/500 dilution staining CYP2S1 in A549 cells by Immunofluorescence, in the absence or presence of the immunising peptide

• WB

Unknown

Western blot - Anti-CYP2S1 antibody (AB69650)

All lanes:

Western blot - Anti-CYP2S1 antibody (ab69650) at 1/500 dilution

Lane 1:

HUVEC cell extract at 30 µg

Lane 2:

COLO cell extract at 30 µg

Lane 3:

COLO cell extract at 30 µg with immunising peptide

Predicted band size: 56 kDa

Observed band size: 56 kDa

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• WB

CiteAb

Western blot - Anti-CYP2S1 antibody (AB69650)

CYP2S1 western blot using anti-CYP2S1 antibody ab69650. Publication image and figure legend from D'Ambrosio, D. N., Walewski, J. L., et al., 2011, PLoS One, PubMed 21949825.

ab69650 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab69650 please see the product overview.

mRNA and protein expression of retinoid-metabolizing cytochrome P450 enzymes.(A) Relative mRNA expression levels of Cyp2s1 and Cyp2e1 in AF-HSCs (solid bars) and GFP-HSCs (stripped bars) are shown. Values are normalized to 18S and given as the mean ±1 S.D. for 5 AF and 5 GFP HSC isolations. Significance was determined by a Student's t-test, ** p-value<0.01. (B) Protein levels of CYP2S1 and CYP2E1 in AF-HSCs and GFP-HSCs. Experiments were repeated with similar results.

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