Anti-Cystatin SA/CST2 antibody [EPR25934-14]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin SA/CST2 antibody [EPR25934-14] (AB325126)

Immunohistochemical analysis of paraffin-embedded Human submandibular gland tissue labeling Cystatin SA/CST2 with ab325126 at 1/2000 (0.235 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human submandibular gland (PMID : 11879580).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Cystatin SA/CST2 antibody [EPR25934-14] (AB325126)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Cystatin SA/CST2 antibody [EPR25934-14] (ab325126) at 1/1000 dilution

All lanes:

HT-1197 (human urinary bladder carcinoma cell ) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 14 kDa,36 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Anti-Cystatin SA/CST2 antibody [EPR25934-14] (AB325126)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen, liver (PMID : 11879580).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Cystatin SA/CST2 antibody [EPR25934-14] (ab325126) at 1/1000 dilution

Lane 1:

Human salivary tissue lysate at 20 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 14 kDa,36 kDa

false

Exposure time: 1s

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin SA/CST2 antibody [EPR25934-14] (AB325126)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Cystatin SA/CST2 with ab325126 at 1/2000 (0.235 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human liver (PMID : 11879580).
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cystatin SA/CST2 antibody [EPR25934-14] (AB325126)

Immunohistochemical analysis of paraffin-embedded Human spleen tissue labeling Cystatin SA/CST2 with ab325126 at 1/2000 (0.235 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : No staining on human spleen.
The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-Cystatin SA/CST2 antibody [EPR25934-14] (AB325126)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human CST4 and CST1.

In Western blot, Anti-His tag® antibody (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-Cystatin SA/CST2 antibody [EPR25934-14] (ab325126) at 1/1000 dilution

Lane 1:

Untagged human Cystatin SA/CST2 recombinant protein at 10 ng

Lane 2:

His-tagged human CST4 recombinant protein at 10 ng

Lane 3:

His-tagged human CST1 recombinant protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 14 kDa,15-16 kDa

false

Exposure time: 15s