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AB316356

Anti-Cytochrome b245 Light Chain/p22-phox antibody [CS9]

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Mouse Monoclonal Cytochrome b245 Light Chain/p22-phox antibody. Suitable for Flow Cyt, ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Native Full Length Protein corresponding to Human Cytochrome b-245 light chain.

대체 명칭 보기

Cytochrome b-245 light chain, Cytochrome b(558) alpha chain, Cytochrome b558 subunit alpha, Neutrophil cytochrome b 22 kDa polypeptide, Superoxide-generating NADPH oxidase light chain subunit, p22 phagocyte B-cytochrome, p22-phox, p22phox, CYBA

5 이미지
Flow Cytometry - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)

Blue line : Flow cytometric analysis of MCF7 (Human breast adenocarcinoma cell line) cells. Primary incubation 1hr (1 : 100 dilution) followed by Alexa Fluor 488 ® conjugated goat Anti-mouse IgG (1 : 1000 dilution). Black line : Anti-Unknown Specificity Isotype control.

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)

Immunofluorescence analysis of paraformaldehyde fixed MCF7 (Human breast adenocarcinoma cell line) cells. Primary incubation 1hr (1 : 100 dilution) followed by Alexa Fluor® 488 secondary antibody (1 : 1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)

Immunofluorescence analysis of paraformaldehyde fixed MCF7 (Human breast adenocarcinoma cell line) cells. Primary incubation 1hr (1 : 100 dilution) followed by Alexa Fluor® 488 secondary antibody (1 : 1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.

Flow Cytometry - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)

Blue line : Flow cytometric analysis of MCF7 (Human breast adenocarcinoma cell line) cells. Primary incubation 1hr (1 : 100 dilution) followed by Alexa Fluor 488 ® conjugated goat Anti-mouse IgG (1 : 1000 dilution). Black line : Anti-Unknown Specificity Isotype control.

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytochrome b245 Light Chain/p22-phox Antibody [CS9] (AB316356)

Immunofluorescence analysis of paraformaldehyde fixed MCF7 (Human breast adenocarcinoma cell line) cells. Primary incubation 1hr (1 : 100 dilution) followed by Alexa Fluor® 488 secondary antibody (1 : 1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Mouse IgG1 negative control followed by Alexa Fluor® 488 secondary antibody.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CS9

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt

applications

Immunogen

Native Full Length Protein corresponding to Human Cytochrome b-245 light chain.

P13498

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보완 제품

Primary Antibodies

AB109523

Anti-NOXA2/p67phox antibody [EPR5065]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-NOXA2/p67phox antibody [EPR5065] (AB109523)

  • 0
  • 0
Primary Antibodies

AB310337

Anti-NOX2/gp91phox antibody [EPR28415-13]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-NOX2/gp91phox antibody [EPR28415-13] (AB310337)

  • 0
  • 0
Primary Antibodies

AB166930

Anti-NCF1/p47-phox antibody

Immunocytochemistry/ Immunofluorescence - Anti-NCF1/p47-phox antibody (AB166930)

  • 5
  • 2
Primary Antibodies

AB68523

Anti-NOXA1 antibody

  • 5
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1 µg/mL", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
보관 버퍼
Preservative: 0.1% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1 month
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Cytochrome b245 Light Chain also known as p22-phox or CYBA plays a significant role in the NADPH oxidase complex. This protein has a molecular mass of approximately 22 kDa and is widely expressed in various cell types particularly in those associated with the immune system such as neutrophils. p22-phox forms part of the membrane-bound flavocytochrome b558 along with gp91-phox which is important for the generation of reactive oxygen species.
Biological function summary

The p22-phox serves as a core component of the NADPH oxidase complex which is integral to cellular responses to infection and stress. It operates in conjunction with other cytosolic subunits to produce reactive oxygen species that help in microbial destruction and signal transduction. The presence of p22-phox is essential for proper enzyme assembly and function as its interaction with gp91-phox establishes the electron transport chain required for oxidase activity.

Pathways

P22-phox contributes to the oxidative burst pathway a critical mechanism in innate immunity. In this pathway its interactions with proteins like Rac a small GTPase facilitate the activation and membrane association of the oxidase complex. Additionally p22-phox has involvement in inflammatory pathways by aiding in the release of signaling molecules that trigger inflammatory responses. This protein cooperates with components of the MAPK signaling pathway acting as a mediator in signal transduction events that dictate cellular responses to external stimuli.

P22-phox links closely with chronic granulomatous disease (CGD) a disorder characterized by a malfunctioning NADPH oxidase complex. Mutations in the CYBA gene can lead to defects in reactive oxygen species generation compromising the immune system's ability to combat infections. Furthermore evidence suggests a connection between p22-phox and cardiovascular diseases including hypertension where abnormalities in oxidase activity influence endothelial function. Proteins such as gp91-phox also share these disease associations highlighting the cooperative interaction of these components in disease pathology.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Subunit of NADPH oxidase complexes that is required for the NADPH oxidase activity that generates, in various cell types, superoxide from molecular oxygen utilizing NADPH as an electron donor (PubMed : 15824103, PubMed : 17140397, PubMed : 38355798). Subunit of the phagocyte NADPH oxidase complex that mediates the transfer of electrons from cytosolic NADPH to O2 to produce the superoxide anion (O2(-)) (PubMed : 38355798). In the activated complex, electrons are first transferred from NADPH to flavin adenine dinucleotide (FAD) and subsequently transferred via two heme molecules to molecular oxygen, producing superoxide through an outer-sphere reaction (PubMed : 38355798). Activation of the NADPH oxidase complex is initiated by the assembly of cytosolic subunits of the NADPH oxidase complex with the core NADPH oxidase complex to form a complex at the plasma membrane or phagosomal membrane (PubMed : 38355798). This activation process is initiated by phosphorylation dependent binding of the cytosolic NCF1/p47-phox subunit to the C-terminus of CYBA/p22-phox (PubMed : 19948736). Aassociates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide (PubMed : 15824103, PubMed : 17140397).
See full target information Cytochrome b-245 light chain
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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