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AB236343

Anti-Cytochrome P450 2E1 antibody [M12P4H2] - BSA and Azide free

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Mouse Monoclonal Cytochrome P450 2E1 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CYP2E1 aa 450 to C-terminus conjugated to Ovalbumin.

대체 명칭 보기

CYP2E, CYP2E1, Cytochrome P450 2E1, 4-nitrophenol 2-hydroxylase, CYPIIE1, Cytochrome P450-J

1 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 2E1 antibody [M12P4H2] - BSA and Azide free (AB236343)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 2E1 antibody [M12P4H2] - BSA and Azide free (AB236343)

Formalin-fixed, paraffin-embedded human hepatic carcinoma tissue stained for Cytochrome P450 2E1 using ab234408 at 2 μg/ml in immunohistochemical analysis.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab234408).

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

M12P4H2

Isotype

IgG1

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human CYP2E1 aa 450 to C-terminus conjugated to Ovalbumin. The exact immunogen used to generate this antibody is proprietary information.

P05181

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대체품

Primary Antibodies

AB234408

Anti-Cytochrome P450 2E1 antibody [M12P4H2]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 2E1 antibody [M12P4H2] (AB234408)

  • 0
  • 0
Primary Antibodies

AB28146

Anti-Cytochrome P450 2E1 antibody

Western blot - Anti-Cytochrome P450 2E1 antibody (AB28146)

  • 5
  • 6
Primary Antibodies

AB53945

Anti-Cytochrome P450 2E1 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytochrome P450 2E1 antibody (AB53945)

  • 0
  • 0

보완 제품

Primary Antibodies

AB135372

Anti-CD3 antibody [SP162]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD3 antibody [SP162] (AB135372)

  • 4
  • 10
Primary Antibodies

AB76020

Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sodium Potassium ATPase antibody [EP1845Y] - Plasma Membrane Loading Control (AB76020)

  • 5
  • 18
Assay Kits

AB228553

Nile Red Staining Kit

Fluorescence Microscopy - Nile Red Staining Kit (AB228553)

  • 0
  • 0
Primary Antibodies

AB181598

Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker

20ul selling size|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 7 antibody [EPR17078] - Cytoskeleton Marker (AB181598)

  • 5
  • 13
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>Incubate with primary antibody for 30 minutes at room temperature.</p>" }, "Cynomolgus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rhesus monkey": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
Purification 관련 사항
Purified from bioreactor concentrate by Protein A/G.
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cytochrome P450 2E1 also known as CYP2E1 is an enzyme belonging to the cytochrome P450 superfamily. It weighs approximately 56 kDa. This enzyme is majorly expressed in the liver but can also be found in other tissues like the kidney and lungs. It plays a central role in the oxidation and metabolism of various endogenous and exogenous substances including fatty acids drugs and alcohols.
Biological function summary

The enzyme CYP2E1 metabolizes low-molecular-weight compounds. It catalyzes the conversion of substrates to more soluble forms enabling their excretion from the body. It is not a part of a larger protein complex but often partners with NADPH-cytochrome P450 reductase in electron transfer. CYP2E1 can also produce reactive oxygen species during its catalytic cycle which may impact cellular structures and processes.

Pathways

CYP2E1 is involved in the oxidative metabolism of ethanol and other xenobiotics. It participates in the metabolic pathways for drugs and carcinogens transforming them into either active or inactive metabolites. The enzyme has connections with other P450 enzymes such as CYP3A4 within the same metabolic landscape often overlapping substrates and regulatory mechanisms.

CYP2E1 plays a significant role in alcohol-induced liver injury because its metabolism of ethanol generates toxic by-products like acetaldehyde and reactive oxygen species contributing to liver damage. The enzyme is also associated with the development of certain cancers as its metabolism of procarcinogens can produce carcinogenic metabolites. Other related cytochrome P450 enzymes such as CYP1A2 share pathways involved in carcinogen activation highlighting an interconnected network contributing to disease progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

A cytochrome P450 monooxygenase involved in the metabolism of fatty acids (PubMed : 10553002, PubMed : 18577768). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase) (PubMed : 10553002, PubMed : 18577768). Catalyzes the hydroxylation of carbon-hydrogen bonds. Hydroxylates fatty acids specifically at the omega-1 position displaying the highest catalytic activity for saturated fatty acids (PubMed : 10553002, PubMed : 18577768). May be involved in the oxidative metabolism of xenobiotics (Probable).
See full target information CYP2E1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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