AB249049

Anti-Cytohesin 1 antibody [EPR10051] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Cytohesin 1 antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human samples.

대체 명칭 보기

D17S811E, PSCD1, CYTH1, Cytohesin-1, SEC7 homolog B2-1

2 이미지

Flow Cytometry (Intracellular) - Anti-Cytohesin 1 antibody [EPR10051] - BSA and Azide free (AB249049)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cytohesin 1 antibody [EPR10051] - BSA and Azide free (AB249049)

This data was developed using ab151732, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized HUT78 cells labeling Cytohesin 1 using ab151732 at a 1/100 dilution (red) or a rabbit IgG control (negative) (green).

Unknown

Western blot - Anti-Cytohesin 1 antibody [EPR10051] - BSA and Azide free (AB249049)

This data was developed using ab151732, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cytohesin 1 antibody [EPR10051] (<a href='/ko/products/primary-antibodies/cytohesin-1-antibody-epr10051-ab151732'>ab151732</a>) at 1/1000 dilution

Lane 1:

Jurkat cell lysates

Lane 2:

Raji cell lysates

Lane 3:

K562 cell lysates

Lane 4:

HUT78 cell lysates

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 46 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10051

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

ab249049 is the carrier-free version of ab151732.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cytohesin 1 also known as CYTH1 or PSCD1 belongs to the cytohesin family. It plays a role as a guanine nucleotide exchange factor (GEF) specifically for Arf (ADP-ribosylation factor) proteins. Cytohesin 1 weighs approximately 47 kDa. It contributes to cellular communication and membrane trafficking activities by activating Arf family GTPases. You can find it widely expressed in tissues including immune cells and the nervous system. It localizes to cytoplasmic regions and associates with the plasma membrane through a pleckstrin homology (PH) domain.

Biological function summary

Cytohesin 1 influences multiple cellular processes like actin cytoskeleton remodeling cell migration and adhesion. It directs signal transduction pathways that regulate these key processes contributing to how cells interact with their external environment. Although Cytohesin 1 can function alone it typically operates as part of protein complexes that include partners like ARNO (another GEF for Arf proteins) that fine-tune intracellular signaling dynamics. This protein also interacts with other signaling molecules through its Sec7 and PH domains expanding its role across various cellular contexts.

Pathways

Cytohesin 1 integrates into significant signaling cascades such as the Arf6 pathway and the phosphoinositide 3-kinase (PI3K) pathway. Through these pathways it compounds cellular responses related to vesicular trafficking and cell morphology adjustments. In the Arf6 pathway Cytohesin 1 acts alongside proteins such as Arf6 and EFA6 to modulate membrane dynamics essential for endocytosis and stress fiber formation. In the PI3K pathway it collaborates with signaling molecules to regulate growth factor-mediated responses highlighting the interconnectivity Cytohesin 1 maintains between various pathway components.

Cytohesin 1 has been linked to immune-related diseases and neurological disorders. In the context of the immune system it plays a role in T cell activation and inflammation and alterations in its functioning may contribute to conditions like autoimmunity. In neurological contexts Cytohesin 1 interacts with proteins related to cellular adhesion and neurite outgrowth which can impact neurological functions and may play a role in developmental disorders. Its relationship with proteins like integrins also suggests how its dysregulation can affect cell attachment and signaling impacting disease progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6. Promotes the activation of ARF factors through replacement of GDP with GTP. Plays an important role in membrane trafficking, during junctional remodeling and epithelial polarization, through regulation of ARF6 activity.

See full target information CYTH1

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytohesin 1 antibody [EPR10051] - BSA and Azide free

Flow Cytometry (Intracellular) - Anti-Cytohesin 1 antibody [EPR10051] - BSA and Azide free (AB249049)

Western blot - Anti-Cytohesin 1 antibody [EPR10051] - BSA and Azide free (AB249049)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Product promise