AB9220

Anti-Cytokeratin 14 antibody [RCK107]

Name: Anti-Cytokeratin 14 antibody [RCK107]
Brand: Abcam Limited
SKU: AB9220
Rating: 3 (3 reviews)

(3 리뷰들)

(41 제품이 사용된 논문 )

Mouse Monoclonal Cytokeratin 14 antibody. Suitable for WB, ICC/IF, IHC-Fr and reacts with Human, Pig, Dog samples. Cited in 41 publications. Immunogen corresponding to Cell preparation containing TR146 cell line protein.

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Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [RCK107] (AB9220)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [RCK107] (AB9220)

ICC/IF image of ab9220 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab9220, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (AB9220)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (AB9220)

Immunohistochemical analysis of dog skin epidermis tissue labelling Cytokeratin 14 using ab9220. Staining of basal keratinocyte layer is observed.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (AB9220)

Immunohistochemical analysis of swine skin tissue labeling Cytokeratin 14 with ab9220. Showing staining of basal cells.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (AB9220)

Immunohistochemical analysis of dog skin appendages labelling Cytokeratin 14 with ab9220.

Unknown

Western blot - Anti-Cytokeratin 14 antibody [RCK107] (AB9220)

All lanes:

Western blot - Anti-Cytokeratin 14 antibody [RCK107] (ab9220) at 4 µg/mL

All lanes:

Human skin tissue lysate - total protein (<a href='/ko/products/unavailable/human-skin-tissue-lysate-total-protein-ab30166'>ab30166</a>) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/5000 dilution

Predicted band size: 52 kDa

Observed band size: 58 kDa,72 kDa

true

Exposure time: 20min

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

RCK107

Isotype

IgG1

Carrier free

Reacts with

Dog, Human, Pig

Applications

ICC/IF, WB, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts exclusively with cytokeratin 14 which is present in basal cell compartments of stratified and combined epithelia.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Dog": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/200", "IHCFr-species-notes": "" }, "Pig": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100 - 1/200", "IHCFr-species-notes": "" } } }

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제품 세부 정보

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology assay.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.09% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 14 also known as CK14 keratin 14 or KRT14 is a type I keratin protein with a molecular mass of approximately 50 kDa. It forms intermediate filaments in epithelial cells primarily in the basal layer of stratified epithelium and is part of the cytoskeleton. Cytokeratin 14 expression appears in tissues such as the epidermis tongue and esophagus where it contributes to the structural integrity and mechanical stability of cells. Laboratories often utilize antibodies like anti-K14 and labels such as Alexa Fluor 555 or Alexa Fluor 647 to identify cytokeratin 14 in research settings.

Biological function summary

This protein significantly contributes to the maintenance and regeneration of epithelial tissues by forming a network of filaments with keratin 5. KRT14 pairs with keratin 5 to create a keratin intermediate filament complex which plays a structural role in the resilience and elasticity of epithelial tissues. This partnership is essential for the assembly of the cytoskeleton and supports the protective barrier function of the skin and related tissues.

Pathways

Cytokeratin 14 engages in pivotal cellular processes like keratinization and wound healing. The protein is actively involved in signal transduction pathways such as the epithelial cell differentiation pathway. Within these pathways KRT14 collaborates with KRT5 and other structural proteins to regulate cell proliferation and differentiation reflecting its importance in skin health and function.

Cytokeratin 14 associates with specific genetic conditions including epidermolysis bullosa simplex (EBS) and squamous cell carcinoma (SCC). In EBS mutations in the KRT14 gene lead to skin fragility. In SCC altered expression of KRT14 and related proteins such as keratin 5 can influence tumor progression and invasion. These associations highlight the critical role of KRT14 in both normal and pathological epithelial function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

See full target information Keratin, type I cytoskeletal 14

대체 명칭 보기

Cytokeratin-14, Keratin-14, CK-14, K14, KRT14

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제품이 사용된 논문 (41)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Animals : an open access journal from MDPI 14: PubMed39123758

2024

Mechanobiological Strategies to Enhance Ovine () Adipose-Derived Stem Cells Tendon Plasticity for Regenerative Medicine and Tissue Engineering Applications.

Applications

Unspecified application

Species

Unspecified reactive species

Arlette A Haidar-Montes,Annunziata Mauro,Mohammad El Khatib,Giuseppe Prencipe,Laura Pierdomenico,Umberto Tosi,Guy Wouters,Adrián Cerveró-Varona,Paolo Berardinelli,Valentina Russo,Barbara Barboni

Bioengineering & translational medicine 9:e10640 PubMed39036091

2024

Highly efficient CRISPR/Cas9-mediated exon skipping for recessive dystrophic epidermolysis bullosa.

Applications

Unspecified application

Species

Unspecified reactive species

Alex du Rand,John Hunt,Christopher Samson,Evert Loef,Chloe Malhi,Sarah Meidinger,Chun-Jen Jennifer Chen,Ashley Nutsford,John Taylor,Rod Dunbar,Diana Purvis,Vaughan Feisst,Hilary Sheppard

International journal of molecular sciences 25: PubMed38255836

2024

Splicing Modulation via Antisense Oligonucleotides in Recessive Dystrophic Epidermolysis Bullosa.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Hainzl,Lisa Trattner,Bernadette Liemberger,Johannes Bischof,Thomas Kocher,Michael Ablinger,Alexander Nyström,Astrid Obermayer,Alfred Klausegger,Christina Guttmann-Gruber,Verena Wally,Johann W Bauer,Josefina Piñón Hofbauer,Ulrich Koller

Burns & trauma 10:tkac023 PubMed36225328

2022

WNT5A drives interleukin-6-dependent epithelial-mesenchymal transition via the JAK/STAT pathway in keloid pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Young In Lee,Jung Eun Shim,Jihee Kim,Won Jai Lee,Jae Woo Kim,Kee Hyun Nam,Ju Hee Lee

Cell reports. Medicine 3:100656 PubMed35732145

2022

Filamentous bacteriophage delays healing of Pseudomonas-infected wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Michelle S Bach,Christiaan R de Vries,Arya Khosravi,Johanna M Sweere,Medeea C Popescu,Qingquan Chen,Sally Demirdjian,Aviv Hargil,Jonas D Van Belleghem,Gernot Kaber,Maryam Hajfathalian,Elizabeth B Burgener,Dan Liu,Quynh-Lam Tran,Tejas Dharmaraj,Maria Birukova,Vivekananda Sunkari,Swathi Balaji,Nandini Ghosh,Shomita S Mathew-Steiner,Mohamed S El Masry,Sundeep G Keswani,Niaz Banaei,Laurence Nedelec,Chandan K Sen,Venita Chandra,Patrick R Secor,Gina A Suh,Paul L Bollyky

JID innovations : skin science from molecules to population health 2:100139 PubMed36090299

2022

RAB3A Regulates Melanin Exocytosis and Transfer Induced by Keratinocyte-Conditioned Medium.

Applications

Unspecified application

Species

Unspecified reactive species

Luís C Cabaço,Liliana Bento-Lopes,Matilde V Neto,Andreia Ferreira,Wanja B L Staubli,José S Ramalho,Miguel C Seabra,Duarte C Barral

Molecular therapy : the journal of the American Society of Gene Therapy 30:2680-2692 PubMed35490295

2022

Paired nicking-mediated COL17A1 reframing for junctional epidermolysis bullosa.

Applications

Unspecified application

Species

Unspecified reactive species

Johannes Bischof,Oliver Patrick March,Bernadette Liemberger,Simone Alexandra Haas,Stefan Hainzl,Igor Petković,Victoria Leb-Reichl,Julia Illmer,Evgeniia Korotchenko,Alfred Klausegger,Anna Hoog,Heide-Marie Binder,Marta Garcia,Blanca Duarte,Dirk Strunk,Fernando Larcher,Julia Reichelt,Christina Guttmann-Gruber,Verena Wally,Josefina Piñón Hofbauer,Johann Wolfgang Bauer,Toni Cathomen,Thomas Kocher,Ulrich Koller

iScience 25:103982 PubMed35310332

2022

Dormant reporter-marked basal stem/progenitor cells contribute to mammary lobuloalveoli formation.

Applications

Unspecified application

Species

Unspecified reactive species

Ruiqi Liu,Huan Hu,Melissa McNeil,Jiuzhi Xu,Xueyun Bi,Pengbo Lou,Christian F Guerrero-Juarez,Xing Dai,Maksim V Plikus,Jianwei Shuai,Zhengquan Yu,Cong Lv

International neurourology journal 26:S57-67 PubMed35073671

2022

Improved Urothelial Cell Proliferation, Cytoskeleton and Barrier Function Protein Expression in the Patients With Interstitial Cystitis/Bladder Pain Syndrome After Intravesical Platelet-Rich Plasma Injection.

Applications

Unspecified application

Species

Unspecified reactive species

Jia-Fong Jhang,Yuan-Hong Jiang,Yung-Hsiang Hsu,Han-Chen Ho,Lori A Birder,Teng-Yi Lin,Hann-Chorng Kuo

Biomedicines 10: PubMed35203430

2022

Inflammation and Barrier Function Deficits in the Bladder Urothelium of Patients with Chronic Spinal Cord Injury and Recurrent Urinary Tract Infections.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Yu Wu,Yuan-Hong Jiang,Jia-Fong Jhang,Yung-Hsiang Hsu,Han-Chen Ho,Hann-Chorng Kuo

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 14 antibody [RCK107]

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 14 antibody [RCK107] (AB9220)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (AB9220)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (AB9220)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 14 antibody [RCK107] (AB9220)

Western blot - Anti-Cytokeratin 14 antibody [RCK107] (AB9220)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (41)

Product promise