AB233910

Anti-Cytokeratin 14 antibody [SPM263] - C-terminal

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Mouse Monoclonal Cytokeratin 14 antibody. C-terminal. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human Keratin, type I cytoskeletal 14.

대체 명칭 보기

Cytokeratin-14, Keratin-14, CK-14, K14, KRT14

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [SPM263] - C-terminal (AB233910)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [SPM263] - C-terminal (AB233910)

Formalin-fixed, paraffin-embedded human prostate carcinoma tissue stained for Cytokeratin 14 using ab233910 at 1 μg/ml in immunohistochemical analysis.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

SPM263

Isotype

IgG3

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human Keratin, type I cytoskeletal 14. The exact immunogen used to generate this antibody is proprietary information.

P02533

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대체품

Primary Antibodies

AB192055

Alexa Fluor® 488 Anti-Cytokeratin 14 antibody [EP1612Y]

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-Cytokeratin 14 antibody [EP1612Y] (AB192055)

Primary Antibodies

AB192056

Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EP1612Y]

RabMAb|Recombinant|KO Validated

Immunohistochemistry - Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EP1612Y] (AB192056)

Primary Antibodies

AB206100

Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-Cytokeratin 14 antibody [EPR17350] (AB206100)

Primary Antibodies

AB181595

Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - Cytoskeleton Marker (AB181595)

Primary Antibodies

AB51054

Anti-Cytokeratin 14 antibody [EP1612Y]

20ul selling size|RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Cytokeratin 14 antibody [EP1612Y] (AB51054)

Primary Antibodies

AB220818

Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free

BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17350] - BSA and Azide free (AB220818)

Primary Antibodies

AB197893

Anti-Cytokeratin 14 antibody [EPR17336]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 14 antibody [EPR17336] (AB197893)

보완 제품

Assay Kits

AB226895

Human Cytokeratin 14 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Cytokeratin 14 ELISA Kit (AB226895)

Primary Antibodies

AB16667

Anti-Ki67 antibody [SP6]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [SP6] (AB16667)

Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

Primary Antibodies

AB52866

Anti-alpha Tubulin antibody [EP1332Y] - Loading Control

Primary Antibodies

AB52635

Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A/G

Purification 관련 사항

ab233910 purified from bioreactor concentrate by Protein A/G.

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 14 also known as CK14 keratin 14 or KRT14 is a type I keratin protein with a molecular mass of approximately 50 kDa. It forms intermediate filaments in epithelial cells primarily in the basal layer of stratified epithelium and is part of the cytoskeleton. Cytokeratin 14 expression appears in tissues such as the epidermis tongue and esophagus where it contributes to the structural integrity and mechanical stability of cells. Laboratories often utilize antibodies like anti-K14 and labels such as Alexa Fluor 555 or Alexa Fluor 647 to identify cytokeratin 14 in research settings.

Biological function summary

This protein significantly contributes to the maintenance and regeneration of epithelial tissues by forming a network of filaments with keratin 5. KRT14 pairs with keratin 5 to create a keratin intermediate filament complex which plays a structural role in the resilience and elasticity of epithelial tissues. This partnership is essential for the assembly of the cytoskeleton and supports the protective barrier function of the skin and related tissues.

Pathways

Cytokeratin 14 engages in pivotal cellular processes like keratinization and wound healing. The protein is actively involved in signal transduction pathways such as the epithelial cell differentiation pathway. Within these pathways KRT14 collaborates with KRT5 and other structural proteins to regulate cell proliferation and differentiation reflecting its importance in skin health and function.

Cytokeratin 14 associates with specific genetic conditions including epidermolysis bullosa simplex (EBS) and squamous cell carcinoma (SCC). In EBS mutations in the KRT14 gene lead to skin fragility. In SCC altered expression of KRT14 and related proteins such as keratin 5 can influence tumor progression and invasion. These associations highlight the critical role of KRT14 in both normal and pathological epithelial function.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

The nonhelical tail domain is involved in promoting KRT5-KRT14 filaments to self-organize into large bundles and enhances the mechanical properties involved in resilience of keratin intermediate filaments in vitro.

See full target information Keratin, type I cytoskeletal 14

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

JCI insight 5: PubMed32853177

2020

Contribution of plasma cells and B cells to hidradenitis suppurativa pathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Johann E Gudjonsson,Lam C Tsoi,Feiyang Ma,Allison C Billi,K R van Straalen,A R J V Vossen,H H van der Zee,Paul W Harms,Rachael Wasikowski,Christine M Yee,Syed M Rizvi,Xianying Xing,Enze Xing,Olesya Plazyo,Chang Zeng,Matthew T Patrick,Margaret M Lowe,Richard E Burney,Jeffrey H Kozlow,Jill R Cherry-Bukowiec,Yanyun Jiang,Joseph Kirma,Stephan Weidinger,Kelly C Cushing,Michael D Rosenblum,Celine Berthier,Amanda S MacLeod,John J Voorhees,Fei Wen,J Michelle Kahlenberg,Emanual Maverakis,Robert L Modlin,Errol P Prens

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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