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AB212566

Anti-Cytokeratin 19 antibody [A53-B/A2.26] - BSA and Azide free

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Mouse Monoclonal Cytokeratin 19 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing KRT19 protein.

대체 명칭 보기

Cytokeratin-19, Keratin-19, CK-19, K19, KRT19, ck19, ck 19

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [A53-B/A2.26] - BSA and Azide free (AB212566)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 19 antibody [A53-B/A2.26] - BSA and Azide free (AB212566)

Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Cytokeratin 19 using ab212566 at 1 μg/ml in immunohistochemical analysis.

This IHC-P data was generated using the same anti-Cytokeratin 19 antibody clone, A53-B/A2.26, in a different buffer formulation.

Western blot - Anti-Cytokeratin 19 antibody [A53-B/A2.26] - BSA and Azide free (AB212566)
  • WB

Supplier Data

Western blot - Anti-Cytokeratin 19 antibody [A53-B/A2.26] - BSA and Azide free (AB212566)

This IHC-P data was generated using the same anti-Cytokeratin 19 antibody clone, A53-B/A2.26, in a different buffer formulation.

All lanes:

Western blot - Anti-Cytokeratin 19 antibody [A53-B/A2.26] - BSA and Azide free (ab212566)

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Lane 2:

MCF7 (human breast adenocarcinoma cell line) cell lysate

Predicted band size: 44 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

A53-B/A2.26

Isotype

IgG2a

Light chain 타입

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Cell preparation containing KRT19 protein. The exact immunogen used to generate this antibody is proprietary information.

P08727

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 min at RT).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 min at RT).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" }, "Ferret": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p>(Primary incubation for 30 min at RT).</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
Purification 관련 사항
ab212566 was purified from Bioreactor Concentrate by Protein A/G.
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 19 (CK19) also known as cytokera­tine 19 keratin 19 (KRT19) or CK19 is a type of intermediate filament found in epithelial cells. It serves as an essential component of the cytoskeleton. CK19 weighs approximately 40 kDa and is broadly expressed in simple epithelial tissues including liver intestine and various glandular epithelial cells. As a cytokeratin marker it often appears in combination with others like cytokeratin 17 in certain tissue types helping to maintain the structural integrity of cells.
Biological function summary

CK19 plays a role in maintaining the cytoskeletal framework that provides mechanical support and helps control cell size and shape. It is not part of a large protein complex but works closely with other cytokeratins to ensure the stability and resilience of epithelial tissues. The A53-B/A22.26 positive staining is often used to confirm its presence in clinical histopathology.

Pathways

CK19 connects to cellular processes especially involving the keratinization and epithelial cell differentiation pathways. It interacts with other cytokeratins including BA16 and KR17 to facilitate these processes. This interaction aids in modulating the dynamics of cytoskeletal rearrangement during cell movement division and differentiation.

CK19 overexpression is linked with certain cancers notably hepatocellular carcinoma and breast cancer. It can serve as a prognostic marker in these diseases often in conjunction with other markers like CK17. The involvement of CK19 in the epithelial-mesenchymal transition (EMT) process connects it to the progression and aggressiveness of tumors facilitated by interactions with proteins involved in EMT pathways.

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
See full target information KRT19

제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 12:4906 PubMed34385456

2021

An N-Cadherin 2 expressing epithelial cell subpopulation predicts response to surgery, chemotherapy and immunotherapy in bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kenneth H Gouin,Nathan Ing,Jasmine T Plummer,Charles J Rosser,Bassem Ben Cheikh,Catherine Oh,Stephanie S Chen,Keith Syson Chan,Hideki Furuya,Warren G Tourtellotte,Simon R V Knott,Dan Theodorescu
제품이 사용된 논문 모두 보기

Product promise

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