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AB236751

Anti-Cytokeratin 2 antibody

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Rabbit Polyclonal Cytokeratin 2P antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human KRT76 aa 150-300.

대체 명칭 보기

KRT2B, KRT2P, KRT76, Cytokeratin-2P, Keratin-76, Type-II keratin Kb9, CK-2P, K2P, K76

2 이미지
Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 2 antibody (AB236751)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 2 antibody (AB236751)

4% formaldehyde-fixed, 0.2% Triton X-100 permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cells labeling Cytokeratin 2 (green) with ab236751 at 1/133 dilution in ICC/IF. Counter-stained with DAPI. The secondary antibody was Alexa Fluor® 488-congugated Goat Anti-Rabbit IgG (H+L).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2 antibody (AB236751)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2 antibody (AB236751)

Paraffin-embedded human endometrial cancer tissue stained for Cytokeratin 2 using ab236751 at 1/400 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human KRT76 aa 150-300. The exact immunogen used to generate this antibody is proprietary information.

Q01546

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대체품

Primary Antibodies

AB16111

Anti-Cytokeratin 2 antibody [AE3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2 antibody [AE3] (AB16111)

  • 0
  • 0

보완 제품

Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
Primary Antibodies

AB92547

Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Vimentin antibody [EPR3776] - Cytoskeleton Marker (AB92547)

  • 5
  • 92
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB16669

Anti-CD3 epsilon antibody [SP7]

BOND RX™ Validated|RabMAb|Advanced Validation|Recombinant|Lab Essentials|20ul selling size

Multiplex immunohistochemistry - Anti-CD3 epsilon antibody [SP7] (AB16669)

  • 4
  • 80
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 2 also known as KRT2 is a type II cytokeratin with a molecular weight of approximately 66 kDa. This protein is primarily expressed in the suprabasal layers of the epidermis especially in the upper spinous and granular layers of the skin. KRT2 plays a role in forming and maintaining the structural integrity of the epithelial cells due to its ability to assemble into intermediate filaments that provide mechanical support and resilience to epithelial tissues.
Biological function summary

Cytokeratin 2 contributes to epidermal differentiation and the formation of the skin barrier. It acts as part of the cytoskeletal network in keratinocytes providing structural support to the cells. Cytokeratin 2 is often found operating together with other keratin proteins as part of a complex. Its filamentous structure helps resist stress and external forces allowing the skin to withstand physical and chemical insults.

Pathways

Cytokeratin 2 has a significant role in keratinocyte differentiation and the skin barrier formation pathways. It is intricately related to other members of the keratin family such as Cytokeratin 1 (KRT1) which further regulate these processes. Cytokeratin 2 expression affects pathways that control cell proliferation and apoptosis within the skin ensuring the balance between cell division and cell death necessary for maintaining healthy skin tissue.

Deficiencies or mutations in Cytokeratin 2 can lead to Ichthyosis Bullosa of Siemens a condition characterized by blistering and peeling skin. Mutations may affect the stability and assembly of keratin filaments compromising skin integrity. Cytokeratin 2 abnormalities connect with other cytokeratin proteins like KRT1 which also contribute to similar skin disorders. Understanding its role and interactions with other proteins may assist in the development of treatments for keratin-associated skin diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Probably contributes to terminal cornification.
See full target information KRT76
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of experimental & clinical cancer research : CR 43:68 PubMed38439082

2024

MUC20 regulated by extrachromosomal circular DNA attenuates proteasome inhibitor resistance of multiple myeloma by modulating cuproptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobin Wang,Yingqing Shi,Hua Shi,Xiaoyu Liu,Aijun Liao,Zhuogang Liu,Robert Z Orlowski,Rui Zhang,Huihan Wang
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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