AB249533

Anti-Cytokeratin 2e antibody [EPR10911] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal K22E antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

KRT2A, KRT2E, KRT2, Cytokeratin-2e, Epithelial keratin-2e, Keratin-2 epidermis, Keratin-2e, Type-II keratin Kb2, CK-2e, K2e

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody [EPR10911] - BSA and Azide free (AB249533)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody [EPR10911] - BSA and Azide free (AB249533)

This data was developed using ab170106, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin embedded Human skin tissue labeling Cytokeratin 2e with ab170106 at a 1/100 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Unknown

Western blot - Anti-Cytokeratin 2e antibody [EPR10911] - BSA and Azide free (AB249533)

This data was developed using ab170106, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Cytokeratin 2e antibody [EPR10911] (<a href='/ko/products/primary-antibodies/cytokeratin-2e-antibody-epr10911-ab170106'>ab170106</a>) at 1/1000 dilution

Lane 1:

A431 cell lysate at 10 µg

Lane 2:

HACAT cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 65 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10911

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

ab249533 is the carrier-free version of ab170106.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Cytokeratin 2e also known as keratin 2 or KRT2 is a type of intermediate filament protein with a mass of approximately 65 kDa. It functions mechanically to form and maintain structural integrity in epithelial cells. Cytokeratin 2e forms part of the keratin cytoskeleton which gives cells shape support and resilience against mechanical stress. This protein is expressed mainly in the upper spinous layer of the epidermis where it contributes to the specialized cytokeratin network.

Biological function summary

Cytokeratin 2e participates in cell differentiation and maturation within the epidermis. It does not operate in isolation but is integrated into a complex network of filaments comprising various keratin isoforms. This structured network assists in the development of the skin barrier which is essential for protecting the body from environmental damage. By facilitating the cohesion between keratinocytes Cytokeratin 2e plays a role in maintaining proper epidermal function.

Pathways

Cytokeratin 2e involves the epidermal differentiation pathway. It interacts primarily with other keratin proteins like keratin 1 (KRT1) to enhance the formation of a robust keratinocyte cytoskeleton. This association ensures proper skin cell function and epidermis homeostasis. The protein is also involved in the cornification pathway helping the process whereby keratinocytes become corneocytes the cells that make up the skin's outermost layer.

Cytokeratin 2e has links to ichthyosis bullosa of Siemens a keratinization disorder. Mutations affecting Cytokeratin 2e disrupt the structural stability of keratin filaments leading to skin fragility and blistering. Additionally its aberrant expression has associations with psoriasis where it may interact with immune-modulatory proteins like interleukin-17 (IL-17) aggravating the inflammatory environment. Understanding these connections helps in exploring therapeutic targets for managing these skin conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Probably contributes to terminal cornification (PubMed : 1380918). Associated with keratinocyte activation, proliferation and keratinization (PubMed : 12598329). Required for maintenance of corneocytes and keratin filaments in suprabasal keratinocytes in the epidermis of the ear, potentially via moderation of expression and localization of keratins and their partner proteins (By similarity). Plays a role in the establishment of the epidermal barrier on plantar skin (By similarity).

See full target information KRT2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Oncotarget 7:78242-78254 PubMed27793041

2016

CPI-17 drives oncogenic Ras signaling in human melanomas via Ezrin-Radixin-Moesin family proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Lars Björn Riecken,Ansgar Zoch,Ulrike Wiehl,Sabine Reichert,Ingmar Scholl,Yan Cui,Mirjana Ziemer,Ulf Anderegg,Christian Hagel,Helen Morrison

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 2e antibody [EPR10911] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 2e antibody [EPR10911] - BSA and Azide free (AB249533)

Western blot - Anti-Cytokeratin 2e antibody [EPR10911] - BSA and Azide free (AB249533)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Sample Prep & Detection Kits

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise