AB9005

Anti-Cytokeratin 5 + 8 antibody [RCK102]

Name: Anti-Cytokeratin 5 + 8 antibody [RCK102]
Brand: Abcam Limited
SKU: AB9005
Rating: 5 (1 reviews)

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(1 리뷰)

(9 제품이 사용된 논문 )

Mouse Monoclonal Cytokeratin 5 antibody. Suitable for IHC-P, Flow Cyt, IHC-Fr and reacts with Human samples. Cited in 9 publications. Immunogen corresponding to Cell preparation containing MR21 cell line protein.

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Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 + 8 antibody [RCK102] (AB9005)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 + 8 antibody [RCK102] (AB9005)

Immunohistochemistry analysis on frozen sections of human liver hepatocytes and bile ducts labeling Cytokeratin 5 + 8 using ab9005.

Flow Cytometry - Anti-Cytokeratin 5 + 8 antibody [RCK102] (AB9005)

Flow Cyt

Unknown

Flow Cytometry - Anti-Cytokeratin 5 + 8 antibody [RCK102] (AB9005)

Overlay histogram showing A431 cells stained with ab9005 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab9005, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A431 cells fixed with 100% methanol (5 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 + 8 antibody [RCK102] (AB9005)

Immunohistochemistry analysis on frozen sections of human urinary bladder epithelium labeling Cytokeratin 5 + 8 using ab9005.

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 + 8 antibody [RCK102] (AB9005)

Immunohistochemistry analysis on frozen sections of human liver hepatocytes labeling Cytokeratin 5 + 8 using ab9005.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 8 antibody [RCK102] (AB9005)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 8 antibody [RCK102] (AB9005)

IHC image of ab9005 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab9005, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

RCK102

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-Fr, IHC-P, Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

This antibody reacts with cytokeratin 5 and cytokeratin 8, recognizing virtually all epithelial tissues and carcinomas.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100 - 1/200", "FlowCyt-species-notes": "<a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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제품 세부 정보

Cytokeratins are a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. They range in molecular weight between 40 kDa and 68 kDa and isoelectric pH between 4.9 – 7.8. The individual human cytokeratins are designated 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

Preservative: 0.09% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for the formation of keratin intermediate filaments in the basal epidermis and maintenance of the skin barrier in response to mechanical stress (By similarity). Regulates the recruitment of Langerhans cells to the epidermis, potentially by modulation of the abundance of macrophage chemotactic cytokines, macrophage inflammatory cytokines and CTNND1 localization in keratinocytes (By similarity).

See full target information KRT5

Additional targets

KRT8

대체 명칭 보기

58 kDa cytokeratin, Cytokeratin-5, Keratin-5, Type-II keratin Kb5, CK-5, K5, KRT5, CK5, CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

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제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in oncology 11:752331 PubMed34956876

2021

Circadian Influences on Chemotherapy Efficacy in a Mouse Model of Brain Metastases of Breast Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

William H Walker,Samuel A Sprowls,Jacob R Bumgarner,Jennifer A Liu,O Hecmarie Meléndez-Fernández,James C Walton,Paul R Lockman,A Courtney DeVries,Randy J Nelson

Regenerative medicine 14:15-31 PubMed30566028

2018

The effect of mesenchymal stem cell-secreted factors on airway epithelial repair.

Applications

Unspecified application

Species

Unspecified reactive species

Nur Ssa Halim,Wan M Aizat,Badrul H Yahaya

Scientific reports 7:11732 PubMed28916766

2017

Stimulatory Secretions of Airway Epithelial Cells Accelerate Early Repair of Tracheal Epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Egi Kardia,Rafeezul Mohamed,Badrul Hisham Yahaya

Translational psychiatry 2:e139 PubMed22781172

2012

A molecular characterization of the choroid plexus and stress-induced gene regulation.

Applications

IHC-Fr

Species

Rat

M Sathyanesan,M J Girgenti,M Banasr,K Stone,C Bruce,E Guilchicek,K Wilczak-Havill,A Nairn,K Williams,S Sass,J G Duman,S S Newton

Journal of immunology (Baltimore, Md. : 1950) 184:1918-30 PubMed20083673

2010

T cell/transmembrane, Ig, and mucin-3 allelic variants differentially recognize phosphatidylserine and mediate phagocytosis of apoptotic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Rosemarie H DeKruyff,Xia Bu,Angela Ballesteros,César Santiago,Yee-Ling E Chim,Hyun-Hee Lee,Piia Karisola,Muriel Pichavant,Gerardo G Kaplan,Dale T Umetsu,Gordon J Freeman,José M Casasnovas

American journal of physiology. Lung cellular and 293:L69-76 PubMed17384084

2007

Bronchial epithelial cell growth regulation in fibroblast cocultures: the role of hepatocyte growth factor.

Applications

Unspecified application

Species

Unspecified reactive species

Grzegorz Skibinski,J Stuart Elborn,Madeleine Ennis

The American journal of pathology 136:641-55 PubMed1690512

1990

Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas.

Applications

Unspecified application

Species

Unspecified reactive species

F Ramaekers,C van Niekerk,L Poels,E Schaafsma,A Huijsmans,H Robben,G Schaart,P Vooijs

The American journal of pathology 136:657-68 PubMed1690513

1990

Changing patterns of keratin expression during progression of cervical intraepithelial neoplasia.

Applications

Unspecified application

Species

Unspecified reactive species

F Smedts,F Ramaekers,H Robben,M Pruszczynski,G van Muijen,B Lane,I Leigh,P Vooijs

The American journal of pathology 136:329-43 PubMed1689541

1990

Distribution of cytokeratin polypeptides in human transitional cell carcinomas, with special emphasis on changing expression patterns during tumor progression.

Applications

Unspecified application

Species

Unspecified reactive species

H E Schaafsma,F C Ramaekers,G N van Muijen,E B Lane,I M Leigh,H Robben,A Huijsmans,E C Ooms,D J Ruiter

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 5 + 8 antibody [RCK102]

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 + 8 antibody [RCK102] (AB9005)

Flow Cytometry - Anti-Cytokeratin 5 + 8 antibody [RCK102] (AB9005)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 + 8 antibody [RCK102] (AB9005)

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 5 + 8 antibody [RCK102] (AB9005)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 + 8 antibody [RCK102] (AB9005)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

Additional targets

대체 명칭 보기

제품이 사용된 논문 (9)

Product promise