Anti-Cytokeratin 6 antibody [EPR1603Y]

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 6 antibody [EPR1603Y] (AB52620)

ab52620 at 1/100-1/250 dilution staining A431 cells.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 6 antibody [EPR1603Y] (AB52620)

Immunocytochemistry/Immunofluorescence analysis of HaCaT (Human keratinocyte cell line) labelling Cytokeratin 6 with purified ab52620 at 1/1000. Cells were fixed with 4% PFA and permeabilized with 0.1% triton X-100. ab150077 Goat anti rabbit IgG (Alexa Fluor^® 488) at 1/1000 was used as the secondary antibody. Nuclei were counterstained with DAPI. PBS was used instead of the primary antibody as the negative control.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 6 antibody [EPR1603Y] (AB52620)

ab52620 at 1/100-1/250 dilution staining human squamous cervical carcinoma; paraffin embedded.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 6 antibody [EPR1603Y] (AB52620)

IHC image of Cytokeratin 6 staining in a section of formalin-fixed paraffin-embedded normal human skin* performed on a Leica Biosystems BOND® RX instrument. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab52620, 1ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody. For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

• WB

Unknown

Western blot - Anti-Cytokeratin 6 antibody [EPR1603Y] (AB52620)

All lanes:

Western blot - Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620) at 1/100000 dilution

All lanes:

A431 cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP antibody at 1/2000 dilution

Predicted band size: 60 kDa

Observed band size: 60 kDa

false

• WB

Lab

Western blot - Anti-Cytokeratin 6 antibody [EPR1603Y] (AB52620)

Blocking buffer : 5% NFDM /TBST.

All lanes:

Western blot - Anti-Cytokeratin 6 antibody [EPR1603Y] (ab52620) at 1/1000 dilution

Lane 1:

N-GST tagged full-length recombinant human Cytokeratin 6A protein at 10 ng

Lane 2:

N-GST tagged full-length recombinant human Cytokeratin 5 protein at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 87 kDa

false

Exposure time: 180s