AB199718

Anti-Cytokeratin 7 antibody [EPR17079]

Name: Anti-Cytokeratin 7 antibody [EPR17079]
Brand: Abcam Limited
SKU: AB199718
Rating: 5 (1 reviews)

RabMAb
Recombinant
각 태그의 뜻

(1 리뷰)

(6 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Cytokeratin 7 antibody. Suitable for IP, WB, IHC-P and reacts with Mouse, Rat samples. Cited in 6 publications.

대체 명칭 보기

Krt2-7, Krt7, Cytokeratin-7, Keratin-7, Type-II keratin Kb7, CK-7, K7, CK7

6 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse colon tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Cytokeratin 7 with ab199718 at 1/1600 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on mouse lung tissue is observed. Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Supplier Data

Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718)

Cytokeratin 7 was immunoprecipitated from 1mg of Mouse skin whole cell extract with ab199718 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199718 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : Mouse skin whole cell extract 10 μg (Input). Lane 2 : ab199718 IP in Mouse skin whole cell extract. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199718 in Mouse skin whole cell extract.
Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718)

Predicted band size: 51 kDa

Observed band size: 45 kDa

false

Supplier Data

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718)

~45 kDa bands are supported by the literature and competitor's products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID : 16265353.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/20000 dilution

All lanes:

Mouse skin tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

Observed band size: 45 kDa,51 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718)

~45 kDa bands are supported by the literature and competitor's products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID : 16265353.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/4000 dilution

Lane 1:

Rat bladder tissue lysate at 10 µg

Lane 2:

Rat lung tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa

Observed band size: 45 kDa,51 kDa

false

Exposure time: 15s

Supplier Data

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718)

~45 kDa bands are supported by the literature and competitor's products, which may be an isoform. The observed MW is consistent with what has been described in the literature PMID : 16265353.

Blocking/dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (ab199718) at 1/20000 dilution

Lane 1:

Mouse lung tissue lysate at 10 µg

Lane 2:

Mouse bladder tissue lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 51 kDa,55 kDa

Observed band size: 45 kDa,51 kDa,55 kDa

false

Exposure time: 15s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17079

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat

Applications

IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/800", "IHCP-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/4000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Blocks interferon-dependent interphase and stimulates DNA synthesis in cells.

See full target information Krt7

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Biomedical reports 20:61 PubMed38476609

2024

Metastatic gallbladder cancer presenting as numb chin syndrome: A case report and literature review.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Murahashi,Edward Hosea Ntege,Kentaro Ide,Nobuyuki Maruyama,Jumpei Shirakawa,Hiroki Koyama,Toshihiro Kawano,Takahiro Goto,Yusuke Shimizu,Kazuhide Nishihara,Hiroyuki Nakamura

Molecular and clinical oncology 19:83 PubMed37745262

2023

Metastatic colon carcinoma in the maxilla: Highlighting the importance of perioperative oral management: A case report.

Applications

Unspecified application

Species

Unspecified reactive species

Murahashi Makoto,Edward Hosea Ntege,Nishihara Kazuhide,Ide Kentaro,Shirakawa Jumpei,Maruyama Nobuyuki,Kawano Toshihiro,Shimizu Yusuke,Nakamura Hiroyuki

Cells 12: PubMed37048098

2023

Autophagy Inhibitor Chloroquine Downmodulates Hepatic Stellate Cell Activation and Liver Damage in Bile-Duct-Ligated Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Trinh Van Le,Hong-Thuy Phan-Thi,My-Xuan Huynh-Thi,Thanh Minh Dang,Ai Xuan Le Holterman,Gabriele Grassi,Thao-Uyen Nguyen-Luu,Nhung Hai Truong

Molecular medicine (Cambridge, Mass.) 28:119 PubMed36153499

2022

LINC00240/miR-155 axis regulates function of trophoblasts and M2 macrophage polarization via modulating oxidative stress-induced pyroptosis in preeclampsia.

Applications

Unspecified application

Species

Unspecified reactive species

Hai-Ying Wu,Kan Liu,Jing-Li Zhang

Journal of nanobiotechnology 20:79 PubMed35164767

2022

Extracellular microparticles derived from hepatic progenitor cells deliver a death signal to hepatoma-initiating cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaojuan Hou,Wenting Liu,Xue Yang,Changchun Shao,Lu Gao,Li Zhang,Lixin Wei

Frontiers in endocrinology 9:616 PubMed30386299

2018

The Effect of Hypoxia and Metformin on Fatty Acid Uptake, Storage, and Oxidation in L6 Differentiated Myotubes.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Musutova,Moustafa Elkalaf,Natalie Klubickova,Michal Koc,Stanislav Povysil,Jan Rambousek,Beatriz Volckaert,Frantisek Duska,Minh Duc Trinh,Martin Kalous,Jan Trnka,Kamila Balusikova,Jan Kovar,Jan Polak

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 7 antibody [EPR17079]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718)

Immunoprecipitation - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718)

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718)

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718)

Western blot - Anti-Cytokeratin 7 antibody [EPR17079] (AB199718)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (6)

Product promise