Anti-Cytokeratin 8 antibody

• IHC-P

AbReview56665****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (AB59400)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Cytokeratin 8 with ab59400 at 1/100 dilution. Tissue sections were fixed with formaldehyde; heat mediated antigen retrieval was performed using a citric acid. 2% BSA was used to block, followed by incubation with ab59400 in TBS/BSA/azide for 2 hours at 21°C. a polyclonal goat anti-rabbit IgG bitin conjugated secondary antibody was used at 1/300 dilution.

This image is courtesy of an abreview submitted by Carl Hobbs, King's College London, United Kingdom

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (AB59400)

Immunocytochemistry/ Immunofluorescence analysis of HeLa cells, labeling Cytokeratin 8 with ab59400. The picture on the right is blocked with the synthesized peptide.

• IHC-P

AbReview23440****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (AB59400)

ab59400 staining Cytokeratin 8 in Human breast cancer cells xenografted into nude mice by Immunohistochemistry (Formalin/ PFA-fixed paraffin-embedded tissue sections). The sections were fixed in formalin and subjected to heat-mediated antigen retrieval in citrate buffer (0.1M Sodium Citrate) prior to blocking with 5% serum for 1 hour at 4°C. The primary antibody was diluted 1/200 in 5% goat serum in PBS and incubated with the sample for 12 hours at 4°C. A Biotin-conjugated Goat anti-Rabbit polyclonal was used as the secondary antibody, diluted 1/1000.

This image is courtesy of an Abreview provided by Megha Rajaram.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (AB59400)

Immunohistochemical analysis of paraffin embedded human colon carcinoma tissue using ab59400 at 1/50 dilution, in the presence (right) and absence (left) of immunising peptide. Then, a polymer secondary antibody was used for detection.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody (AB59400)

ICC/IF image of ab59400 stained MCF7 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab59400, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• WB

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Western blot - Anti-Cytokeratin 8 antibody (AB59400)

All lanes:

Western blot - Anti-Cytokeratin 8 antibody (ab59400) at 1/500 dilution

Lane 1:

EGF treated (200ng/ml, 30mins) 293 cell extracts

Lane 2:

EGF treated (200ng/ml, 30mins) 293 cell extracts with immunising peptide

Predicted band size: 53 kDa

Observed band size: 54 kDa

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