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AB227644

Anti-Cytokeratin 8 antibody [SP102] - C-terminal

4

(1 리뷰)

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(2 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Cytokeratin 8 antibody. C-terminal. Suitable for IHC-P, Flow Cyt (Intra), ICC/IF, IHC-Fr and reacts with Human, Mouse, Rat samples. Cited in 2 publications.

대체 명칭 보기

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

17 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human lung tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human stomach tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human bladder tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human breast tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Immunocytochemistry/ Immunofluorescence analysis of HaCaT (human skin keratinocyte) cells labeling Cytokeratin 8 with purified ab227644 at 1 : 25 (5.48 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1 : 200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1 : 1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human lung squamous cell carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human bladder transitional cell carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human prostate adenocarcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human stomach adenocarcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Flow Cytometry analysis of HaCaT (human skin keratinocyte) cells labeling Cytokeratin 8 with purified ab227644 at 1 : 200 dilution (0.685 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human placenta tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human prostate tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human breast ductal carcinoma tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Formalin-fixed, paraffin-embedded human thyroid tissue stained for Cytokeratin 8 using ab227644 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse small intestine tissue labeling Cytokeratin with ab227644 at 1/20 (6.85 μg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 μg/ml) (Green). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab227644 for 60 mins at room temperature. Confocal image showing positive staining on mouse small intestine tissue. The nuclear counterstain was DAPI (Blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody only control : Primary diluent was used instead of primary antibody, followed by secondary antibody ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Cytokeratin 8 antibody [SP102] - C-terminal (AB227644)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat small intestine tissue labeling Cytokeratin with ab227644 at 1/20 (6.85 μg/ml) dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (2 μg/ml) (Green). The immunostaining was performed on a Leica Biosystems BOND® RX instrument. The section was incubated with ab227644 for 60 mins at room temperature. Confocal image showing positive staining on rat small intestine tissue. The nuclear counterstain was DAPI (Blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody only control : Primary diluent was used instead of primary antibody, followed by secondary antibody ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

관련 conjugated 항체와 다양한 조성의 항체 (2)

  • Carrier free

    Anti-Cytokeratin 8 antibody [SP102] - BSA and Azide free

  • Biotin

    Biotin Anti-Cytokeratin 8 antibody [SP102] - C-terminal

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP102

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-Fr, ICC/IF, IHC-P, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB154301

Anti-Cytokeratin 8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody (AB154301)

  • 4
  • 1
Primary Antibodies

AB210139

Alexa Fluor® 405 Anti-Cytokeratin 8 antibody [EP1628Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 405 Anti-Cytokeratin 8 antibody [EP1628Y] (AB210139)

  • 4
  • 1
Primary Antibodies

AB209297

PE Anti-Cytokeratin 8 antibody [EP1628Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-Cytokeratin 8 antibody [EP1628Y] (AB209297)

  • 0
  • 0
Primary Antibodies

AB192467

Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Alexa Fluor® 488 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192467)

  • 5
  • 5
Primary Antibodies

AB192468

Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Cytokeratin 8 antibody [EP1628Y] (AB192468)

  • 5
  • 1
Primary Antibodies

AB217173

Anti-Cytokeratin 8 antibody [EP1628Y] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Flow Cytometry (Intracellular) - Anti-Cytokeratin 8 antibody [EP1628Y] - BSA and Azide free (AB217173)

  • 5
  • 2
Primary Antibodies

AB53280

Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker

KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 antibody [EP1628Y] - Cytoskeleton Marker (AB53280)

  • 5
  • 9

보완 제품

Assay Kits

AB267810

Human Cytokeratin 8 ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Cytokeratin 8 ELISA Kit (AB267810)

  • 0
  • 0
Primary Antibodies

AB52635

Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 5 antibody [EP1601Y] - Cytoskeleton Marker (AB52635)

  • 5
  • 11
Primary Antibodies

AB52625

Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Multiplex immunohistochemistry - Anti-Cytokeratin 19 antibody [EP1580Y] - Cytoskeleton Marker (AB52625)

  • 5
  • 12
Primary Antibodies

AB185628

Anti-Cytokeratin 1 antibody [EPR17744]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 1 antibody [EPR17744] (AB185628)

  • 0
  • 0
Primary Antibodies

AB64088

Anti-CD20 antibody [SP32]

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD20 antibody [SP32] (AB64088)

  • 5
  • 12
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>Primary antibody incubation for 30 minutes at room temperature.</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/20", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/20", "IHCFr-species-notes": "<p></p>" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A/G
Purification 관련 사항
Purified from TCS by protein A/G.
보관 버퍼
pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
See full target information KRT8
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Environmental toxicology 38:2867-2880 PubMed37565747

2023

Assessing the double-edged of extracellular signal-regulated kinase/CCAAT-enhancer-binding protein beta signaling pathway in arsenic-induced skin damage and its potential foodborne interventions.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Yang,Dexiu Hu,Sufei Du,Liping Wu,Maoyuan Gong,Yuhong Zhang,Xingcan Yang,Yang Yang,Ruobi Chen,Yuyan Xu,Qibing Zeng

Cancer research 82:648-664 PubMed34853070

2021

Multiomics Analysis of Spatially Distinct Stromal Cells Reveals Tumor-Induced O-Glycosylation of the CDK4-pRB Axis in Fibroblasts at the Invasive Tumor Edge.

Applications

Unspecified application

Species

Unspecified reactive species

Gina Bouchard,Fernando Jose Garcia-Marques,Loukia Georgiou Karacosta,Weiruo Zhang,Abel Bermudez,Nicholas McIlvain Riley,Sushama Varma,Lindsey Catherine Mehl,Jalen Anthony Benson,Joseph B Shrager,Carolyn Ruth Bertozzi,Sharon J Pitteri,Amato J Giaccia,Sylvia Katina Plevritis
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

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