AB76584

Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y]

BOND RX™ Validated
RabMAb
Recombinant
각 태그의 뜻

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(3 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Cytokeratin 8 phospho S23 antibody. Suitable for IHC-P, WB, Dot and reacts with Human, Synthetic peptide samples. Cited in 3 publications.

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (AB76584)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (AB76584)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling Cytokeratin 8 with ab76584 at 1/3600 dilution (0.035 µg/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). The section was incubated with ab76584 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 epitope retrieval solution 2) for 20 mins.

Supplier Data

Western blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (AB76584)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584) at 1/10000 dilution

Lane 1:

Untreated HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate at 15 µg

Lane 2:

HeLa treated with 100nM Calyculin A for 30mins whole cell lysate at 15 µg

Lane 3:

HeLa treated with 100nM Calyculin A for 30mins whole cell lysate, then the membrane treated with Alkaline Phosphatase for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 54 kDa

Observed band size: 54 kDa

false

Exposure time: 7s

Supplier Data

Dot Blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (AB76584)

Dot blot analysis of Cytokeratin 8 (phospho S23) using ab76584 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1/100,000 dilution. Blocking and dilution buffer : 5% NFDM/TBST. Exposure time : 180s.

All lanes:

Dot Blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (ab76584) at 1/1000 dilution

Lane 1:

Cytokeratin 8 (phospho S23) peptide

Lane 2:

EGFR non-phospho peptide

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 180s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1629Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Dot, WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/3600", "IHCP-species-notes": " Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000", "WB-species-notes": "", "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "" }, "Synthetic peptide": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "1/1000", "Dot-species-notes": "" } } }

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제품 세부 정보

This product has switched from a hybridoma to recombinant production method on 23rd February 2024.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Required for the formation of KRT8/KRT18 filaments that are involved in ARHGEF40-mediated actin stress fiber formation and tensional force-induced stress fiber formation and reinforcement (PubMed : 26823019). Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

See full target information KRT8 pS23

대체 명칭 보기

CYK8, KRT8, Cytokeratin-8, Keratin-8, Type-II keratin Kb8, CK-8, K8

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Developmental cell 59:2347-2363.e9 PubMed38843837

2024

An integrated approach identifies the molecular underpinnings of murine anterior visceral endoderm migration.

Applications

Unspecified application

Species

Unspecified reactive species

Shifaan Thowfeequ,Jonathan Fiorentino,Di Hu,Maria Solovey,Sharon Ruane,Maria Whitehead,Felix Zhou,Jonathan Godwin,Yentel Mateo-Otero,Bart Vanhaesebroeck,Antonio Scialdone,Shankar Srinivas

Scientific reports 6:26557 PubMed27222304

2016

FAM83H and casein kinase I regulate the organization of the keratin cytoskeleton and formation of desmosomes.

Applications

Unspecified application

Species

Unspecified reactive species

Takahisa Kuga,Mitsuho Sasaki,Toshinari Mikami,Yasuo Miake,Jun Adachi,Maiko Shimizu,Youhei Saito,Minako Koura,Yasunori Takeda,Junichiro Matsuda,Takeshi Tomonaga,Yuji Nakayama

Toxicology and applied pharmacology 238:71-9 PubMed19409407

2009

Cytokeratin 8/18 overexpression and complex formation as an indicator of GST-P positive foci transformation into hepatocellular carcinomas.

Applications

IHC-Fr

Species

Unspecified reactive species

Anna Kakehashi,Masayo Inoue,Min Wei,Shoji Fukushima,Hideki Wanibuchi

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (AB76584)

Western blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (AB76584)

Dot Blot - Anti-Cytokeratin 8 (phospho S23) antibody [EP1629Y] (AB76584)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise