AB51601

Anti-DAPK2 antibody [EP1633Y]

Name: Anti-DAPK2 antibody [EP1633Y]
Brand: Abcam Limited
SKU: AB51601
Rating: 4 (1 reviews)

20ul selling size
RabMAb
각 태그의 뜻

(1 리뷰)

(4 제품이 사용된 논문 )

Rabbit Monoclonal DAPK2 antibody. Suitable for IHC-P, WB, Flow Cyt (Intra) and reacts with Human, Rat samples. Cited in 4 publications.

2 이미지

Flow Cytometry (Intracellular) - Anti-DAPK2 antibody [EP1633Y] (AB51601)

Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-DAPK2 antibody [EP1633Y] (AB51601)

Overlay histogram showing HeLa cells stained with ab51601 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51601, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluorr^® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x10⁶ cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

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Western blot - Anti-DAPK2 antibody [EP1633Y] (AB51601)

All lanes:

Western blot - Anti-DAPK2 antibody [EP1633Y] (ab51601) at 1/2000 dilution

All lanes:

Rat spleen lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 43 kDa

Observed band size: 42 kDa

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1633Y

Isotype

IgG

Carrier free

Reacts with

Rat, Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/1000", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

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특성 및 보관 정보

제형

Liquid

Purity

Tissue culture supernatant

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Calcium/calmodulin-dependent serine/threonine kinase involved in multiple cellular signaling pathways that trigger cell survival, apoptosis, and autophagy. Regulates both type I apoptotic and type II autophagic cell death signals, depending on the cellular setting. The former is caspase-dependent, while the latter is caspase-independent and is characterized by the accumulation of autophagic vesicles. Acts as a mediator of anoikis and a suppressor of beta-catenin-dependent anchorage-independent growth of malignant epithelial cells. May play a role in granulocytic maturation (PubMed : 17347302). Regulates granulocytic motility by controlling cell spreading and polarization (PubMed : 24163421).. Isoform 2 is not regulated by calmodulin. It can phosphorylate MYL9. It can induce membrane blebbing and autophagic cell death.

See full target information Death-associated protein kinase 2

대체 명칭 보기

Death-associated protein kinase 2, DAP kinase 2, DAP-kinase-related protein 1, DRP-1, DAPK2

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 9:1759 PubMed29717115

2018

Non-canonical activation of DAPK2 by AMPK constitutes a new pathway linking metabolic stress to autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Ruth Shiloh,Yuval Gilad,Yaara Ber,Miriam Eisenstein,Dina Aweida,Shani Bialik,Shenhav Cohen,Adi Kimchi

Autophagy 12:2271-2285 PubMed27653365

2016

Thyroid hormone suppresses hepatocarcinogenesis via DAPK2 and SQSTM1-dependent selective autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Hsiang-Cheng Chi,Shen-Liang Chen,Chung-Ying Tsai,Wen-Yu Chuang,Ya-Hui Huang,Ming-Ming Tsai,Sheng-Ming Wu,Cheng-Pu Sun,Chau-Ting Yeh,Kwang-Huei Lin

Cell death and differentiation 22:465-75 PubMed25361081

2014

DAPK2 is a novel regulator of mTORC1 activity and autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Y Ber,R Shiloh,Y Gilad,N Degani,S Bialik,A Kimchi

PloS one 6:e17344 PubMed21408167

2011

New modularity of DAP-kinases: alternative splicing of the DRP-1 gene produces a ZIPk-like isoform.

Applications

Species

Human

Yishay Shoval,Hanna Berissi,Adi Kimchi,Shmuel Pietrokovski

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DAPK2 antibody [EP1633Y]

Flow Cytometry (Intracellular) - Anti-DAPK2 antibody [EP1633Y] (AB51601)

Western blot - Anti-DAPK2 antibody [EP1633Y] (AB51601)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purity

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (4)

Product promise