AB325143

Anti-DARC antibody [EPR29719-512] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal DARC antibody. Carrier free. Suitable for Flow Cyt, IHC-P, WB and reacts with Mouse samples.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

This data was developed using ab325127, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse bone marrow tissue labeling DARC with ab325127 at 1/500 (0.988 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on erythrocytes and endothelium in mouse bone marrow (PMID : 28553950). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

This data was developed using ab325127, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cardiac muscle tissue labeling DARC with ab325127 at 1/500 (0.988 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression : weak staining in mouse cardiac muscle. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

This data was developed using ab325127, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling DARC with ab325127 at 1/500 (0.988 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on endothelium in mouse liver. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

This data was developed using ab325127, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling DARC with ab325127 at 1/500 (0.988 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on erythrocytes in mouse spleen (PMID : 28553950). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Flow Cytometry - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

This data was developed using ab325127, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse PBMC (mouse primary peripheral blood mononuclear cell) cells labelling DARC with ab325127 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.

Flow Cyt

Supplier Data

Flow Cytometry - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

This data was developed using ab325127, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse blood cells labelling DARC with ab325127 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells were co-stained with anti mouse TER-119 conjugated to Alexa Fluor®647.

Supplier Data

Western blot - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

This data was developed using ab325127, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : testis, skeletal muscle, heart.

High expression : bone marrow (PMID : 37006247, 26908826, 28553950), spleen (PMID : 7699323).

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 37153544).

The identity of the higher than 250 kDa (in lane 4) is unknown.

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

All lanes:

Western blot - Anti-DARC antibody [EPR29719-512] (<a href='/ko/products/primary-antibodies/darc-antibody-epr29719-512-ab325127'>ab325127</a>) at 1/1000 dilution

Lane 1:

Mouse bone marrow tissue at 20 µg

Lane 2:

Mouse spleen tissue lysate at 20 µg

Lane 3:

Mouse testis tissue at 20 µg

Lane 4:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 5:

Mouse heart tissue lysate at 20 µg

Secondary

All lanes:

Western blot at 1/20000 dilution

Observed band size: 37-50 kDa,124 kDa

false

Exposure time: 37s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29719-512

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

IHC-P, Flow Cyt, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

ab325143 is the carrier-free version of ab325127

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Atypical chemokine receptor that controls chemokine levels and localization via high-affinity chemokine binding that is uncoupled from classic ligand-driven signal transduction cascades, resulting instead in chemokine sequestration, degradation, or transcytosis. Also known as interceptor (internalizing receptor) or chemokine-scavenging receptor or chemokine decoy receptor. Has a promiscuous chemokine-binding profile, interacting with inflammatory chemokines of both the CXC and the CC subfamilies but not with homeostatic chemokines. Acts as a receptor for chemokines including CCL2, CCL5, CCL7, CCL11, CCL13, CCL14, CCL17, CXCL5, CXCL6, IL8/CXCL8, CXCL11, GRO, RANTES, MCP-1 and TARC. May regulate chemokine bioavailability and, consequently, leukocyte recruitment through two distinct mechanisms : when expressed in endothelial cells, it sustains the abluminal to luminal transcytosis of tissue-derived chemokines and their subsequent presentation to circulating leukocytes; when expressed in erythrocytes, serves as blood reservoir of cognate chemokines but also as a chemokine sink, buffering potential surges in plasma chemokine levels (By similarity).. (Microbial infection) Acts as a receptor for the malaria parasite Plasmodium yoelii in mature erythrocytes but not reticulocytes.

See full target information Ackr1

대체 명칭 보기

CD234, Darc, Dfy, Fy, Atypical chemokine receptor 1, Duffy antigen/chemokine receptor

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DARC antibody [EPR29719-512] - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

Flow Cytometry - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

Flow Cytometry - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

Western blot - Anti-DARC antibody [EPR29719-512] - BSA and Azide free (AB325143)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise