Anti-DAZAP1 antibody

• IP

Supplier Data

Immunoprecipitation - Anti-DAZAP1 antibody (AB240604)

DAZAP1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) using ab240604. For western blotting, ab240604 was used at 1 μg/ml.

Lane 1 : ab240604 used for IP at 6 μg/mg lysate.
Lane 2 : Control IgG.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-DAZAP1 antibody (ab240604)

Predicted band size: 43 kDa

true

Exposure time: 30s

• WB

Supplier Data

Western blot - Anti-DAZAP1 antibody (AB240604)

All lanes:

Western blot - Anti-DAZAP1 antibody (ab240604) at 0.4 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 50 µg

Lane 4:

TCMK-1 (mouse kidney epithelial cell line) cell lysate at 50 µg

Lane 5:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate at 50 µg

Predicted band size: 43 kDa

true

Exposure time: 10s