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AB250616

Anti-DAZAP1 antibody [EPR14400] - BSA and Azide free

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(1 출판물)

Rabbit Recombinant Monoclonal DAZAP1 antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

DAZ-associated protein 1, Deleted in azoospermia-associated protein 1, DAZAP1

3 이미지
Flow Cytometry (Intracellular) - Anti-DAZAP1 antibody [EPR14400] - BSA and Azide free (AB250616)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DAZAP1 antibody [EPR14400] - BSA and Azide free (AB250616)

This data was developed using ab182558, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed Jurkat cells labeling DAZAP1 with ab182558 at 1/10 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Immunoprecipitation - Anti-DAZAP1 antibody [EPR14400] - BSA and Azide free (AB250616)
  • IP

Supplier Data

Immunoprecipitation - Anti-DAZAP1 antibody [EPR14400] - BSA and Azide free (AB250616)

This data was developed using ab182558, the same antibody clone in a different buffer formulation.

Western blot analysis on immunoprecipitation pellet from Jurkat cell lysate immunoprecipitated using ab182558 at 1/50 dilution (Lane 1). Lane 2 : Negative control.

Secondary : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-DAZAP1 antibody [EPR14400] (<a href='/ko/products/primary-antibodies/dazap1-antibody-epr14400-ab182558'>ab182558</a>)

Predicted band size: 43 kDa

false

Western blot - Anti-DAZAP1 antibody [EPR14400] - BSA and Azide free (AB250616)
  • WB

Supplier Data

Western blot - Anti-DAZAP1 antibody [EPR14400] - BSA and Azide free (AB250616)

This data was developed using ab182558, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DAZAP1 antibody [EPR14400] (<a href='/ko/products/primary-antibodies/dazap1-antibody-epr14400-ab182558'>ab182558</a>) at 1/50000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 43 kDa

Observed band size: 43 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14400

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, IP, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB210655

Alexa Fluor® 594 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 594 Anti-Integrin alpha 2 antibody [EPR17338] - C-terminal (AB210655)

  • 0
  • 0
Primary Antibodies

AB89713

Anti-FGF11 antibody [MM0282-6J20]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FGF11 antibody [MM0282-6J20] (AB89713)

  • 0
  • 0
Assay Kits

AB253778

Human GAPDH Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human GAPDH Antibody Pair - BSA and Azide free (AB253778)

  • 0
  • 0
Primary Antibodies

AB314553

Anti-NCOA4 antibody [EPR28511-12]

20ul selling size|RabMAb|Recombinant

Immunoprecipitation - Anti-NCOA4 antibody [EPR28511-12] (AB314553)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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제품 세부 정보

ab250616 is the carrier-free version of ab182558.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

RNA-binding protein, which may be required during spermatogenesis.
See full target information DAZAP1
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Diagnostic pathology 16:93 PubMed34689819

2021

CircRNA NRIP1 promotes papillary thyroid carcinoma progression by sponging mir-195-5p and modulating the P38 MAPK and JAK/STAT pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Chuang Li,Lijuan Zhu,Lijun Fu,Mingli Han,Ya Li,Zhaozhong Meng,Xinguang Qiu
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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