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AB238151

Anti-DCSTAMP antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal DCSTAMP antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DCSTAMP aa 100-250.

대체 명칭 보기

TM7SF4, DCSTAMP, Dendritic cell-specific transmembrane protein, DC-STAMP, hDC-STAMP, Dendrocyte-expressed seven transmembrane protein, IL-four-induced protein, Transmembrane 7 superfamily member 4, FIND

3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCSTAMP antibody (AB238151)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCSTAMP antibody (AB238151)

Paraffin-embedded human liver tissue stained for DCSTAMP using ab238151 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunocytochemistry/ Immunofluorescence - Anti-DCSTAMP antibody (AB238151)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DCSTAMP antibody (AB238151)

HepG2 (human liver hepatocellular carcinoma cell line) cells stained for DCSTAMP (green) using ab238151 at 1/66 dilution in ICC/IF. Secondary antibody is Alexa Fluor® 488-conjugated Goat Anti-Rabbit IgG (H+L). The nuclear counter stain is DAPI.

The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCSTAMP antibody (AB238151)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCSTAMP antibody (AB238151)

Paraffin-embedded human kidney tissue stained for DCSTAMP using ab238151 at 1/200 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human DCSTAMP aa 100-250. The exact immunogen used to generate this antibody is proprietary information.

Q9H295

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

Probable cell surface receptor that plays several roles in cellular fusion, cell differentiation, bone and immune homeostasis. Plays a role in TNFSF11-mediated osteoclastogenesis. Cooperates with OCSTAMP in modulating cell-cell fusion in both osteoclasts and foreign body giant cells (FBGCs). Participates in osteoclast bone resorption. Involved in inducing the expression of tartrate-resistant acid phosphatase in osteoclast precursors. Plays a role in haematopoietic stem cell differentiation of bone marrow cells toward the myeloid lineage. Inhibits the development of neutrophilic granulocytes. Plays also a role in the regulation of dendritic cell (DC) antigen presentation activity by controlling phagocytic activity. Involved in the maintenance of immune self-tolerance and avoidance of autoimmune reactions.
See full target information DCSTAMP

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 15:33686 PubMed41023001

2025

The role and mechanism of transcription factor KLF4 in regulating mitochondrial damage and apoptosis by activating chondrocyte autophagy in osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Wenguang Feng,Haibin Wang,Guoliang Zhang,Wang Gao,Hongcang Li,Haibo Liu,Yanhong Sun

Journal of experimental orthopaedics 10:68 PubMed37400744

2023

Development and characterization of an intra-articular fracture mediated model of post-traumatic osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Michael S Valerio,William A Pace,Connor P Dolan,Jorge B Edwards,Naveena B Janakiram,Benjamin K Potter,Christopher L Dearth,Stephen M Goldman

Bioengineered 13:2285-2295 PubMed35034537

2022

Interferon-induced protein with tetratricopeptide repeats 1 (IFIT1) accelerates osteoclast formation by regulating signal transducer and activator of transcription 3 (STAT3) signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanliang Xue,Chuanliang Zhao,Tao Liu
제품이 사용된 논문 모두 보기

Product promise

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