AB96004

Anti-DCTN1/p150-glued antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal DCTN1/p150-glued antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human DCTN1 aa 1200 to C-terminus.

5 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (AB96004)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (AB96004)

Immunofluorescence analysis of methanol-fixed HeLa, using ab96004 antibody (Green) at 1 : 500 dilution. Alpha-tubulin filaments were labeled (Red) at 1 : 2000.

Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody (AB96004)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody (AB96004)

Immunofluorescence analysis of methanol-fixed HeLa cells, using ab96004 at 1/200 dilution. Lower image costained with Hoerchst 33342.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (AB96004)

Immunohistochemical analysis of paraffin-embedded Rat brain, using ab96004 at 1/500 dilution. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Unknown

Western blot - Anti-DCTN1/p150-glued antibody (AB96004)

5% SDS PAGE

All lanes:

Western blot - Anti-DCTN1/p150-glued antibody (ab96004) at 1/3000 dilution

All lanes:

Jurkat whole cell lysate at 30 µg

Predicted band size: 141 kDa

false

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (AB96004)

Immunohistochemical analysis of paraffin-embedded ovarian cancer, using ab96004 at 1/100 dilution. Antigen Retrieval : Trilogy™ (EDTA based, pH 8.0) buffer, 15min

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human DCTN1 aa 1200 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14203

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/200", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 20% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity). Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule (PubMed : 25185702). Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon (PubMed : 23874158). Plays a role in metaphase spindle orientation (PubMed : 22327364). Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitment to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole (PubMed : 23386061). Plays a role in primary cilia formation (PubMed : 25774020).

See full target information DCTN1

대체 명칭 보기

Dynactin subunit 1, 150 kDa dynein-associated polypeptide, DAP-150, p135, p150-glued, DP-150, DCTN1

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 16:328 PubMed40263294

2025

Opposing roles for GSK3β and ERK1-dependent phosphorylation of huntingtin during neuronal dysfunction and cell death in Huntington's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas J Krzystek,Rasika Rathnayake,Jia Zeng,Jing Huang,Gary Iacobucci,Michael C Yu,Shermali Gunawardena

Autophagy 19:1199-1220 PubMed36048753

2022

HTT (huntingtin) and RAB7 co-migrate retrogradely on a signaling LAMP1-containing late endosome during axonal injury.

Applications

Unspecified application

Species

Unspecified reactive species

Thomas J Krzystek,Joseph A White,Rasika Rathnayake,Layne Thurston,Hayley Hoffmar-Glennon,Yichen Li,Shermali Gunawardena

Evidence-based complementary and alternative medicine : eCAM 2022:6400517 PubMed35310029

2022

Modified Yuejuwan Inhibited Cholesterol Accumulation and Inflammation in THP-1 Macrophage-Derived Foam Cells by Inhibiting the Activity of the TRIM37/TRAF2/NF-B Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Mingtai Gui,Lei Yao,Bo Lu,Jianhua Li,Jing Wang,Xunjie Zhou,Deyu Fu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DCTN1/p150-glued antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (AB96004)

Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody (AB96004)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (AB96004)

Western blot - Anti-DCTN1/p150-glued antibody (AB96004)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (AB96004)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise