AB302629

Anti-DCTN1/p150-glued antibody [EPR26464-28]

KD Validated
BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal DCTN1/p150-glued antibody. Suitable for IP, Flow Cyt (Intra), ICC/IF, IHC-Fr, IHC-P, WB and reacts with Mouse, Human samples.

11 이미지

Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa (human cervix adenocarcinoma epithelial cell) labelling DCTN1/p150-glued with ab302629 at 1/200 (9.955 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasm staining in HeLa cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin antibody [DM1A] - Loading Control was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab302629 at 1/50 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 594) antibody at 1/1000 dilution.
-ve control 2 : ab7291 at 1/1000 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 dilution.

Flow Cytometry (Intracellular) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (human cervix adenocarcinoma epithelial cell) cells labelling DCTN1/p150-glued with ab302629 at 1/2000 (Red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (Black) . Unlabelled control (cells without incubation with primary antibody and secondary antibody) / Blue.

Lab

Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

DCTN1/p150-glued was immunoprecipitated from 0.35 mg HeLa whole cell lysate with ab302629 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302629 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 10 µg

Lane 2:

ab302629 at 1/30 IP in HeLa whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab302629 in HeLa whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 150 kDa

false

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling DCTN1/p150-glued with ab302629 at 1/200 (9.955 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed antibody at 1/1000 (2ug/ml) dilution (Green).

Confocal image showing cytoplasm staining in Neuro-2a cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin antibody [DM1A] - Loading Control was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Immunohistochemistry (Frozen sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse liver (fresh) tissue labelling DCTN1/p150-glued with ab302629 at 1/100 (19.91 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution (Green).

Negative control : no staining on mouse liver (PMID : 1836789).

The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbedat 1/1000 2 ug/mL dilution.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized Neuro-2a (mouse neuroblastoma neuroblast) cells labelling DCTN1/p150-glued with ab302629 at 1/50 dilution (1ug) (Red) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Immunohistochemical analysis of paraffin-embedded Mouse dorsal root ganglion tissue labelling DCTN1/p150-glued with ab302629 at 1/3500 dilution.

Positive staining on mouse dorsal root ganglion.

The section was incubated with ab302629 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.

IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spinal cord (fresh) tissue labelling DCTN1/p150-glued with ab302629 at 1/100 (19.91 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green).

Confocal image showing positive staining on mouse spinal cord. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/mL dilution.

IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum labelling DCTN1/p150-glued with ab302629 at 1/3500 dilution.

Positive staining on mouse cerebrum.

The section was incubated with ab302629 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 mins.

Lab

Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

DCTN1/p150-glued was immunoprecipitated from 0.35 mg mouse Neuro-2a whole cell lysate with ab302629 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab302629 at 1/1000 dilution.

Blocking and dilution buffer and concentration : 5% NFDM/TBST

All lanes:

Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 10 µg

Lane 2:

ab302629 at 1/30 IP in Neuro-2a whole cell lysate at 10 µg

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of ab302629 in Neuro-2a whole cell lysate at 10 µg

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 150 kDa

false

Exposure time: 180s

Lab

Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (ab302629) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

Mouse brain tissue lysate at 20 µg

Lane 6:

Mouse cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 150 kDa

false

Exposure time: 20s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR26464-28

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-Fr, Flow Cyt (Intra), IP, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/2000", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "1/50", "FlowCytIntra-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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제품 세부 정보

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Part of the dynactin complex that activates the molecular motor dynein for ultra-processive transport along microtubules (By similarity). Plays a key role in dynein-mediated retrograde transport of vesicles and organelles along microtubules by recruiting and tethering dynein to microtubules. Binds to both dynein and microtubules providing a link between specific cargos, microtubules and dynein. Essential for targeting dynein to microtubule plus ends, recruiting dynein to membranous cargos and enhancing dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Can also act as a brake to slow the dynein motor during motility along the microtubule (PubMed : 25185702). Can regulate microtubule stability by promoting microtubule formation, nucleation and polymerization and by inhibiting microtubule catastrophe in neurons. Inhibits microtubule catastrophe by binding both to microtubules and to tubulin, leading to enhanced microtubule stability along the axon (PubMed : 23874158). Plays a role in metaphase spindle orientation (PubMed : 22327364). Plays a role in centriole cohesion and subdistal appendage organization and function. Its recruitment to the centriole in a KIF3A-dependent manner is essential for the maintenance of centriole cohesion and the formation of subdistal appendage. Also required for microtubule anchoring at the mother centriole (PubMed : 23386061). Plays a role in primary cilia formation (PubMed : 25774020).

See full target information DCTN1

대체 명칭 보기

Dynactin subunit 1, 150 kDa dynein-associated polypeptide, DAP-150, p135, p150-glued, DP-150, DCTN1

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DCTN1/p150-glued antibody [EPR26464-28]

Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Flow Cytometry (Intracellular) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Immunohistochemistry (Frozen sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Flow Cytometry (Intracellular) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Immunohistochemistry (Frozen sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Immunoprecipitation - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

Western blot - Anti-DCTN1/p150-glued antibody [EPR26464-28] (AB302629)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise