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AB235832

Anti-DDHD2 antibody

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Rabbit Polyclonal DDHD2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DDHD2 aa 350-650.

대체 명칭 보기

KIAA0725, SAMWD1, DDHD2, Triacylglycerol hydrolase DDHD2, TAG hydrolase, DDHD domain-containing protein 2, KIAA0725p, Phospholipase DDHD2, Triglyceride hydrolase DDHD2, Triglyceride lipase

1 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDHD2 antibody (AB235832)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDHD2 antibody (AB235832)

Immunohistochemical analysis of paraffin-embedded Human gastric cancer tissue labeling DDHD2 with ab235832 at 1/50 dilution followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Secondary antibody only control : uses 1% BSA instead of primary antibody.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human DDHD2 aa 350-650. The exact immunogen used to generate this antibody is proprietary information.

O94830

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보완 제품

Assay Kits

AB102524

Lipase Assay Kit (Colorimetric)

Functional Studies - Lipase Assay Kit (Colorimetric) (AB102524)

  • 0
  • 0
Assay Kits

AB65341

Free Fatty Acid Assay Kit - Quantification

Biochemical assay - Free Fatty Acid Assay Kit - Quantification (AB65341)

  • 5
  • 1
Primary Antibodies

AB52356

Anti-Perilipin 2 antibody

Western blot - Anti-Perilipin 2 antibody (AB52356)

  • 4
  • 3
Assay Kits

AB65336

Triglyceride Assay Kit - Quantification

Biochemical assay - Triglyceride Assay Kit - Quantification (AB65336)

  • 4
  • 5
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/500", "IHCP-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%.
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DDHD2 also known as PA-PLA1 or KIAA0725 is a phospholipase enzyme with a molecular mass of approximately 90 kDa. This enzyme plays a role in lipid metabolism by hydrolyzing phosphatidic acid. DDHD2 is expressed mainly in the brain suggesting a specialized function in neural tissue. Its enzymatic activity contributes to the dynamic regulation of lipid composition within neuronal membranes influencing membrane fluidity and signaling cascades.
Biological function summary

DDHD2 serves as a critical player in neural lipid homeostasis. It exists as a part of a larger phospholipase complex that regulates various lipid signaling pathways in neurons. The enzyme's activity affects the production of lysophosphatidic acid and other bioactive lipids impacting processes like cell signaling growth and maintenance of neuronal membranes. Loss of DDHD2 activity can therefore disrupt lipid balance affecting neuronal function.

Pathways

DDHD2 actively participates in the phospholipase signaling pathway and the endocannabinoid signaling pathway. Through its interactions DDHD2 influences these pathways by modulating lipid mediators that serve as signaling molecules. It functions along with proteins such as lipin and phospholipase C in these pathways collectively impacting membrane lipid turnover and intracellular signaling events.

DDHD2 is linked with hereditary spastic paraplegia a condition characterized by progressive spasticity and weakness of the lower limbs. Mutations in DDHD2 can lead to defective lipid metabolism in neuronal cells contributing to the pathology of this disorder. Additionally DDHD2 is connected with proteins such as spastin and atlastin which are also implicated in hereditary spastic paraplegia sharing roles in maintaining neuron structure and function.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Diacylglycerol (DAG) and triacylglycerol (TAG) lipase required for proper lipid homeostasis in the central nervous system (PubMed : 29278326, PubMed : 37832604). It cooperates with PNPLA2/ATGL in neuronal TAG catabolism and hydrolyzes sn-1,3 DAG downstream of PNPLA2/ATGL (By similarity). In vitro, it also acts as a phospholipase that hydrolyzes preferentially phosphatidic acids, including 1,2-dioleoyl-sn-phosphatidic acid, phosphatidylcholine and phosphatidylethanolamine. Specifically binds to phosphatidylinositol 3-phosphate (PI(3)P), phosphatidylinositol 4-phosphate (PI(4)P), phosphatidylinositol 5-phosphate (PI(5)P) and possibly phosphatidylinositol 4,5-bisphosphate (PI(4,5)P2). May be involved in the maintenance of the endoplasmic reticulum and/or Golgi structures. May regulate the transport between Golgi apparatus and plasma membrane.
See full target information DDHD2
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of translational medicine 21:53 PubMed36709284

2023

Upregulation of HMGB1 in tumor-associated macrophages induced by tumor cell-derived lactate further promotes colorectal cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Gao,Shiqi Zhou,Zhaofu Qin,Dechuan Li,Yuping Zhu,Dening Ma
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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