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AB50697

Anti-DDX39 antibody [2252C4a]

5

(2 리뷰들)

|

(1 출판물)

Mouse Monoclonal DDX39 antibody. Suitable for IP, WB and reacts with Human, Mouse, Recombinant fragment samples. Cited in 1 publication.
4 이미지
Immunoprecipitation - Anti-DDX39 antibody [2252C4a] (AB50697)
  • IP

Unknown

Immunoprecipitation - Anti-DDX39 antibody [2252C4a] (AB50697)

500µg HeLa cell lysate was prepared in RIPA buffer and immunoprecipitated with 5µg ab50697. The subsequent Western blot was probed with ab50697 at 1/50, followed by TrueBlot anti-mouse IgG at 1/1000. Developed by the ECL technique.

All lanes:

Immunoprecipitation - Anti-DDX39 antibody [2252C4a] (ab50697)

Predicted band size: 49 kDa

false

Western blot - Anti-DDX39 antibody [2252C4a] (AB50697)
  • WB

Unknown

Western blot - Anti-DDX39 antibody [2252C4a] (AB50697)

All lanes:

Western blot - Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution

All lanes:

HeLa whole cell lysate at 25 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 49 kDa

Observed band size: 49 kDa

true

Western blot - Anti-DDX39 antibody [2252C4a] (AB50697)
  • WB

Unknown

Western blot - Anti-DDX39 antibody [2252C4a] (AB50697)

All lanes:

Western blot - Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution

All lanes:

NIH3T3 whole cell lysate at 25 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 49 kDa

Observed band size: 49 kDa

true

Western blot - Anti-DDX39 antibody [2252C4a] (AB50697)
  • WB

Unknown

Western blot - Anti-DDX39 antibody [2252C4a] (AB50697)

All lanes:

Western blot - Anti-DDX39 antibody [2252C4a] (ab50697) at 1/100 dilution

All lanes:

F2480 whole cell lysate at 50 µg

Secondary

All lanes:

Mouse IgG antibody at 1/2500 dilution

Predicted band size: 49 kDa

Observed band size: 49 kDa

true

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

2252C4a

Isotype

IgG1

Carrier free

No

Reacts with

Mouse, Human

Applications

WB, IP

applications

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대체품

Primary Antibodies

AB225253

PE Anti-DDX39 antibody [EPR13507(B)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - PE Anti-DDX39 antibody [EPR13507(B)] (AB225253)

  • 0
  • 0
Primary Antibodies

AB225252

Alexa Fluor® 647 Anti-DDX39 antibody [EPR13507(B)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-DDX39 antibody [EPR13507(B)] (AB225252)

  • 0
  • 0
Primary Antibodies

AB180857

Anti-DDX39 antibody [EPR13508]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DDX39 antibody [EPR13508] (AB180857)

  • 5
  • 1
Primary Antibodies

AB236015

Anti-DDX39 antibody [EPR13507(B)] - BSA and Azide free

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-DDX39 antibody [EPR13507(B)] - BSA and Azide free (AB236015)

  • 0
  • 0
Primary Antibodies

AB176348

Anti-DDX39 antibody [EPR13507(B)]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-DDX39 antibody [EPR13507(B)] (AB176348)

  • 0
  • 0

보완 제품

Primary Antibodies

AB76420

Anti-RPA32/RPA2 antibody [EPR2877Y]

20ul selling size|RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2877Y] (AB76420)

  • 5
  • 1
Primary Antibodies

AB108416

Anti-HSPA2 antibody [EPR4596]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HSPA2 antibody [EPR4596] (AB108416)

  • 5
  • 1
Primary Antibodies

AB32136

Anti-Amyloid Precursor Protein antibody [Y188]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Amyloid Precursor Protein antibody [Y188] (AB32136)

  • 4
  • 9
Primary Antibodies

AB51052

Anti-Hsc70 antibody [EP1531Y]

RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Hsc70 antibody [EP1531Y] (AB51052)

  • 4
  • 2
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/100", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/100", "WB-species-notes": "<p></p>" }, "Recombinant fragment": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/50 - 1/100", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum. Filtered through a 0.22 micrometer membrane.
보관 버퍼
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 1% BSA
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
Up to 12 months
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The DDX39 protein also known as DDX39A or BAT1 belongs to the DEAD box family of proteins and has a molecular mass of approximately 49 kDa. Mechanically DDX39 functions as an RNA helicase which plays a critical role in the unwinding of RNA secondary structures during various RNA metabolic processes. The protein facilitates RNA splicing transport and translation by interacting with these fundamental cellular mechanisms. DDX39 is widely expressed in various tissues including liver heart and brain highlighting its importance across different biological systems.
Biological function summary

The multifunctional attributes of DDX39 contribute significantly to RNA processing events. The protein serves as an integral part of the TREX (transcription/export) complex which mediates the export of mRNA from the nucleus to the cytoplasm. By unwinding RNA DDX39 ensures proper mRNA maturation and transport essential for accurate gene expression. The involvement of DDX39 in the TREX complex highlights its central role in maintaining cellular homeostasis.

Pathways

DDX39 participates actively in gene expression and mRNA processing pathways. The protein's role in the TREX complex links it to the mRNA export pathway important for synthesizing proteins and cell cycle progression. Through its activities DDX39 interacts with related proteins such as ALYREF and UAP56 coordinating the transfer of mRNA. These interactions highlight its collaborative function within complex cellular processes.

DDX39 has connections to cancer including breast and lung cancer where altered mRNA processing contributes to tumor progression. The protein associates closely with other mRNA processing components like ALYREF where dysregulated interactions might influence cancer cell proliferation. Additionally DDX39 abnormalities have associations with spinal muscular atrophy where its involvement in RNA processing affects motor neuron health. This links DDX39 through its complex roles in both cancer and neurodegenerative disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Helicase that plays an essential role in mRNA export and is involved in multiple steps in RNA metabolism including alternative splicing (PubMed : 33941617, PubMed : 38801080). Regulates nuclear mRNA export to the cytoplasm through association with ECD (PubMed : 33941617). Also involved in spliceosomal uridine-rich small nuclear RNA (U snRNA) export by stimulating the RNA binding of adapter PHAX (PubMed : 39011894). Plays a role in the negative regulation of type I IFN production by increasing the nuclear retention of antiviral transcripts and thus reducing their protein expression (PubMed : 32393512). Independently of the interferon pathway, plays an antiviral role against alphaviruses by binding to a 5' conserved sequence element in the viral genomic RNA (PubMed : 37949067).
See full target information DDX39A

대체 명칭 보기

DDX39, DDX39A, ATP-dependent RNA helicase DDX39A, DEAD box protein 39, Nuclear RNA helicase URH49
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cancer science 103:1363-9 PubMed22494014

2012

DDX39 acts as a suppressor of invasion for bladder cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Minoru Kato,Min Wei,Shotaro Yamano,Anna Kakehashi,Satoshi Tamada,Tatsuya Nakatani,Hideki Wanibuchi
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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