AB248528

Anti-DDX50 antibody [EPR5272] - BSA and Azide free

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal DDX50 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

ATP-dependent RNA helicase DDX50, DEAD box protein 50, Gu-beta, Nucleolar protein Gu2, DDX50

2 이미지

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Western blot - Anti-DDX50 antibody [EPR5272] - BSA and Azide free (AB248528)

This data was developed using ab133507, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-DDX50 antibody [EPR5272] (<a href='/ko/products/primary-antibodies/ddx50-antibody-epr5272-ab133507'>ab133507</a>) at 1/50000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

K562 cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat-anti-rabbit HRP at 1/2000 dilution

Predicted band size: 83 kDa

Observed band size: 83 kDa

false

OI-RD Scanning - Anti-DDX50 antibody [EPR5272] - BSA and Azide free (AB248528)

OI-RD Scanning

Unknown

OI-RD Scanning - Anti-DDX50 antibody [EPR5272] - BSA and Azide free (AB248528)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR5272

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

ab248528 is the carrier-free version of ab133507.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

보관 정보

Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DDX50 also known as DEAD box polypeptide 50 is an RNA helicase with ATPase activity that unwinds RNA functioning in ribosome biogenesis. It belongs to the DEAD-box protein family characterized by the conserved motif Asp-Glu-Ala-Asp (D-E-A-D). The molecular weight of DDX50 is approximately 82 kDa. This protein expresses mainly in nucleoli within cells where it likely participates in pre-rRNA processing and ribosomal subunit assembly.

Biological function summary

The protein DDX50 plays roles in RNA processing and modification during ribosome production. It does not function alone but as part of a ribonucleoprotein complex that facilitates the maturation of ribosomal RNA. By interacting with other components it assists in the structural rearrangements and modifications necessary for proper ribosome assembly impacting overall cellular protein synthesis.

Pathways

Cellular processes involving ribosome assembly and function depend on proteins like DDX50. It integrates in the ribosome biogenesis pathway influencing the synthesis and assembly of ribosomal subunits. In this pathway DDX50 works with proteins such as DDX21 and NOP56 sharing functional domains to facilitate efficient rRNA modification and processing which are critical to ribosome construction and cellular growth.

Alterations in DDX50 expression or function connect to cancer and some hematological conditions. DDX50's role in ribosome biogenesis makes it a factor in tumorigenesis particularly in cancers where cell proliferation relies heavily on increased protein synthesis. Additionally DDX50 interaction with proteins like NOP56 points to a potential link to dyskeratosis congenita a disorder affecting bone marrow and the skin. Understanding these connections may open doors for developing diagnostic or therapeutic strategies targeting DDX50 and similar proteins.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

ATP-dependent RNA helicase that may play a role in various aspects of RNA metabolism including pre-mRNA splicing or ribosomal RNA production (PubMed : 12027455). Also acts as a viral restriction factor and promotes the activation of the NF-kappa-B and IRF3 signaling pathways following its stimulation with viral RNA or infection with RNA and DNA viruses (PubMed : 35215908). For instance, decreases vaccinia virus, herpes simplex virus, Zika virus or dengue virus replication during the early stage of infection (PubMed : 28181036, PubMed : 35215908). Mechanistically, acts via the adapter TICAM1 and independently of the DDX1-DDX21-DHX36 helicase complex to induce the production of interferon-beta (PubMed : 35215908).

See full target information DDX50

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Psychiatry investigation 22:699-713 PubMed40566894

2025

Transcriptional Landscape and Biomarker Discovery for Endoplasmic Reticulum Stress in Alzheimer's Disease: An Ex Vivo Study Using Patients-Derived Dermal Fibroblasts.

Applications

Unspecified application

Species

Unspecified reactive species

Yeojin Kim,You Jin Nam,Sunwoo Yoon,Young Joon Cho,Ho Min Song,Seongmin Kim,Donghyuk Shin,Jin Young Noh,Sun Min Lee,So Young Moon,Eun-Joo Kim,Soo Hyun Cho,Byeong C Kim,Seong Hye Choi,Sang Won Seo,Jin Wook Choi,Young-Sil An,Bumhee Park,Young Joon Park,Hee Young Kang,Hyun Goo Woo,Yong Hyuk Cho,Sunhwa Hong,Sang Joon Son,Sang-Rae Lee,Chang Hyung Hong,Hyun Woong Roh

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DDX50 antibody [EPR5272] - BSA and Azide free

Western blot - Anti-DDX50 antibody [EPR5272] - BSA and Azide free (AB248528)

OI-RD Scanning - Anti-DDX50 antibody [EPR5272] - BSA and Azide free (AB248528)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise