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AB325347

Anti-DECTIN 2 antibody [EPR30285-562]

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Rabbit Recombinant Monoclonal DECTIN 2 antibody. Suitable for Flow Cyt and reacts with Mouse samples.
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Flow Cytometry - Anti-DECTIN 2 antibody [EPR30285-562] (AB325347)
  • Flow Cyt

Lab

Flow Cytometry - Anti-DECTIN 2 antibody [EPR30285-562] (AB325347)

Flow cytometric analysis of Mouse bone marrow cells labelling DECTIN 2 with ab325347 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD3 conjugated to Alexa Fluor®488.

Flow Cytometry - Anti-DECTIN 2 antibody [EPR30285-562] (AB325347)
  • Flow Cyt

Lab

Flow Cytometry - Anti-DECTIN 2 antibody [EPR30285-562] (AB325347)

Flow cytometric analysis of Mouse bone marrow cells labelling DECTIN 2 with ab325347 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat anti-Rabbit IgG (Alexa Fluor® 647, ab150083) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells. Cells were co-stained with anti-CD11b conjugated to Brilliant Violet 421.

Flow Cytometry - Anti-DECTIN 2 antibody [EPR30285-562] (AB325347)
  • Flow Cyt

Lab

Flow Cytometry - Anti-DECTIN 2 antibody [EPR30285-562] (AB325347)

Flow cytometric analysis of Mouse bone marrow derived Dendritic cells induced by 20ng/ml GM-CSF for 13 days and 50ng/ml IL4 for 7 days (Lower left and right) Mouse bone marrow (Upper left and right) cells labelling DECTIN 2 with ab325347 at 1/500 dilution (0.1ug) / Upper right and Lower right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable lymphoid cells. Cells were co-stained with anti-CD11c conjugated to Brilliant Violet 421.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30285-562

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

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  • 19
Primary Antibodies

AB133616

Anti-CD4 antibody [EPR6855]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

  • 5
  • 20
Proteins & Peptides

AB276804

Recombinant Mouse DECTIN 2 protein (His tag)

SDS-PAGE - Recombinant Mouse DECTIN 2 protein (His tag) (AB276804)

  • 0
  • 0
Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/500", "FlowCyt-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
배송 시 보관 조건
Conditional Ambient
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Calcium-dependent lectin that acts as a pattern recognition receptor (PRR) of the innate immune system : specifically recognizes and binds alpha-mannans on C.albicans hypheas (PubMed : 17050534, PubMed : 19703985, PubMed : 20493731). Binding of C.albicans alpha-mannans to this receptor complex leads to phosphorylation of the immunoreceptor tyrosine-based activation motif (ITAM) of FCER1G, triggering activation of SYK, CARD9 and NF-kappa-B, consequently driving maturation of antigen-presenting cells and shaping antigen-specific priming of T-cells toward effector T-helper 1 and T-helper 17 cell subtypes (PubMed : 17050534, PubMed : 19703985, PubMed : 20493731, PubMed : 32358020). Also recognizes, in a mannose-dependent manner, allergens from house dust mite and fungi, by promoting cysteinyl leukotriene production (PubMed : 19124755). Recognizes soluble elements from the eggs of Shistosoma mansoni altering adaptive immune responses (PubMed : 21059925).
See full target information Clec6a

대체 명칭 보기

Clec4n, Clecsf10, Dectin2, Clec6a, C-type lectin domain family 6 member A, C-type lectin superfamily member 10, Dendritic cell-associated C-type lectin 2, DC-associated C-type lectin 2, Dectin-2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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