Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205]

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (AB32403)

ab32403 at a 1/50 dilution staining delta 1 Catenin/CAS in paraffin embedded breast carcinoma tissue sections by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Unknown

Western blot - Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (AB32403)

All lanes:

Western blot - Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (ab32403)

Lane 1:

HeLa cell lysate, untreated

Lane 2:

HeLa cell lysate, treated with Pervanandate

Lane 3:

HeLa cell lysate, treated with Phosphatase

Predicted band size: 108 kDa

Observed band size: 115 kDa

false

• WB

CiteAb

Western blot - Anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] (AB32403)

delta 1 Catenin/CAS (phospho Y228) western blot using anti-delta 1 Catenin/CAS (phospho Y228) antibody [E205] ab32403. Publication image and figure legend from Park, S. Y., Yoon, S., et al., 2017, Int J Mol Sci, PubMed 29207493.

ab32403 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab32403 please see the product overview.

90K interacts with E-cadherin, which affects the phosphorylation status of p120-catenin and thereby decreases the p120-catenin/E-cadherin association. (a) 90K was co-immunoprecipitated with E-cadherin and p120-catenin, but not with β-catenin. 293T cells were either transfected or treated with 90K (left panel) or 90K conditioned media (90K/CM) (right panel), followed by immunoprecipitation assay with an anti-myc antibody; (b) 90K/CM treatment decreased the interaction between p120-catenin and E-cadherin. 293T cells were treated with either ctrl/CM or 90K/CM for 16 h and immunoprecipitated with an anti-p120-catenin antibody; (c) Addition of 90K in 293T cell lysate decreased the interaction between p120-catenin and E-cadherin in vitro. Immunoprecipitation assay was performed by anti-p120-catenin antibody using 293T cell lysates added with or without 90K; (d) Knockdown of p120-catenin did not significantly affect the interaction between 90K and E-cadherin. 293T cells were transfected with 90K together with p120-catenin short hairpin RNA (shRNA), which is specific for either canine (C) or human (H) p120-catenin. The shRNA targeting canine p120-catenin, which cannot bind to human p120-catenin, was used as a negative control; (e) 90K/CM treatment affected the phosphorylation status of p120-catenin. 293T cells were treated with either ctrl/CM or 90K/CM for 16 h. p120-Catenin immuno-complex beads were followed by western blot analysis using antibodies against phospho-Ser, phospho-Thr, or phospho-Tyr; (f) 90K/CM treatment decreased phosphorylation of p120-catenin at Tyr-228 residue. 293T cells were treated with either ctrl/CM or 90K/CM for 16 h, and immunoblotted for phospho-Tyr-228-p120-catenin, p120-catenin, E-cadherin, and actin.

false