AB240596

Anti-DENTT antibody

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Rabbit Polyclonal DENTT antibody. Suitable for IP, WB, IHC-P and reacts with Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human TSPYL2 aa 1-50.

대체 명칭 보기

CDA1, DENTT, TSPX, HRIHFB2216, TSPYL2, Testis-specific Y-encoded-like protein 2, TSPY-like protein 2, Cell division autoantigen 1, Cutaneous T-cell lymphoma-associated antigen se20-4, Differentially-expressed nucleolar TGF-beta1 target protein, Nuclear protein of 79 kDa, CTCL-associated antigen se20-4, NP79

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENTT antibody (AB240596)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENTT antibody (AB240596)

Formalin-fixed, paraffin-embedded human lung cancer tissue stained for DENTT using ab240596 at 1/1000 dilution in immunohistochemical analysis. DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENTT antibody (AB240596)

Formalin-fixed, paraffin-embedded human testis tissue stained for DENTT using ab240596 at 1/1000 dilution in immunohistochemical analysis. DAB staining.

Supplier Data

Immunoprecipitation - Anti-DENTT antibody (AB240596)

DENTT was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP; 20% of IP loaded) using ab240596 at 6 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab240596 at 1 μg/mL.

Lane 1 : ab240596 IP in HeLa whole cell lysate.
Lane 2 : Control IgG IP in HeLa whole cell lysate.

Detection : Chemiluminescence with an exposure time of 30 seconds.

All lanes:

Immunoprecipitation - Anti-DENTT antibody (ab240596)

Predicted band size: 79 kDa

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Supplier Data

Western blot - Anti-DENTT antibody (AB240596)

All lanes:

Western blot - Anti-DENTT antibody (ab240596) at 0.4 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 79 kDa

true

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP, IHC-P

applications

Immunogen

Synthetic Peptide within Human TSPYL2 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9H2G4

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/2000", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DENTT also known as DEP domain-containing protein 5 (DEPDC5) is a member of the GATOR complex. It has a molecular mass of approximately 160 kDa. DENTT is expressed in various human tissues with high levels in the brain and skeletal muscle. This protein plays a role in the mechanisms of nutrient sensing and detecting energy levels within cells.

Biological function summary

DENTT regulates the activity of the mechanistic target of rapamycin complex 1 (mTORC1). As part of the GATOR1 complex it serves to inhibit mTORC1 in response to nutrient levels. By doing so DENTT participates in cellular processes such as growth and metabolism. Its activity is linked to the control of cell size and autophagy.

Pathways

DENTT is involved in the mTOR signaling pathway an important regulator of cell growth and proliferation. It interacts with proteins such as NPRL2 and NPRL3 which form part of the same GATOR1 complex. The interplay between DENTT and these proteins modulates mTORC1 activity aligning cellular metabolism with nutrient availability.

Alterations in DENTT are implicated in familial focal epilepsies. Mutations within the DEPDC5 gene can disturb mTOR signaling leading to imbalanced neuronal excitability. Additionally aberrant DENTT activity connects with proteins like RHEB known to drive mTORC1 activity which contributes to the pathogenesis of certain epileptic conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Part of the CASK/TBR1/TSPYL2 transcriptional complex which modulates gene expression in response to neuronal synaptic activity, probably by facilitating nucleosome assembly. May inhibit cell proliferation by inducing p53-dependent CDKN1A expression.

See full target information TSPYL2

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 16:271 PubMed40210616

2025

Role of METTL16 in PPARγ methylation and osteogenic differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Liangjie Lu,Lijun Wang,Minjie Yang,Huihan Wang

Heliyon 10:e39260 PubMed39669138

2024

Systematic exploration of the molecular characteristics of CD8 T cells to predict the response to immunotherapy and the prognosis of patients with colon adenocarcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Shen,Lifeng Shang,Junwei Han,Yi Zhang,Wenkai Niu,Haiwang Liu,Hai Shi

Frontiers in immunology 14:1077003 PubMed37033917

2023

Single-cell protein activity analysis reveals a novel subpopulation of chondrocytes and the corresponding key master regulator proteins associated with anti-senescence and OA progression.

Applications

Unspecified application

Species

Unspecified reactive species

Zhao Guang,Zhang Min,Li Jun-Tan,Dou Tian-Xu,Gao Xiang

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DENTT antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENTT antibody (AB240596)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DENTT antibody (AB240596)

Immunoprecipitation - Anti-DENTT antibody (AB240596)

Western blot - Anti-DENTT antibody (AB240596)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise