AB8470

Anti-Desmin antibody [D33]

Name: Anti-Desmin antibody [D33]
Brand: Abcam Limited
SKU: AB8470
Rating: 4 (6 reviews)

BOND RX™ Validated
각 태그의 뜻

(6 리뷰들)

(49 제품이 사용된 논문 )

Mouse Monoclonal Desmin antibody. Suitable for IHC-P and reacts with Human samples. Cited in 49 publications. Immunogen corresponding to Tissue preparation containing DES protein.

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [D33] (AB8470)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [D33] (AB8470)

IHC image of ab8470 staining in human skeletal muscle formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab8470, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

D33

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Tissue preparation containing DES protein. The exact immunogen used to generate this antibody is proprietary information.

P17661

Specificity

This antibody is highly reactive with desmin. On immunoblots only the 56 kD desmin band is stained. Non reactive with GFAP, Keratin, Vimentin and Neurofilament.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" } } }

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제품 세부 정보

Sufficient antibody for 100-500 tests

This antibody stains muscle cells in human tissues

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특성 및 보관 정보

제형

Liquid

Purity

Tissue culture supernatant

보관 버퍼

pH: 7.3 Preservative: 0.065% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Desmin also referred to as Desmin 20 is an intermediate filament protein with a molecular weight of approximately 53 kDa. Primarily found in muscle cells desmin provides structural integrity to cells being an important component of the cytoskeleton. Desmin expression is highly specific to cardiac skeletal and smooth muscle tissues and it can be detected using desmin marker techniques like desmin IHC (Immunohistochemistry). Desmin staining or desmin stain methods are commonly employed in diagnostic labs to visualize this protein within tissue samples.

Biological function summary

Desmin plays a significant role in maintaining cellular architecture by anchoring organelles such as nuclei and mitochondria within the cytoplasm. It associates with other proteins to form desmin intermediate filament networks which facilitate force transmission and maintain mechanical integrity. This protein does not operate in isolation but is part of a larger complex of cytoskeletal elements that reinforce muscle cell shape and resilience. Its interactions and binding with other cytoskeletal components confirm its essential role in muscle structure.

Pathways

Desmin links closely to the cell structure and function pathways providing stability during muscle contraction processes. It interacts with other proteins including actin and tubulin within the cytoskeleton network ensuring coordinated responses to mechanical stress. Specifically desmin integrates into pathways related to the maintenance of the sarcomere structure thereby connecting to additional important proteins like myosin and dystrophin which collectively uphold muscular function and integrity.

Desmin mutations can lead to myopathies such as desmin-related myopathy (DRM) or cardiomyopathy where the structural disruptions result in abnormal muscle function. These conditions typically manifest as muscle weakness or cardiac abnormalities. Furthermore its relationship to the sarcomere suggests interactions with proteins like dystrophin in muscular dystrophies where desmin integrity critically influences disease progression and severity. Understanding desmin's link to such disorders underpins its role as a diagnostic marker and a potential therapeutic target in muscle-related diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Muscle-specific type III intermediate filament essential for proper muscular structure and function. Plays a crucial role in maintaining the structure of sarcomeres, inter-connecting the Z-disks and forming the myofibrils, linking them not only to the sarcolemmal cytoskeleton, but also to the nucleus and mitochondria, thus providing strength for the muscle fiber during activity (PubMed : 25358400). In adult striated muscle they form a fibrous network connecting myofibrils to each other and to the plasma membrane from the periphery of the Z-line structures (PubMed : 24200904, PubMed : 25394388, PubMed : 26724190). May act as a sarcomeric microtubule-anchoring protein : specifically associates with detyrosinated tubulin-alpha chains, leading to buckled microtubules and mechanical resistance to contraction. Required for nuclear membrane integrity, via anchoring at the cell tip and nuclear envelope, resulting in maintenance of microtubule-derived intracellular mechanical forces (By similarity). Contributes to the transcriptional regulation of the NKX2-5 gene in cardiac progenitor cells during a short period of cardiomyogenesis and in cardiac side population stem cells in the adult. Plays a role in maintaining an optimal conformation of nebulette (NEB) on heart muscle sarcomeres to bind and recruit cardiac alpha-actin (By similarity).

See full target information DES

대체 명칭 보기

Desmin, DES

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제품이 사용된 논문 (49)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 15:12719 PubMed40223147

2025

Controlled delivery of PRP from decellularized extracellular matrix enhances skeletal muscle regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Saeideh Erfanian,Farhad Mostafaei,Fatemeh Ajalloueian,Hossein Baharvand,Sarah Rajabi,Mohammad Kazemi Ashtiani

Foods (Basel, Switzerland) 13: PubMed39200458

2024

Hydroxyl Radical-Induced Oxidation on the Properties of Cathepsin H and Its Influence in Myofibrillar Proteins Degradation of .

Applications

Unspecified application

Species

Unspecified reactive species

Xuemei Fan,Mengjie Ma,Pingping Liu,Xiaorong Deng,Jian Zhang

iScience 27:109930 PubMed38832025

2024

Immortalized human myotonic dystrophy type 1 muscle cell lines to address patient heterogeneity.

Applications

Unspecified application

Species

Unspecified reactive species

Judit Núñez-Manchón,Júlia Capó,Alicia Martínez-Piñeiro,Eduard Juanola,Jovan Pesovic,Laura Mosqueira-Martín,Klaudia González-Imaz,Pau Maestre-Mora,Renato Odria,Estefania Cerro-Herreros,Neia Naldaiz-Gastesi,Adolfo López de Munain,Rubén Artero,Dusanka Savic-Pavicevic,Ainara Vallejo-Illarramendi,Kamel Mamchaoui,Anne Bigot,Vincent Mouly,Mònica Suelves,Gisela Nogales-Gadea

Acta neuropathologica communications 12:80 PubMed38790073

2024

Aberrant myonuclear domains and impaired myofiber contractility despite marked hypertrophy in MYMK-related, Carey-Fineman-Ziter Syndrome.

Applications

Unspecified application

Species

Unspecified reactive species

Hannah F Dugdale,Yotam Levy,Heinz Jungbluth,Anders Oldfors,Julien Ochala

Biological research 57:29 PubMed38760841

2024

Therapeutic potential of oleic acid supplementation in myotonic dystrophy muscle cell models.

Applications

Unspecified application

Species

Unspecified reactive species

Nerea Moreno,Maria Sabater-Arcis,Teresa Sevilla,Manuel Perez Alonso,Jessica Ohana,Ariadna Bargiela,Ruben Artero

International journal of molecular sciences 25: PubMed38674157

2024

Role of Protein Tyrosine Phosphatase Receptor Type E (PTPRE) in Chemoresistant Retinoblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Lars Mohren,Annika Doege,Natalia Miroschnikov,Oliver Dräger,Maike Anna Busch,Nicole Dünker

Molecules (Basel, Switzerland) 28: PubMed37446781

2023

Oxidation of Cathepsin D by Hydroxy Radical: Its Effect on Enzyme Structure and Activity against Myofibrillar Proteins Extracted from .

Applications

Unspecified application

Species

Unspecified reactive species

Mengjie Ma,Pingping Liu,Chaoye Wang,Xiaorong Deng,Lianfu Zhang,Jian Zhang

NPJ Regenerative medicine 8:25 PubMed37217533

2023

A superior extracellular matrix binding motif to enhance the regenerative activity and safety of therapeutic proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Yasmin K Alshoubaki,Yen-Zhen Lu,Julien M D Legrand,Rezvan Karami,Mathilde Fossat,Ekaterina Salimova,Ziad Julier,Mikaël M Martino

BMC molecular and cell biology 24:18 PubMed37189080

2023

Myogenic differentiation of human myoblasts and Mesenchymal stromal cells under GDF11 on NPoly-ɛ-caprolactone-collagen I-Polyethylene-nanofibers.

Applications

Unspecified application

Species

Unspecified reactive species

Aijia Cai,Paul Schneider,Zeng-Ming Zheng,Justus P Beier,Marcus Himmler,Dirk W Schubert,Volker Weisbach,Raymund E Horch,Andreas Arkudas

Cancer cell international 23:34 PubMed36841751

2023

Chorioallantoic membrane assay revealed the role of TIPARP (2,3,7,8-tetrachlorodibenzo-p-dioxin-inducible poly (ADP-ribose) polymerase) in lung adenocarcinoma-induced angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Kenji Miura,Michiyo Koyanagi-Aoi,Yoshimasa Maniwa,Takashi Aoi

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmin antibody [D33]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmin antibody [D33] (AB8470)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purity

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (49)

Product promise