AB218849

Anti-Desmocollin 2/3 antibody [7G6] - Extracellular domain - BSA and Azide free

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Mouse Monoclonal Desmocollin 2 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human DSC2.

1 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmocollin 2/3 antibody [7G6] - Extracellular domain - BSA and Azide free (AB218849)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmocollin 2/3 antibody [7G6] - Extracellular domain - BSA and Azide free (AB218849)

Immunohistochemical analysis of formalin-fixed paraffin-embedded human skin tissue sections labeling Desmocollin 2/3 with ab218849 at 2 μg/mL.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

7G6

Isotype

IgG1

Light chain 타입

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DSC2. The exact immunogen used to generate this antibody is proprietary information.

Q02487

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1-2 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

pH: 7.2 - 7.4 Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Desmocollin 2 also known as DSC2 is a cadherin protein and an important component of desmosomes which are structures that facilitate cell-cell adhesion in epithelial tissues. It has a molecular weight of approximately 100 kDa. This protein expresses strongly in tissues rich in desmosomes such as the epidermis and cardiac tissue. Desmocollin 2 stabilizes cells against shearing forces ensuring that they adhere tightly by interacting with other desmosomal proteins.

Biological function summary

Desmocollin 2 plays a role in maintaining tissue integrity and cohesion. It participates as a part of the desmosomal complex where it interacts with other cadherin family members like desmogleins and anchors to the intermediate filaments inside the cell. These interactions help to maintain the architecture and structural support during mechanical stress especially in tissues undergoing constant stress like the heart and skin.

Pathways

Desmocollin 2's mechanical role intersects with various cell signaling pathways. Notably it plays a role in the Wnt signaling pathway which is important for cell proliferation and differentiation. The protein also interacts with plakoglobin a protein involved in beta-catenin regulation within these pathways. Desmocollin 2's involvement in cell adhesion and signaling nodes suggests its contribution to further complex signaling cascades.

Desmocollin 2 mutations and disruptions associate with arrhythmogenic right ventricular cardiomyopathy (ARVC) and certain skin blistering conditions. In ARVC the weakened cell adhesion due to desmocollin 2 defects can lead to fibrofatty replacement of myocardium impacting cardiac function. The protein plakoglobin often appears as a related factor in these diseases as both proteins interact at the desmosomal junctions and contribute to cell adhesion integrity.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 33596089). Promotes timely incorporation of DSG2 into desmosome intercellular junctions and promotes interaction of desmosome cell junctions with intermediate filament cytokeratin, via modulation of DSP phosphorylation (PubMed : 33596089). Plays an important role in desmosome-mediated maintenance of intestinal epithelial cell intercellular adhesion strength and barrier function (PubMed : 33596089). Positively regulates wound healing of intestinal mucosa via promotion of epithelial cell migration, and also plays a role in mechanotransduction of force between intestinal epithelial cells and extracellular matrix (PubMed : 31967937). May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. May promote p38MAPK signaling activation that facilitates keratinocyte migration (By similarity).

See full target information DSC2

Additional targets

DSC3

대체 명칭 보기

CDHF2, DSC3, DSC2, Desmocollin-2, Cadherin family member 2, Desmocollin-3, Desmosomal glycoprotein II, Desmosomal glycoprotein III, CDHF3, DSC4, DSC3, Desmocollin-3, Cadherin family member 3, Desmocollin-4, HT-CP

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록and refine your search

Parasitology 148:341-353 PubMed33100232

2020

infections induce G cell cycle arrest and a senescence-like phenotype in endothelial host cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zahady D Velásquez,Sara López-Osorio,Daniel Waiger,Carolina Manosalva,Learta Pervizaj-Oruqaj,Susanne Herold,Carlos Hermosilla,Anja Taubert

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmocollin 2/3 antibody [7G6] - Extracellular domain - BSA and Azide free

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmocollin 2/3 antibody [7G6] - Extracellular domain - BSA and Azide free (AB218849)

주요 정보

Host species

Clonality

Clone number

Isotype

Light chain 타입

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Additional targets

대체 명칭 보기

제품이 사용된 논문 (1)

Product promise