AB95967

Anti-Desmocollin 2 antibody

Name: Anti-Desmocollin 2 antibody
Brand: Abcam Limited
SKU: AB95967
Rating: 4 (2 reviews)

(2 리뷰들)

(6 제품이 사용된 논문 )

Rabbit Polyclonal Desmocollin 2 antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DSC2 aa 450 to C-terminus.

2 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmocollin 2 antibody (AB95967)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmocollin 2 antibody (AB95967)

Paraffin embedded OVCAR3 xenograft tissue stained for Desmocollin 2 using ab95967 at 1/500 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-Desmocollin 2 antibody (AB95967)

7.5% SDS PAGE

All lanes:

Western blot - Anti-Desmocollin 2 antibody (ab95967) at 1/500 dilution

All lanes:

A431 whole cell lysate at 30 µg

Predicted band size: 99 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human DSC2 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q02487

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Desmocollin 2 also known as DSC2 is a cadherin protein and an important component of desmosomes which are structures that facilitate cell-cell adhesion in epithelial tissues. It has a molecular weight of approximately 100 kDa. This protein expresses strongly in tissues rich in desmosomes such as the epidermis and cardiac tissue. Desmocollin 2 stabilizes cells against shearing forces ensuring that they adhere tightly by interacting with other desmosomal proteins.

Biological function summary

Desmocollin 2 plays a role in maintaining tissue integrity and cohesion. It participates as a part of the desmosomal complex where it interacts with other cadherin family members like desmogleins and anchors to the intermediate filaments inside the cell. These interactions help to maintain the architecture and structural support during mechanical stress especially in tissues undergoing constant stress like the heart and skin.

Pathways

Desmocollin 2's mechanical role intersects with various cell signaling pathways. Notably it plays a role in the Wnt signaling pathway which is important for cell proliferation and differentiation. The protein also interacts with plakoglobin a protein involved in beta-catenin regulation within these pathways. Desmocollin 2's involvement in cell adhesion and signaling nodes suggests its contribution to further complex signaling cascades.

Desmocollin 2 mutations and disruptions associate with arrhythmogenic right ventricular cardiomyopathy (ARVC) and certain skin blistering conditions. In ARVC the weakened cell adhesion due to desmocollin 2 defects can lead to fibrofatty replacement of myocardium impacting cardiac function. The protein plakoglobin often appears as a related factor in these diseases as both proteins interact at the desmosomal junctions and contribute to cell adhesion integrity.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

A component of desmosome cell-cell junctions which are required for positive regulation of cellular adhesion (PubMed : 33596089). Promotes timely incorporation of DSG2 into desmosome intercellular junctions and promotes interaction of desmosome cell junctions with intermediate filament cytokeratin, via modulation of DSP phosphorylation (PubMed : 33596089). Plays an important role in desmosome-mediated maintenance of intestinal epithelial cell intercellular adhesion strength and barrier function (PubMed : 33596089). Positively regulates wound healing of intestinal mucosa via promotion of epithelial cell migration, and also plays a role in mechanotransduction of force between intestinal epithelial cells and extracellular matrix (PubMed : 31967937). May contribute to epidermal cell positioning (stratification) by mediating differential adhesiveness between cells that express different isoforms. May promote p38MAPK signaling activation that facilitates keratinocyte migration (By similarity).

See full target information DSC2

대체 명칭 보기

CDHF2, DSC3, DSC2, Desmocollin-2, Cadherin family member 2, Desmocollin-3, Desmosomal glycoprotein II, Desmosomal glycoprotein III

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제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology 27: PubMed40879390

2025

Comprehensive analysis of desmosomal protein remodelling identifies desmocollin-2 as potential biomarker for arrhythmogenic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Jie Ren,Zhenliang Hu,Lingmin Wu,Deniz Akdis,Weiquan Ye,Ardan M Saguner,Mingming Su,Hongbin Dong,Zhongli Chen,Dan Hu,Shengshou Hu,Firat Duru,Liang Chen

Journal of Cancer 14:2619-2632 PubMed37779876

2023

Validation the role of desmocollin-2 in osteosarcoma based on single cell and bulk RNA seq and experimental analyses.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaxing Zeng,Yu Sun,Yunan Man,Haijun Tang,Long Xie,Maolin He

International journal of molecular sciences 24: PubMed37446408

2023

EPs 7630 Stimulates Tissue Repair Mechanisms and Modifies Tight Junction Protein Expression in Human Airway Epithelial Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Fang,Liang Zhou,Žarko Kulić,Martin D Lehner,Michael Tamm,Michael Roth

Frontiers in nutrition 7:628405 PubMed33521042

2021

LAT1 Protein Content Increases Following 12 Weeks of Resistance Exercise Training in Human Skeletal Muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Paul A Roberson,C Brooks Mobley,Matthew A Romero,Cody T Haun,Shelby C Osburn,Petey W Mumford,Christopher G Vann,Rory A Greer,Arny A Ferrando,Michael D Roberts

Experimental and toxicologic pathology : official 66:437-44 PubMed25153024

2014

Immunohistological characterization of intercellular junction proteins in rhesus macaque intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Sanjeev Gumber,Asma Nusrat,Francois Villinger

The Journal of biological chemistry 286:1499-507 PubMed21071449

2010

Desmosome assembly and cell-cell adhesion are membrane raft-dependent processes.

Applications

Species

Dog

Natasa Resnik,Kristina Sepcic,Ana Plemenitas,Reinhard Windoffer,Rudolf Leube,Peter Veranic

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Desmocollin 2 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Desmocollin 2 antibody (AB95967)

Western blot - Anti-Desmocollin 2 antibody (AB95967)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise