AB254154

Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control

Name: Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control
Brand: Abcam Limited
SKU: AB254154
Rating: 5 (1 reviews)

BOND RX™ Validated
Recombinant
Lab Essentials
각 태그의 뜻

(1 리뷰)

(5 제품이 사용된 논문 )

Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154) is a mouse monoclonal antibody detecting Detyrosinated alpha Tubulin in Western Blot, IHC-P, IHC-Fr, ICC/IF. Suitable for Human, Mouse, Rat.

8 이미지

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Immunohistochemical analysis of paraffin-embedded human cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on human cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on mouse cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/100 dilution followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 (2μg/ml) dilution (Green). Cytoplasmic staining on rat cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 (2μg/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized mouse primary neuron cells labelling Detyrosinated A Tubulin with ab254154 at 1/250 dilution, followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in mouse primary neuron cells. Confocal scanning Z step was set as 0.3μm followed by image processing with maximum Z projection. ab179513 Anti-beta Tubulin rabbit monoclonal antibody was used to counterstain tubulin at 1/200 dilution, followed by ab150080 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 594) (Red). The nuclear counterstain was DAPI (Blue).

IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/100 dilution followed by ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 (2μg/ml) dilution (Green). Cytoplasmic staining on mouse cerebrum is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150113 Goat Anti-mouse IgG H&L (Alexa Fluor^® 488) at 1/1000 (2μg/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue labeling Detyrosinated A Tubulin with ab254154 at 1/4000 dilution followed by ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879). Cytoplasmic staining on rat cerebrum. The section was incubated with ab254154 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is ready to use Goat Anti-mouse IgG H&L (HRP polymer) (ab214879).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Lab

Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure times : Lane 1, 2 : 3 seconds; Lane 3, 4 : 7 seconds.

false

Unknown

Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Blocking/Dilution buffer : 5% NFDM/TBST.

Negative control : Mouse heart (PMID : 15899979).

false

Exposure time: 7s

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

AA12

Isotype

IgG2a

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-Fr, IHC-P, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "Not recommended for human ICC/IF.", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "Not recommended for human ICC/IF.", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/4000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." } } }

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제품 세부 정보

What is this antibody validated in?
Anti-Detyrosinated alpha Tubulin antibody [AA12] - Microtubule Marker (ab254154) is a mouse recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Mouse, Rat samples.

What is the molecular weight of Detyrosinated alpha Tubulin?
Anti-Detyrosinated alpha Tubulin [AA12] - Microtubule Marker (ab254154) specifically detects a band for Detyrosinated alpha Tubulin (UniProt: Q71U36) at a molecular weight of 50kDa.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Detyrosinated alpha Tubulin also known as detyrosinated tubulin or alpha tubulin is a modified form of the alpha subunit of tubulin. This protein undergoes detyrosination which entails removing the terminal tyrosine residue. Detyrosinated alpha Tubulin has an approximate molecular mass of 50 kDa. It localizes mainly in the cytoplasm and plays an important role in the stability and function of microtubules which are key components of the cytoskeleton. These modifications regulate microtubules' dynamic properties impacting cellular processes such as intracellular transport and cell division.

Biological function summary

Detyrosinated alpha Tubulin impacts important cellular functions. It is an integral part of the microtubule network. Microtubules provide structural support to the cell aiding in intracellular transport and cell shape maintenance. The detyrosination of alpha tubulin contributes to the regulation of microtubule stability which is essential for accurate chromosome segregation during mitosis. Furthermore the detyrosination influences interactions with microtubule-associated proteins and motor proteins affecting their binding affinity and activity.

Pathways

Detyrosinated alpha Tubulin is significant in cellular signaling and transport. It is an important part of the microtubule-dependent transport pathways that facilitate the movement of cellular components including vesicles and organelles. The detyrosination of alpha tubulin influences its interaction with motor proteins such as kinesins and dyneins which are responsible for directional transport along microtubules. Additionally alterations in tubulin detyrosination affect the Wnt signaling pathway impacting cellular processes such as proliferation and differentiation.

Alterations in the detyrosination of alpha Tubulin have links to neurodegenerative diseases and cancer. In neurodegenerative disorders abnormal detyrosination can disrupt axonal transport contributing to diseases like Alzheimer's. In cancer increased levels of detyrosinated alpha Tubulin can enhance cell invasiveness and metastasis. The dysregulation of tubulin detyrosination affects cellular interactions with proteins such as tau in neurodegenerative disorders and beta-catenin in cancer implicating these proteins in disease progression.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

See full target information TUBA1A

대체 명칭 보기

TUBA3, Tubulin alpha-1A chain, Alpha-tubulin 3, Tubulin B-alpha-1, Tubulin alpha-3 chain

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제품이 사용된 논문 (5)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal for immunotherapy of cancer 13: PubMed39824529

2025

Pancreatic cancer cell-intrinsic transglutaminase-2 promotes T cell suppression through microtubule-dependent secretion of immunosuppressive cytokines.

Applications

Unspecified application

Species

Unspecified reactive species

Anton Lahusen,Nora Minhöfer,Kim-André Lohse,Christine Blechner,Jessica Lindenmayer,Tim Eiseler,Anton Wellstein,Alexander Kleger,Thomas Seufferlein,Sabine Windhorst,Yuan-Na Lin

iScience 27:111104 PubMed39473976

2024

Alternatively spliced MAP4 isoforms have key roles in maintaining microtubule organization and skeletal muscle function.

Applications

Unspecified application

Species

Unspecified reactive species

Lathan Lucas,Larissa Nitschke,Brandon Nguyen,James A Loehr,George G Rodney,Thomas A Cooper

Toxics 12: PubMed38922090

2024

Chronic Lead Exposure in Adult Mice: Associations with miR-671/CDR1as Regulation, NF-κB Signaling, and Alzheimer's Disease-like Pathology.

Applications

Unspecified application

Species

Unspecified reactive species

Mengyun Qiao,Haitao Yang,Li Liu,Tao Yu,Haihua Wang,Xiao Chen,Yi Zhang,Airu Duan,Shujun Lyu,Siyu Wu,Jingwei Xiao,Bin Li

Acta biochimica et biophysica Sinica 56:1802-1812 PubMed38818580

2024

Inducible ablation alleviates cardiac fibrosis via regulation of microtubule stability.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Wang,Xiangchong Wang,Yiyi Zhang,Yuan Mi,Yanxue Han,Yaxin Zhi,Ran Zhao,Nanqi Cui,Qianli Ma,Huaxing Zhang,Dazhong Xue,Ruoyang Qiao,Jiabing Han,Yulou Yu,Jiaxuan Li,Mohammed Shaiea,Demin Liu,Guoqiang Gu,Chuan Wang

Molecular cancer therapeutics 5:2824-33 PubMed17121929

2006

Bcl-2 down-regulation and tubulin subtype composition are involved in resistance of ovarian cancer cells to vinflunine.

Applications

Unspecified application

Species

Unspecified reactive species

Marie-Anne Estève,Manon Carré,Véronique Bourgarel-Rey,Anna Kruczynski,Giuseppina Raspaglio,Cristiano Ferlini,Diane Braguer

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Immunocytochemistry/ Immunofluorescence - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Immunohistochemistry (Frozen sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

Western blot - Anti-Detyrosinated alpha Tubulin antibody [AA12] - Loading Control (AB254154)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Reagents, Controls & Accessories

Reagents, Controls & Accessories

Sample Prep & Detection Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (5)

Product promise