Anti-DGAT2 antibody [EPR27385-45]

• WB

Supplier Data

Western blot - Anti-DGAT2 antibody [EPR27385-45] (AB325121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-3 : 180 seconds; Lanes 4-5 : 70 seconds.

All lanes:

Western blot - Anti-DGAT2 antibody [EPR27385-45] (ab325121) at 1/1000 dilution

Lane 1:

HepG2 (human hepatocellular carcinoma epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HepG2 transfected with siRNA specifically targeting DGAT2 whole cell lysate at 20 µg

Lane 3:

PAZ6 (human adipose epithelial cell) whole cell lysate at 20 µg

Lane 4:

Untreated 3T3-L1 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 5:

3T3-L1 differentiated for 6 days whole cell lysate at 20 µg

Secondary

All lanes:

Western blot at 1/20000 dilution

Observed band size: 37 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-DGAT2 antibody [EPR27385-45] (AB325121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, lung (PMID : 11481335).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-3 : 48 seconds; Lane 4 : 180 seconds.

All lanes:

Western blot - Anti-DGAT2 antibody [EPR27385-45] (ab325121) at 1/1000 dilution

Lane 1:

Human liver tissue lysate at 20 µg

Lane 2:

Human lung tissue lysate at 20 µg

Lane 3:

Human spleen tissue lysate at 20 µg

Lane 4:

Human fat tissue lysate at 20 µg

Secondary

All lanes:

Western blot at 1/2000 dilution

Observed band size: 37 kDa

false

• WB

Supplier Data

Western blot - Anti-DGAT2 antibody [EPR27385-45] (AB325121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, lung (PMID : 11481335).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 92 seconds; Lanes 2-5 : 26 seconds.

All lanes:

Western blot - Anti-DGAT2 antibody [EPR27385-45] (ab325121) at 1/1000 dilution

Lane 1:

Mouse skin tissue lysate at 20 µg

Lane 2:

Mouse brown fat tissue lysate at 20 µg

Lane 3:

Mouse liver tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

Mouse lung tissue lysate at 20 µg

Lane 6:

Mouse white fat tissue lysate at 20 µg

Secondary

All lanes:

Western blot at 1/20000 dilution

Observed band size: 37 kDa,36 kDa

false

• WB

Supplier Data

Western blot - Anti-DGAT2 antibody [EPR27385-45] (AB325121)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : spleen, lung (PMID : 11481335).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lane 1 : 125 seconds; Lanes 2-5 : 59 seconds.

All lanes:

Western blot - Anti-DGAT2 antibody [EPR27385-45] (ab325121) at 1/1000 dilution

Lane 1:

Rat skin tissue lysate at 20 µg

Lane 2:

Rat white fat tissue lysate at 20 µg

Lane 3:

Rat liver tissue lysate at 20 µg

Lane 4:

Rat spleen tissue lysate at 20 µg

Lane 5:

Rat lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot at 1/20000 dilution

Observed band size: 37 kDa,36 kDa

false