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AB235366

Anti-DHX35 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal DHX35 antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DHX35 aa 1-300.

대체 명칭 보기

C20orf15, DDX35, DHX35, Probable ATP-dependent RNA helicase DHX35, DEAH box protein 35

2 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX35 antibody (AB235366)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX35 antibody (AB235366)

Paraffin-embedded human brain tissue stained for DHX35 using ab235366 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX35 antibody (AB235366)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DHX35 antibody (AB235366)

Paraffin-embedded human testis tissue stained for DHX35 using ab235366 at 1/100 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human DHX35 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q9H5Z1

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대체품

Primary Antibodies

AB222244

Anti-DHX35 antibody

Immunocytochemistry/ Immunofluorescence - Anti-DHX35 antibody (AB222244)

  • 0
  • 0
Primary Antibodies

AB179442

Anti-DHX35 antibody [EPR12383] - C-terminal

RabMAb|Recombinant

Western blot - Anti-DHX35 antibody [EPR12383] - C-terminal (AB179442)

  • 0
  • 0

보완 제품

Primary Antibodies

AB420

Anti-Digoxigenin antibody [21H8]

  • 0
  • 0
Primary Antibodies

AB130413

Anti-GPATCH1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPATCH1 antibody (AB130413)

  • 0
  • 0
Primary Antibodies

AB115777

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

20ul selling size|RabMAb|Lab Essentials|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (AB115777)

  • 4
  • 1
Primary Antibodies

AB10805

Anti-5-methylcytosine (5-mC) antibody [33D3]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5-methylcytosine (5-mC) antibody [33D3] (AB10805)

  • 5
  • 12
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/200", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Orangutan": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein G
Purification 관련 사항
Purity >95%.
보관 버퍼
pH: 7.4 Preservative: 0.03% Proclin 300 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DHX35 also known as DExH-box Helicase 35 is a member of the DEAD box protein family characterized by its Asp-Glu-Ala-Asp (DEAD) motif. This protein has a molecular mass of approximately 82 kDa. DHX35 functions mechanically as an ATP-dependent RNA helicase unfurling RNA secondary structures during processes such as transcription and translation. It is widely expressed in various tissues but notable expression occurs in the liver and testis. These helicases are important for manipulating RNA morphology to facilitate cellular processes.
Biological function summary

DHX35 has roles in RNA metabolism influencing processes such as RNA splicing and ribosome biogenesis. It doesn't operate alone; instead it integrates into intricate complexes with other proteins to regulate RNA dynamics. Its activity is important for proper RNA association with ribonucleoprotein complexes impacting RNA-mediated gene expression regulation and maintenance of cellular homeostasis.

Pathways

DHX35 contributes significantly to the RNA processing pathways particularly the RNA splicing pathway. Its interactions with proteins such as small nuclear ribonucleoproteins (snRNPs) highlight its participation in the spliceosome complex. Furthermore DHX35 also plays a part in ribosome biogenesis pathways linking to proteins such as nucleolin which assist in rRNA transcription and ribosomal assembly.

DHX35 shows connections to genetic disorders like Male Infertility where dysregulation impacts sperm development and function. Additionally variations in DHX35 expression or function have associations with hepatocellular carcinoma suggesting a role in tumor biology. These associations stem from interactions with other proteins like DDX3X which also influence RNA processing in cancer and reproductive health.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

May be involved in pre-mRNA splicing.
See full target information DHX35
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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

PLoS pathogens 20:e1012379 PubMed39037956

2024

The RNA helicase DHX35 functions as a co-sensor for RIG-I-mediated innate immunity.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Qiao,Shan Zhu,Ning Yang,Shan-Shan Zou,Bao Gao,Jing Wu,Chunyan Liu,Xiaoping Li,Yong-Jun Liu,Jingtao Chen

Journal of clinical medicine 10: PubMed33925488

2021

Combined Inhibition of TGF-β1-Induced EMT and PD-L1 Silencing Re-Sensitizes Hepatocellular Carcinoma to Sorafenib Treatment.

Applications

Unspecified application

Species

Unspecified reactive species

Ritu Shrestha,Prashanth Prithviraj,Kim R Bridle,Darrell H G Crawford,Aparna Jayachandran
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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