AB245659

Anti-DIAPH3 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal DIAPH3 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human DIAPH3 aa 1-50.

대체 명칭 보기

DIAP3, DIAPH3, Protein diaphanous homolog 3, Diaphanous-related formin-3, MDia2, DRF3

2 이미지

Supplier Data

Immunoprecipitation - Anti-DIAPH3 antibody (AB245659)

DIAPH3 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

ab245659 used for IP at 6 μg per reaction. For WB 1 μg/ml.

Lane 1 : ab245659 IP in HeLa whole cell lysate.
Lane 2 : Control IgG in HeLa whole cell lysate.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-DIAPH3 antibody (ab245659)

Predicted band size: 137 kDa

false

Supplier Data

Western blot - Anti-DIAPH3 antibody (AB245659)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-DIAPH3 antibody (ab245659) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 137 kDa

false

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human DIAPH3 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9NSV4

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab245659 was affinity purified using an epitope specific to DIAPH3 immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DIAPH3 also known as diaphanous-related formin 3 is a protein with an approximate mass of 130 kDa. It plays an important role in the regulation of the actin cytoskeleton essential for the dynamic reorganization of actin filaments. DIAPH3 primarily functions by mediating actin nucleation and elongation facilitating cellular processes such as shape changes adhesion and division. The protein is expressed in various tissues with notable expression in the brain testis and pancreas.

Biological function summary

DIAPH3 is a part of the formin family and acts by promoting the polymerization of actin filaments. It forms a complex with profilin and other actin-binding proteins maintaining the balance of cellular architecture. DIAPH3 contributes to the maintenance of normal cellular structure and dynamics participating in cell migration and morphogenesis. Its interaction with GTPases like RhoA regulates the initiation and stabilization of actin filaments directly impacting cell mechanics.

Pathways

DIAPH3 engages in the Rho GTPase signaling pathway impacting actin dynamics and playing roles in cytoskeletal remodelling. It also participates in the mTOR signaling pathway influencing cell growth and metabolism. Within these pathways DIAPH3 works closely with proteins such as mTOR contributing to cellular responses like growth proliferation and survival. These pathways highlight DIAPH3’s involvement in coordinating cellular structural integrity and function.

Research has implicated DIAPH3 in sensory hearing loss and cancer progression. In hearing loss mutations in DIAPH3 disrupt the organization of actin filaments critical for auditory function. In the context of cancer abnormal DIAPH3 expression relates to increased cellular motility and invasion facilitating metastasis. In these conditions DIAPH3 interacts with proteins such as E-cadherin affecting cellular adhesion and communication and emphasizing its significance in disease mechanisms.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Actin nucleation and elongation factor required for the assembly of F-actin structures, such as actin cables and stress fibers. Required for cytokinesis, stress fiber formation and transcriptional activation of the serum response factor. Binds to GTP-bound form of Rho and to profilin : acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Also acts as an actin nucleation and elongation factor in the nucleus by promoting nuclear actin polymerization inside the nucleus to drive serum-dependent SRF-MRTFA activity.

See full target information DIAPH3

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Endokrynologia Polska 75:501-509 PubMed39376176

2024

The functional DIAPH3-FOXM1 interaction modulates the aggressive transformation of anaplastic thyroid carcinoma cells and Wnt/β-catenin signalling.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Shi,Hao Fang,Kai Fu,Zhen Zhao,Fei Yang,Yan Liu

Molecular medicine reports 25: PubMed34878155

2021

IRF2 contributes to myocardial infarction via regulation of GSDMD induced pyroptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Yongxing Li,Yan Wang,Hua Guo,Qinghua Wu,Yamin Hu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DIAPH3 antibody

Immunoprecipitation - Anti-DIAPH3 antibody (AB245659)

Western blot - Anti-DIAPH3 antibody (AB245659)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise