AB223767

Anti-DIS3 antibody

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(2 제품이 사용된 논문 )

Rabbit Polyclonal DIS3 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human DIS3 aa 800-950.

대체 명칭 보기

KIAA1008, RRP44, DIS3, Exosome complex exonuclease RRP44, Protein DIS3 homolog, Ribosomal RNA-processing protein 44

7 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3 antibody (AB223767)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3 antibody (AB223767)

Paraffin-embedded human cervix tissue stained for DIS3 using ab223767 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3 antibody (AB223767)

Paraffin-embedded human testis tissue stained for DIS3 using ab223767 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3 antibody (AB223767)

Paraffin-embedded human cerebellum tissue stained for DIS3 using ab223767 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-DIS3 antibody (AB223767)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-DIS3 antibody (AB223767)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for DIS3 (green) using ab223767 at 4 μg/ml in ICC.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3 antibody (AB223767)

Paraffin-embedded human lymph node tissue stained for DIS3 using ab223767 at 1/200 dilution in immunohistochemical analysis.

Supplier Data

Western blot - Anti-DIS3 antibody (AB223767)

All lanes:

Western blot - Anti-DIS3 antibody (ab223767) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 109 kDa

false

Supplier Data

Western blot - Anti-DIS3 antibody (AB223767)

All lanes:

Western blot - Anti-DIS3 antibody (ab223767) at 1/100 dilution

All lanes:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate

Predicted band size: 109 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human DIS3 aa 800-950. The exact immunogen used to generate this antibody is proprietary information.

Q9Y2L1

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DIS3 also known as RRP44 is an exoribonuclease and an essential component of the RNA degradation machinery. It possesses a molecular mass of approximately 110 kDa and functions as an RNA helicase and 3'-5' exoribonuclease. DIS3 is expressed in various tissues including the bone marrow and lymphoid tissues. The protein acts to degrade defective RNA molecules and regulate RNA levels ensuring accurate gene expression and proper cellular function.

Biological function summary

DIS3 participates in the RNA exosome complex a multi-protein complex responsible for processing and degrading RNA. This complex processes a wide range of RNA substrates including pre-rRNA snRNA and snoRNA. DIS3 performs its function by catalyzing the exonucleolytic cleavage of these RNA molecules regulating their abundance and contributing to RNA quality control. As a critical member of the exosome DIS3 is essential for cellular RNA homeostasis and plays a role in maintaining the stability of the transcriptome.

Pathways

RNA metabolism and degradation significantly involve DIS3 impacting mRNA surveillance and decay pathways. It notably contributes to the Nonsense-Mediated mRNA Decay (NMD) pathway which detects and degrades mRNAs containing premature stop codons. DIS3 interacts with other exosome components such as RRP6 and MTR4 facilitating the processing of diverse RNA species. The protein’s role is integral to overall RNA turnover and quality control ensuring effective removal of defective RNA transcripts and preventing inappropriate protein synthesis.

Mutations or dysregulation of DIS3 relate to multiple myeloma and acute myeloid leukemia. In these conditions aberrant RNA degradation may contribute to the pathogenesis by altering normal RNA processing and expression profiles. DIS3 mutations can lead to impaired function of the exosome enhancing the survival and proliferation of malignant cells. Additionally proteins such as RRP6 are connected with DIS3 through their roles in the exosome machinery suggesting combined involvement in RNA processing-related diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as antisense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.

See full target information DIS3

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Scientific reports 15:32507 PubMed40940393

2025

Age, muscle, and gender specific characterization of muscle degeneration in a mouse model of calpainopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolina Südkamp,Jacqueline Heinen-Weiler,Marlena Rohm,Michaela Zaik,Nassam Daya,Anne-Katrin Güttsches,Carsten Theiss,Andreas Roos,Tobias Ruck,Frank Jacobsen,Lara Schlaffke,Matthias Vorgerd

Cell reports 42:112047 PubMed36724075

2023

RNA exosome ribonuclease DIS3 degrades Pou6f1 to promote mouse pre-implantation cell differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Di Wu,Jurrien Dean

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DIS3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3 antibody (AB223767)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3 antibody (AB223767)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3 antibody (AB223767)

Immunocytochemistry/ Immunofluorescence - Anti-DIS3 antibody (AB223767)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DIS3 antibody (AB223767)

Western blot - Anti-DIS3 antibody (AB223767)

Western blot - Anti-DIS3 antibody (AB223767)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (2)

Product promise