Anti-Dishevelled 2 antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 2 antibody (AB228804)

HCT 116 (human colorectal carcinoma cell line) cells stained for Dishevelled 2 (green) using ab228804 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

Blue : Hoechst 33342 staining.

• WB

Supplier Data

Western blot - Anti-Dishevelled 2 antibody (AB228804)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-Dishevelled 2 antibody (ab228804) at 1/5000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell extract at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract at 30 µg

Lane 4:

HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract at 30 µg

Predicted band size: 78 kDa

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• WB

Lab

Western blot - Anti-Dishevelled 2 antibody (AB228804)

False colour image of Western blot : Anti-Dishevelled 2 antibody staining at 1/5000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab228804 was shown to bind specifically to Dishevelled 2. A band was observed at 85-90 kDa in wild-type A549 cell lysates with no signal observed at this size in dvl2 knockout cell line ab277903 (knockout cell lysate ab288317). To generate this image, wild-type and dvl2 knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-Dishevelled 2 antibody (ab228804) at 1/5000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

dvl2 knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human DVL2 knockout A549 cell line (<a href='/ko/products/cell-lines/human-dvl2-knockout-a549-cell-line-ab277903'>ab277903</a>)

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

THP-1 cell lysate at 20 µg

Predicted band size: 78 kDa

Observed band size: 85 kDa,85-90 kDa

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