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AB325945

Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free

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Rabbit Recombinant Monoclonal Dishevelled 2 antibody. Carrier free. Suitable for Dot, IHC-P, WB and reacts with Transfected cell lysate - Human, Human, Mouse samples.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)

This data was developed using ab325939, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human ovary tissue labeling Dishevelled 2 with ab325939 at 1/1000 (0.509 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).

Positive staining on human ovary. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND®RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (BOND® Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)

This data was developed using ab325939, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human cerebrum tissue labeling Dishevelled 2 with ab325939 at 1/1000 (0.509 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).

Low expression tissue : no staining on human cerebrum (PMID : 21990322). The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND®RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)

This data was developed using ab325939, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human ovarian cancer tissue labeling Dishevelled 2 with ab325939 at 1/1000 (0.509 µg/ml) dilution followed by a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).

Positive staining on human ovarian cancer. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)

This data was developed using ab325939, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Dishevelled 2 with ab325939 at 1/1000 (0.509 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Low expression tissue : no staining on human liver. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)

This data was developed using ab325939, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) Wild-type A549 (human lung carcinoma epithelial cell) cell pellet and (B) DVL2 knockout A549 cell pellet labeling Dishevelled 2 with ab325939 at 1/2000 (0.255 µg/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).

Positive staining on (A) Wild-type A549 cell pellet, no staining on (B) DVL2 knockout A549 cell pellet. The primary antibody was incubated for 30 mins at room temperature.

The immunostaining was performed on a Leica Biosystems BOND®RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)
  • WB

Lab

Western blot - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)

This data was developed using ab325939, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Performed under reducing conditions.

In Western blot ab325939 was shown to bind specifically to Dishevelled 2. Target of interest was observed at 95 kDa in wild-type A549 cell lysates (lane 3) with no signal observed at this size in Dishevelled 2 knockout cell line (lane 4) (knockout cell line ab277903 / knockout cell lysate ab288317).

Low expression : liver lung.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lanes 1-4 : 26 seconds; Lane 5 : 6 seconds; Lane 6 : 26 seconds.

All lanes:

Western blot - Anti-Dishevelled 2 antibody [EPR30298-74] (<a href='/ko/products/primary-antibodies/dishevelled-2-antibody-epr30298-74-ab325939'>ab325939</a>) at 1/1000 dilution

Lane 1:

Human ovary tissue lysate at 20 µg

Lane 2:

Human testis tissue lysate at 20 µg

Lane 3:

Human liver tissue lysate at 20 µg

Lane 4:

Human lung tissue lysate at 20 µg

Lane 5:

A549 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 6:

Dishevelled 2 knockout A549 whole cell lysate at 20 µg

Lane 7:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 8:

NCI-H1299 (human lung carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 95 kDa,36 kDa

false

Western blot - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)
  • WB

Lab

Western blot - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)

This data was developed using ab325939, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In lanes 1 and 4, the lysates were stored at -80degC prior to Western Blotting. The bands beneath the target band (95 kDa) are likely to be degradation products. In lanes 2-3, to minimize protein degradation, cells were lysed immediately after harvest and then applied to a gel and transfer membrane for Western blotting as soon as possible.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Dishevelled 2 antibody [EPR30298-74] (<a href='/ko/products/primary-antibodies/dishevelled-2-antibody-epr30298-74-ab325939'>ab325939</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) frozen whole cell lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) fresh whole cell lysate at 20 µg

Lane 3:

NIH/3T3 (mouse embryonic fibroblast) fresh whole cell lysate at 20 µg

Lane 4:

NIH/3T3 (mouse embryonic fibroblast) frozen whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 95 kDa,36 kDa

false

Exposure time: 59s

Western blot - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)
  • WB

Lab

Western blot - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)

This data was developed using ab325939, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : brain, liver, spleen.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lanes 1-2 : 180 seconds; Lanes 3-5 : 6 seconds.

All lanes:

Western blot - Anti-Dishevelled 2 antibody [EPR30298-74] (<a href='/ko/products/primary-antibodies/dishevelled-2-antibody-epr30298-74-ab325939'>ab325939</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse liver tissue lysate at 20 µg

Lane 3:

Mouse testis tissue lysate at 20 µg

Lane 4:

Mouse spleen tissue lysate at 20 µg

Lane 5:

L Cells (mouse Subcutaneous connective tissue fibroblast) whole cell lysate at 20 µg

Lane 6:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 7:

CT26.WT (mouse colon fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 95 kDa,36 kDa

false

Western blot - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)
  • WB

Lab

Western blot - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)

This data was developed using ab325939, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Dishevelled 2 antibody [EPR30298-74] (<a href='/ko/products/primary-antibodies/dishevelled-2-antibody-epr30298-74-ab325939'>ab325939</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HEK-293 transfected with siRNA specifically targeting Dishevelled 2 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 95 kDa,36 kDa

false

Exposure time: 59s

Dot Blot - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)
  • Dot

Lab

Dot Blot - Anti-Dishevelled 2 antibody [EPR30298-74] - BSA and Azide free (AB325945)

This data was developed using ab325939, the same antibody clone in a different buffer formulation.

Dot blot analysis of Dishevelled 2 using ab325939 at 1 : 1000 (0.509 µg/ml) followed by a Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) at 1 : 100000 dilution.

Lane 1 : 293T (human embryonic kidney epithelial cell) cells transfected with human Dishevelled 1 expression vector containing a myc-His-tag® whole cell lysate

Lane 2 : 293T cells transfected with human Dishevelled 2 expression vector containing a myc-His-tag® whole cell lysate

Lane 3 : 293T cells transfected with human Dishevelled 3 expression vector containing a myc-His-tag® whole cell lysate

Exposure time : 3 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human Dishevelled 1 or Dishevelled 3 by dot blot.

All lanes:

Dot Blot - Anti-Dishevelled 2 antibody [EPR30298-74] (<a href='/ko/products/primary-antibodies/dishevelled-2-antibody-epr30298-74-ab325939'>ab325939</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with human Dishevelled 1 expression vector containing a myc-His-tag® whole cell lysate

Lane 2:

293T cells transfected with human Dishevelled 2 expression vector containing a myc-His-tag® whole cell lysate

Lane 3:

293T cells transfected with human Dishevelled 3 expression vector containing a myc-His-tag® whole cell lysate

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false

Exposure time: 3s

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR30298-74

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

IHC-P, WB, Dot

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>Unsuitable for IHC-Fr in fresh frozen tissue. This antibody does not cross-react with overexpressed human Dishevelled 1 or Dishevelled 3 by dot blot.</p>

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보완 제품

Primary Antibodies

AB23683

Anti-FOXO3A antibody

BOND RX™ Validated

Immunocytochemistry/ Immunofluorescence - Anti-FOXO3A antibody (AB23683)

  • 0
  • 2
Primary Antibodies

AB40772

Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Western blot - Anti-E Cadherin antibody [EP700Y] - Intercellular Junction Marker (AB40772)

  • 4
  • 23
Primary Antibodies

AB124933

Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145]

RabMAb|Recombinant

Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (AB124933)

  • 0
  • 0
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

20ul selling size|KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

  • 5
  • 15
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "Dot" : {"fullname" : "Dot Blot", "shortname":"Dot"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Mouse": { "Dot-species-checked": "guaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "Dot-species-checked": "testedAndGuaranteed", "Dot-species-dilution-info": "", "Dot-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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제품 세부 정보

ab325945 is the carrier-free version of ab325939

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Plays a role in the signal transduction pathways mediated by multiple Wnt genes (PubMed : 24616100). Participates both in canonical and non-canonical Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling.
See full target information DVL2

대체 명칭 보기

Segment polarity protein dishevelled homolog DVL-2, Dishevelled-2, DSH homolog 2, DVL2, Segment polarity protein dishevelled homolog DVL-2, Dishevelled-2, DSH homolog 2
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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