Anti-Dityrosine antibody [10A6]

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-Dityrosine antibody [10A6] (AB243067)

Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells (+/- 250 μM hydrogen peroxide treatment for 24 hours) labeling Dityrosine with ab243067 at 1/50 dilution for 30 minutes on ice compared with a Isotype control IgG and an unlabeled control. A Goat Anti-Mouse : PE at 1/100 dilution was used as the secondary antibody.

• WB

Supplier Data

Western blot - Anti-Dityrosine antibody [10A6] (AB243067)

Primary incubation for 2 hours at room temperature.

Secondary incubation for 1 hour at room temperature.

Block : 5% Skimmed milk in TBST.

All lanes:

Western blot - Anti-Dityrosine antibody [10A6] (ab243067) at 1/1000 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 12 µg

Lane 2:

HeLa (treated with hydrogen peroxide) cell lysate at 12 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1/2000 dilution

true

Exposure time: 5min

• WB

Ap

Western blot - Anti-Dityrosine antibody [10A6] (AB243067)

Primary incubation for 2 hours at room temperature.

Secondary incubation for 1 hour at room temperature.

Block : 5% Skimmed milk in TBST.

All lanes:

Western blot - Anti-Dityrosine antibody [10A6] (ab243067) at 1/1000 dilution

Lane 1:

BSA at 1 µg

Lane 2:

3,5-Dibromotyrosine-BSA at 1 µg

Lane 3:

Dityrosine-BSA at 1 µg

Lane 4:

Bromotyrosine-BSA at 1 µg

Lane 5:

7-ketocholesterol-BSA at 1 µg

Secondary

All lanes:

Synthetic Dityrosine conjugated to Keyhole Limpet Kemocyanin (KLH) at 1/20000 dilution

true

Exposure time: 5min