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AB242464

Anti-DKK1 antibody [EPR17011-167] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal DKK1 antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.

대체 명칭 보기

Dickkopf-related protein 1, Dickkopf-1, Dkk-1, mDkk-1, Dkk1

1 이미지
Sandwich ELISA - Anti-DKK1 antibody [EPR17011-167] - BSA and Azide free (Capture) (AB242464)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-DKK1 antibody [EPR17011-167] - BSA and Azide free (Capture) (AB242464)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab197746).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17011-167

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB100501

Human DKK1 ELISA Kit (Dickkopf-1)

Sandwich ELISA - Human DKK1 ELISA Kit (Dickkopf-1) (AB100501)

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Primary Antibodies

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Primary Antibodies

AB24982

Cy5 ® Anti-CD45.1 antibody [A20]

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Primary Antibodies

AB252149

Alexa Fluor® 488 Anti-CD4 antibody [EPR20122]

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Flow Cytometry - Alexa Fluor® 488 Anti-CD4 antibody [EPR20122] (AB252149)

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Primary Antibodies

AB115777

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

20ul selling size|RabMAb|Lab Essentials|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (AB115777)

  • 4
  • 1
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab242464 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse DKK1. The recommended pair for sandwich ELISA is:
Capture: ab242464, Mouse DKK1 Capture Antibody (unconjugated)
Detector: ab242715, Mouse DKK1 Detector Antibody (unconjugated)
The reference range value is 20 - 1250 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The DKK1 protein also known as Dickkopf-1 is a secreted protein with a mass of approximately 28-30 kDa. It plays a mechanical role in inhibiting the Wnt signaling pathway by binding to LRP5/6 co-receptors preventing Wnt ligands from activating the pathway. DKK1 is expressed in various tissues including the bone brain and placenta reflecting its involvement in several biological processes. Its capacity to bind and neutralize the Wnt pathway components cements DKK1's role as a significant modulator of cellular functions.
Biological function summary

The inhibition of the Wnt signaling by DKK1 alters cell proliferation differentiation and migration. DKK1 does not function as part of a large multi-protein complex but interacts directly with specific co-receptors and modulates the activity of key signaling molecules. This regulatory mechanism controls processes like bone formation and neurogenesis indicating DKK1's broad impact in development and tissue homeostasis. Its expression pattern and biological role position it as an important player in maintaining proper cellular signaling environments.

Pathways

DKK1 is a critical player in the Wnt signaling and bone remodeling pathways. Through its interaction with Wnt pathway components like LRP5/6 DKK1 modulates these pathways affecting downstream proteins such as β-catenin and influencing gene transcription. In the bone remodeling context DKK1's ability to regulate osteoblast activity connects it to other related proteins like sclerostin contributing to bone density and health.

Elevated levels of DKK1 are observed in conditions like osteoporosis and cancer. In osteoporosis DKK1's inhibition of Wnt signaling leads to reduced bone formation implicating it alongside proteins like sclerostin in deteriorating bone health. In cancer DKK1's dysregulation can promote tumorigenesis by altering cell signaling and microenvironment interactions. These roles underline the potential of targeting DKK1 for therapeutic purposes in treating these diseases.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed : 18524778). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti-apoptotic activity (PubMed : 26206087). Plays a role in limb development; attenuates Wnt signaling in the developing limb to allow normal limb patterning (PubMed : 18505822).
See full target information Dkk1
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Product promise

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