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AB325661

Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric)

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Chicken Recombinant Monoclonal PSD95 antibody. Suitable for IHC-P, WB and reacts with Rat samples.
3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric) (AB325661)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric) (AB325661)

Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of DLG4. The sections were stained with ab325661 at 1 : 500 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody specifically stains the excitatory synapses in the granular layer of the cerebellum. Magnification 400X.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric) (AB325661)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric) (AB325661)

Formalin fixed, paraffin-embedded rat brain cerebellum region showing staining of DLG4. The sections were stained with ab325661 at 1 : 500 dilution (green). The stained sections were mounted with Fluoroshield with DAPI and DABCO mounting medium. DAPI nuclear stain (blue) shows cell nuclei. The antibody specifically stains the excitatory synapses in the granular layer of the cerebellum. Magnification 100X.

Western blot - Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric) (AB325661)
  • WB

Supplier Data

Western blot - Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric) (AB325661)

Western blotting of rat brain homogenate with an anti-PSD-95 mouse Mab and ab325661 antibodies (2 µg/ml). The mouse Mab and chicken Mab were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. ab325661 recognizes endogenous DLG4 with similar sensitivity as the anti-PSD-95 mouse Mab in the rat brain homogenate at 95-110 kDa. PSD-95 is an alternative name for DLG4.

All lanes:

Western blot - Anti-DLG4 antibody [K28/43] - Chicken IgY (Chimeric) (ab325661) at 2 µg/mL

All lanes:

rat brain homogenate

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 95-110 kDa

false

주요 정보

Host species

Chicken

Clonality

Monoclonal

Clone number

K28/43

Isotype

IgY

Carrier free

No

Reacts with

Rat

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

<p>No cross-reactivity against recombinant Chapsyn-110, SAP97 or SAP102&#46;</p>

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보완 제품

Primary Antibodies

AB254349

Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker

BOND RX™ Validated|Lab Essentials|20ul selling size|RabMAb|Advanced Validation|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Synapsin I antibody [EPR23531-50] - Synaptic Marker (AB254349)

  • 4
  • 1
Primary Antibodies

AB32127

Anti-Synaptophysin antibody [YE269] - Synaptic Marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Western blot - Anti-Synaptophysin antibody [YE269] - Synaptic Marker (AB32127)

  • 5
  • 39
Primary Antibodies

AB178846

Anti-Iba1 antibody [EPR16588] - Microglia marker

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Iba1 antibody [EPR16588] - Microglia marker (AB178846)

  • 5
  • 50
Primary Antibodies

AB177487

Anti-NeuN antibody [EPR12763] - Neuronal Marker

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Frozen sections) - Anti-NeuN antibody [EPR12763] - Neuronal Marker (AB177487)

  • 5
  • 76
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2 µg/mL", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
Purification 관련 사항
This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone K28/43 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone K28/43 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.
보관 버퍼
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Postsynaptic scaffolding protein that plays a critical role in synaptogenesis and synaptic plasticity by providing a platform for the postsynaptic clustering of crucial synaptic proteins (PubMed : 15317815, PubMed : 15358863, PubMed : 19596852, PubMed : 20628354, PubMed : 23300088, PubMed : 26679993). Interacts with the cytoplasmic tail of NMDA receptor subunits and shaker-type potassium channels. Required for synaptic plasticity associated with NMDA receptor signaling. Overexpression or depletion of DLG4 changes the ratio of excitatory to inhibitory synapses in hippocampal neurons. May reduce the amplitude of ASIC3 acid-evoked currents by retaining the channel intracellularly. May regulate the intracellular trafficking of ADR1B (By similarity). Also regulates AMPA-type glutamate receptor (AMPAR) immobilization at postsynaptic density keeping the channels in an activated state in the presence of glutamate and preventing synaptic depression (PubMed : 19596852). Under basal conditions, cooperates with FYN to stabilize palmitoyltransferase ZDHHC5 at the synaptic membrane through FYN-mediated phosphorylation of ZDHHC5 and its subsequent inhibition of association with endocytic proteins (By similarity).
See full target information Dlg4

대체 명칭 보기

Dlgh4, Psd95, Dlg4, Disks large homolog 4, Postsynaptic density protein 95, Synapse-associated protein 90, PSD-95, SAP-90, SAP90
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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