Anti-DLK (MAP3K12) antibody [EPR30420-541]

• WB

Lab

Western blot - Anti-DLK (MAP3K12) antibody [EPR30420-541] (AB325176)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen, lung(PMID : 8769565; PMID : 7983011)

Low expression : HepG2, A431

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 35101451).

The identity of the bands below 75 kDa are unknown.

Lanes 1-3 are incubated with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/20000 and lanes 4-7 are incubated with Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

Exposure time : Lanes 1-3 : 70 seconds; Lanes 4-7 : 103 seconds

All lanes:

Western blot - Anti-DLK (MAP3K12) antibody [EPR30420-541] (ab325176) at 1/1000 dilution

Lane 1:

SK-N-BE(2) (human neuroblastoma neuroblast) whole lysate at 20 µg

Lane 2:

HepG2 (human hepatocellular carcinoma epithelial cell) whole lysate at 20 µg

Lane 3:

A431 (human epidermoid carcinoma epithelial cell) whole lysate at 20 µg

Lane 4:

Human hippocampus tissue lysate at 20 µg

Lane 5:

Human hypothalamus tissues lydate at 20 µg

Lane 6:

human spleen tissue lysate at 20 µg

Lane 7:

Human lung tissue lysate at 20 µg

Secondary

Lanes 1 - 3:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Lanes 4 - 7:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 120 kDa,36 kDa

false

• WB

Lab

Western blot - Anti-DLK (MAP3K12) antibody [EPR30420-541] (AB325176)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : spleen, heart, liver(PMID : 8769565; PMID : 7983011)

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 35101451).

The identity of the bands below 75 kDa are unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-DLK (MAP3K12) antibody [EPR30420-541] (ab325176) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) at 20 µg

Lane 2:

Mouse hypothalamus tissue lysate at 20 µg

Lane 3:

Mouse cerebellum tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse liver tissue lysate at 20 µg

Lane 6:

Mouse heart tissue lysate at 20 µg

Lane 7:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 120 kDa,36 kDa

false

Exposure time: 37s

• I-ELISA

Lab

Indirect ELISA - Anti-DLK (MAP3K12) antibody [EPR30420-541] (AB325176)

Indirect ELISA analysis of ab325176 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : DLK (MAP3K12);LZK (MAP3K13).

Antigen concentration : 1000 ng/ml