AB199534

Anti-DMGDH antibody [EPR16907]

RabMAb
Recombinant
각 태그의 뜻

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Rabbit Recombinant Monoclonal DMGDH antibody. Suitable for WB and reacts with Mouse, Rat, Human samples. Cited in 1 publication.

대체 명칭 보기

ME2GLYDH, DMGDH

3 이미지

Supplier Data

Western blot - Anti-DMGDH antibody [EPR16907] (AB199534)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DMGDH antibody [EPR16907] (ab199534) at 1/1000 dilution

All lanes:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 97 kDa

Observed band size: 97 kDa

false

Exposure time: 1min

Supplier Data

Western blot - Anti-DMGDH antibody [EPR16907] (AB199534)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DMGDH antibody [EPR16907] (ab199534) at 1/1000 dilution

All lanes:

Human fetal liver tissue lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

Predicted band size: 34 kDa,36 kDa,97 kDa

Observed band size: 97 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-DMGDH antibody [EPR16907] (AB199534)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-DMGDH antibody [EPR16907] (ab199534) at 1/1000 dilution

Lane 1:

Mouse kidney lysate at 10 µg

Lane 2:

Mouse spleen lysate at 10 µg

Lane 3:

Rat kidney lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

false

Exposure time: 3min

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR16907

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

DMGDH also known as dimethylglycine dehydrogenase is a mitochondrial enzyme with a mass of approximately 103 kDa. This protein is expressed predominantly in liver and kidney tissues. DMGDH plays an important role in the degradation of choline where it catalyzes the oxidative demethylation of dimethylglycine into sarcosine while producing formaldehyde and hydrogen peroxide. This process is an important step in the choline oxidation pathway which helps maintain the methylation balance in the cell.

Biological function summary

DMGDH contributes to the one-carbon metabolism pathway which involves the transfer of one-carbon units essential for various biosynthetic reactions. It does not form part of a larger enzymatic complex but operates as an independent enzyme within the mitochondrial matrix. Its activity is important for methyl cycle function influencing processes like nucleotide synthesis and amino acid metabolism. The enzyme helps connect choline metabolism to broader metabolic networks impacting cellular methyl group availability.

Pathways

DMGDH is integral to the choline metabolism pathway and the one-carbon metabolic pathway. Choline is oxidized to produce substrates for the methionine cycle affecting homocysteine levels and S-adenosylmethionine production. DMGDH's function is closely linked to other enzymes like dimethylglycine N-methyltransferase (DNMT) which also engages in methyl group transfer. Together these enzymes collaborate to maintain cellular methylation and detoxification pathways.

DMGDH dysfunction has been associated with metabolic conditions such as homocystinuria and cardiovascular disease. Impaired DMGDH activity can lead to increased levels of dimethylglycine which in turn may disrupt methylation balance and contribute to metabolic abnormalities. While DMGDH does not directly interact with any known disease-specific proteins its role in maintaining proper methylation highlights its importance in disease pathology. By influencing levels of substrates like homocysteine DMGDH indirectly connects to disorders linked to methylation dysregulation.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalyzes the demethylation of N,N-dimethylglycine to sarcosine. Also has activity with sarcosine in vitro.

See full target information Dimethylglycine dehydrogenase, mitochondrial

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of biological chemistry 299:102880 PubMed36626984

2023

Disruption of the interaction between mutationally activated Gα and Gβγ attenuates aberrant signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Jenna L Aumiller,Philip B Wedegaertner

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DMGDH antibody [EPR16907]

Western blot - Anti-DMGDH antibody [EPR16907] (AB199534)

Western blot - Anti-DMGDH antibody [EPR16907] (AB199534)

Western blot - Anti-DMGDH antibody [EPR16907] (AB199534)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise