AB31777

Anti-DNA polymerase alpha/POLA antibody

Name: Anti-DNA polymerase alpha/POLA antibody
Brand: Abcam Limited
SKU: AB31777
Rating: 4 (3 reviews)

(3 리뷰들)

(14 제품이 사용된 논문 )

Rabbit Polyclonal DNA polymerase alpha/POLA antibody. Suitable for WB, IHC-P and reacts with Human, Xenopus laevis, Chicken samples. Cited in 14 publications.

대체 명칭 보기

POLA, POLA1, DNA polymerase alpha catalytic subunit, DNA polymerase alpha catalytic subunit p180

1 이미지

Unknown

Western blot - Anti-DNA polymerase alpha/POLA antibody (AB31777)

All lanes:

Western blot - Anti-DNA polymerase alpha/POLA antibody (ab31777) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

Western blot - Jurkat whole cell lysate (<a href='/ko/products/cell-lysates/jurkat-whole-cell-lysate-ab7899'>ab7899</a>) at 20 µg

Lane 3:

Western blot - A-431 whole cell lysate (<a href='/ko/products/cell-lysates/a-431-whole-cell-lysate-ab7909'>ab7909</a>) at 20 µg

Secondary

All lanes:

Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Predicted band size: 166 kDa

Observed band size: 180 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Xenopus laevis, Chicken

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-5 µg/mL", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Catalytic subunit of the DNA polymerase alpha complex (also known as the alpha DNA polymerase-primase complex) which plays an essential role in the initiation of DNA synthesis. During the S phase of the cell cycle, the DNA polymerase alpha complex (composed of a catalytic subunit POLA1, a regulatory subunit POLA2 and two primase subunits PRIM1 and PRIM2) is recruited to DNA at the replicative forks via direct interactions with MCM10 and WDHD1. The primase subunit of the polymerase alpha complex initiates DNA synthesis by oligomerising short RNA primers on both leading and lagging strands. These primers are initially extended by the polymerase alpha catalytic subunit and subsequently transferred to polymerase delta and polymerase epsilon for processive synthesis on the lagging and leading strand, respectively. The reason this transfer occurs is because the polymerase alpha has limited processivity and lacks intrinsic 3' exonuclease activity for proofreading error, and therefore is not well suited for replicating long complexes. In the cytosol, responsible for a substantial proportion of the physiological concentration of cytosolic RNA : DNA hybrids, which are necessary to prevent spontaneous activation of type I interferon responses (PubMed : 27019227).

See full target information POLA1

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제품이 사용된 논문 (14)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 16:5706 PubMed40593507

2025

DNA polymerase α/primase extraction from chromatin by VCP/p97 restricts ATR activation during unperturbed DNA replication.

Applications

Unspecified application

Species

Unspecified reactive species

Sara Rodríguez-Acebes,Rodrigo Martín-Rufo,Alicia Gómez-Moya,Scott B Churcher,Alejandro Fernández-Llorente,Guillermo de la Vega-Barranco,Alejandra Perona,Pilar Oroz,Elena Martín-Doncel,Luis Ignacio Toledo,Juan Méndez,Emilio Lecona

International journal of molecular sciences 24: PubMed37762371

2023

The Antitumor Effect of the DNA Polymerase Alpha Inhibitor ST1926 in Glioblastoma: A Proteomics Approach.

Applications

Unspecified application

Species

Unspecified reactive species

Chirine El-Baba,Zeinab Ayache,Mona Goli,Berthe Hayar,Zeinab Kawtharani,Claudio Pisano,Firas Kobeissy,Yehia Mechref,Nadine Darwiche

Nucleic acids research 49:11653-11665 PubMed34718732

2021

CST does not evict elongating telomerase but prevents initiation by ssDNA binding.

Applications

Unspecified application

Species

Unspecified reactive species

Arthur J Zaug,Ci Ji Lim,Conner L Olson,Maria T Carilli,Karen J Goodrich,Deborah S Wuttke,Thomas R Cech

Molecular cell 80:682-698.e7 PubMed33152268

2020

Hyperactive CDK2 Activity in Basal-like Breast Cancer Imposes a Genome Integrity Liability that Can Be Exploited by Targeting DNA Polymerase ε.

Applications

Unspecified application

Species

Unspecified reactive species

Vladislav O Sviderskiy,Lili Blumenberg,Elizabeth Gorodetsky,Triantafyllia R Karakousi,Nicole Hirsh,Samantha W Alvarez,Erdem M Terzi,Efiyenia Kaparos,Gabrielle C Whiten,Shakirah Ssebyala,Peter Tonzi,Hannan Mir,Benjamin G Neel,Tony T Huang,Sylvia Adams,Kelly V Ruggles,Richard Possemato

The Journal of biological chemistry 295:15029-15044 PubMed32839273

2020

Implications of RNG140 (caprin2)-mediated translational regulation in eye lens differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Kaori Nakazawa,Yuichi Shichino,Shintaro Iwasaki,Nobuyuki Shiina

Cell reports 30:2416-2429.e7 PubMed32075739

2020

Physiological Tolerance to ssDNA Enables Strand Uncoupling during DNA Replication.

Applications

Unspecified application

Species

Unspecified reactive species

Amaia Ercilla,Jan Benada,Sampath Amitash,Gijs Zonderland,Giorgio Baldi,Kumar Somyajit,Fena Ochs,Vincenzo Costanzo,Jiri Lukas,Luis Toledo

Cell 174:803-817.e16 PubMed30057114

2018

Cross-talk between Lysine-Modifying Enzymes Controls Site-Specific DNA Amplifications.

Applications

Unspecified application

Species

Unspecified reactive species

Sweta Mishra,Capucine Van Rechem,Sangita Pal,Thomas L Clarke,Damayanti Chakraborty,Sarah D Mahan,Joshua C Black,Sedona E Murphy,Michael S Lawrence,Danette L Daniels,Johnathan R Whetstine

Molecular cell 70:707-721.e7 PubMed29754823

2018

Polε Instability Drives Replication Stress, Abnormal Development, and Tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Roberto Bellelli,Valerie Borel,Clare Logan,Jennifer Svendsen,Danielle E Cox,Emma Nye,Kay Metcalfe,Susan M O'Connell,Gordon Stamp,Helen R Flynn,Ambrosius P Snijders,François Lassailly,Andrew Jackson,Simon J Boulton

American journal of cancer research 8:39-55 PubMed29416919

2018

Mechanism of action of the atypical retinoid ST1926 in colorectal cancer: DNA damage and DNA polymerase α.

Applications

Unspecified application

Species

Unspecified reactive species

Rana Abdel-Samad,Patrick Aouad,Hala Gali-Muhtasib,Zeinab Sweidan,Raed Hmadi,Humam Kadara,Egildo Luca D'Andrea,Alessandra Fucci,Claudio Pisano,Nadine Darwiche

Nature communications 8:2130 PubMed29242643

2017

RNAs coordinate nuclear envelope assembly and DNA replication through ELYS recruitment to chromatin.

Applications

Unspecified application

Species

Unspecified reactive species

Antoine Aze,Michalis Fragkos,Stéphane Bocquet,Julien Cau,Marcel Méchali

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-DNA polymerase alpha/POLA antibody

Western blot - Anti-DNA polymerase alpha/POLA antibody (AB31777)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (14)

Product promise